Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach.
The relationship between xylophagous termites and the protists resident in their hindguts is a textbook example of symbiosis. The essential steps of lignocellulose degradation handled by these protists allow the host termites to thrive on a wood diet. There has never been a comprehensive analysis of...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2797642?pdf=render |
| _version_ | 1819240007334887424 |
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| author | Nemuri Todaka Tetsushi Inoue Kanako Saita Moriya Ohkuma Christine A Nalepa Michael Lenz Toshiaki Kudo Shigeharu Moriya |
| author_facet | Nemuri Todaka Tetsushi Inoue Kanako Saita Moriya Ohkuma Christine A Nalepa Michael Lenz Toshiaki Kudo Shigeharu Moriya |
| author_sort | Nemuri Todaka |
| collection | DOAJ |
| description | The relationship between xylophagous termites and the protists resident in their hindguts is a textbook example of symbiosis. The essential steps of lignocellulose degradation handled by these protists allow the host termites to thrive on a wood diet. There has never been a comprehensive analysis of lignocellulose degradation by protists, however, as it has proven difficult to establish these symbionts in pure culture. The trends in lignocellulose degradation during the evolution of the host lineage are also largely unknown. To clarify these points without any cultivation technique, we performed meta-expressed sequence tag (EST) analysis of cDNA libraries originating from symbiotic protistan communities in four termite species and a wood-feeding cockroach. Our results reveal the establishment of a degradation system with multiple enzymes at the ancestral stage of termite-protistan symbiosis, especially GHF5 and 7. According to our phylogenetic analyses, the enzymes comprising the protistan lignocellulose degradation system are coded not only by genes innate to the protists, but also genes acquired by the protists via lateral transfer from bacteria. This gives us a fresh perspective from which to understand the evolutionary dynamics of symbiosis. |
| first_indexed | 2024-12-23T14:01:10Z |
| format | Article |
| id | doaj.art-d24cfeff10074a86b4b3650e29766153 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:01:10Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d24cfeff10074a86b4b3650e297661532022-12-21T17:44:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0151e863610.1371/journal.pone.0008636Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach.Nemuri TodakaTetsushi InoueKanako SaitaMoriya OhkumaChristine A NalepaMichael LenzToshiaki KudoShigeharu MoriyaThe relationship between xylophagous termites and the protists resident in their hindguts is a textbook example of symbiosis. The essential steps of lignocellulose degradation handled by these protists allow the host termites to thrive on a wood diet. There has never been a comprehensive analysis of lignocellulose degradation by protists, however, as it has proven difficult to establish these symbionts in pure culture. The trends in lignocellulose degradation during the evolution of the host lineage are also largely unknown. To clarify these points without any cultivation technique, we performed meta-expressed sequence tag (EST) analysis of cDNA libraries originating from symbiotic protistan communities in four termite species and a wood-feeding cockroach. Our results reveal the establishment of a degradation system with multiple enzymes at the ancestral stage of termite-protistan symbiosis, especially GHF5 and 7. According to our phylogenetic analyses, the enzymes comprising the protistan lignocellulose degradation system are coded not only by genes innate to the protists, but also genes acquired by the protists via lateral transfer from bacteria. This gives us a fresh perspective from which to understand the evolutionary dynamics of symbiosis.http://europepmc.org/articles/PMC2797642?pdf=render |
| spellingShingle | Nemuri Todaka Tetsushi Inoue Kanako Saita Moriya Ohkuma Christine A Nalepa Michael Lenz Toshiaki Kudo Shigeharu Moriya Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. PLoS ONE |
| title | Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. |
| title_full | Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. |
| title_fullStr | Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. |
| title_full_unstemmed | Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. |
| title_short | Phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach. |
| title_sort | phylogenetic analysis of cellulolytic enzyme genes from representative lineages of termites and a related cockroach |
| url | http://europepmc.org/articles/PMC2797642?pdf=render |
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