Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion
The selection of a highly-viable single embryo in assisted reproductive technology requires an acceptable predictive method in order to reduce the multiple pregnancy rate and increase the success rate. In this study, the metabolomic profiling of growing and impaired embryos was assessed on the fifth...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/12/4094 |
| _version_ | 1797544233306947584 |
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| author | Marjan Motiei Katerina Vaculikova Andrea Cela Katerina Tvrdonova Reza Khalili David Rumpik Tatana Rumpikova Zdenek Glatz Tomas Saha |
| author_facet | Marjan Motiei Katerina Vaculikova Andrea Cela Katerina Tvrdonova Reza Khalili David Rumpik Tatana Rumpikova Zdenek Glatz Tomas Saha |
| author_sort | Marjan Motiei |
| collection | DOAJ |
| description | The selection of a highly-viable single embryo in assisted reproductive technology requires an acceptable predictive method in order to reduce the multiple pregnancy rate and increase the success rate. In this study, the metabolomic profiling of growing and impaired embryos was assessed on the fifth day of fertilization using capillary electrophoresis in order to find a relationship between the profiling and embryo development, and then to provide a mechanistic insight into the appearance/depletion of the metabolites. This unique qualitative technique exhibited the appearance of most non-essential amino acids and lactate, and depleting the serine, alanyl-glutamine and pyruvate in such a manner that the embryos impaired in their development secreted a considerably higher level of lactate and consumed a significantly higher amount of alanyl-glutamine. The different significant ratios of metabolomic depletion/appearance between the embryos confirm their potential for the improvement of the prospective selection of the developed single embryos, and also suggest the fact that pyruvate and alanyl-glutamine are the most critical ATP suppliers on the fifth day of blastocyst development. |
| first_indexed | 2024-03-10T13:57:30Z |
| format | Article |
| id | doaj.art-d24beab7e6a24eecafd4097554bf7a18 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T13:57:30Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-d24beab7e6a24eecafd4097554bf7a182023-11-21T01:32:19ZengMDPI AGJournal of Clinical Medicine2077-03832020-12-01912409410.3390/jcm9124094Non-Invasive Human Embryo Metabolic Assessment as a Developmental CriterionMarjan Motiei0Katerina Vaculikova1Andrea Cela2Katerina Tvrdonova3Reza Khalili4David Rumpik5Tatana Rumpikova6Zdenek Glatz7Tomas Saha8Centre of Polymer Systems, Tomas Bata University in Zlin, Třída Tomáše Bati 5678, 76001 Zlin, Czech RepublicFootwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou 3685, 76001 Zlin, Czech RepublicDepartment of Biochemistry, Faculty of Science, Masaryk University, 62500 Brno, Czech RepublicDepartment of Biology, Faculty of Sciences, University of Hradec Kralove, 50003 Hradec Kralove, Czech RepublicDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 2, 12808 Prague, Czech RepublicClinic of Reproductive Medicine and Gynaecology, 76001 Zlin, Czech RepublicClinic of Reproductive Medicine and Gynaecology, 76001 Zlin, Czech RepublicDepartment of Biochemistry, Faculty of Science, Masaryk University, 62500 Brno, Czech RepublicFootwear Research Centre, University Institute, Tomas Bata University in Zlin, Nad Ovcirnou 3685, 76001 Zlin, Czech RepublicThe selection of a highly-viable single embryo in assisted reproductive technology requires an acceptable predictive method in order to reduce the multiple pregnancy rate and increase the success rate. In this study, the metabolomic profiling of growing and impaired embryos was assessed on the fifth day of fertilization using capillary electrophoresis in order to find a relationship between the profiling and embryo development, and then to provide a mechanistic insight into the appearance/depletion of the metabolites. This unique qualitative technique exhibited the appearance of most non-essential amino acids and lactate, and depleting the serine, alanyl-glutamine and pyruvate in such a manner that the embryos impaired in their development secreted a considerably higher level of lactate and consumed a significantly higher amount of alanyl-glutamine. The different significant ratios of metabolomic depletion/appearance between the embryos confirm their potential for the improvement of the prospective selection of the developed single embryos, and also suggest the fact that pyruvate and alanyl-glutamine are the most critical ATP suppliers on the fifth day of blastocyst development.https://www.mdpi.com/2077-0383/9/12/4094metabolomicscapillary electrophoresisembryo developmentculture media |
| spellingShingle | Marjan Motiei Katerina Vaculikova Andrea Cela Katerina Tvrdonova Reza Khalili David Rumpik Tatana Rumpikova Zdenek Glatz Tomas Saha Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion Journal of Clinical Medicine metabolomics capillary electrophoresis embryo development culture media |
| title | Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion |
| title_full | Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion |
| title_fullStr | Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion |
| title_full_unstemmed | Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion |
| title_short | Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion |
| title_sort | non invasive human embryo metabolic assessment as a developmental criterion |
| topic | metabolomics capillary electrophoresis embryo development culture media |
| url | https://www.mdpi.com/2077-0383/9/12/4094 |
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