Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the phar...
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Language: | English |
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Elsevier
2022-03-01
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Series: | Ecotoxicology and Environmental Safety |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322001798 |
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author | Xiao Wang Wei Liu Guozhong Jin Zhanjun Wu Di Zhang Yongzhan Bao Wanyu Shi |
author_facet | Xiao Wang Wei Liu Guozhong Jin Zhanjun Wu Di Zhang Yongzhan Bao Wanyu Shi |
author_sort | Xiao Wang |
collection | DOAJ |
description | Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the pharmacological effects of regulating immunity and protecting the liver of animals, and its alleviative effect on renal injury is unclear. In order to investigate the alleviating effect of SMPs on drug nephrotoxicity and determine its potential molecular mechanism, we took chicks as the research object, FFC as the induced drug, and established the model by adding SMPs in drinking water. The chicks were randomly divided into control group, FFC model group (0.15 g/L FFC), FFC + low, medium and high dose of SMPs groups (0.15 g/L FFC + 1.25, 2.5, 5 g/L SMPs) and SMPs group (5 g/L SMPs). The results showed that, SMPs increased the average weight gain and renal index of chicks, alleviated the pathological changes of renal structure induced by FFC, decreased the contents of uric acid, blood urea nitrogen and creatinine in serum and malondialdehyde in renal tissue, increased the levels of glutathione, superoxide dismutase and catalase in renal tissue, up-regulated the relative expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1) mRNA and protein, and down-regulated the relative expression levels of p53, Caspase-3 and Caspase-6 mRNA and protein and the apoptosis rate of renal histiocytes. It is concluded that SMPs could significantly alleviate the renal injury induced by FFC, and its mechanism may be related to improving renal antioxidant capacity and inhibiting abnormal apoptosis of renal histiocytes. |
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issn | 0147-6513 |
language | English |
last_indexed | 2024-12-22T06:37:56Z |
publishDate | 2022-03-01 |
publisher | Elsevier |
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series | Ecotoxicology and Environmental Safety |
spelling | doaj.art-d24e22700ca14d25a3de53656ff31fea2022-12-21T18:35:32ZengElsevierEcotoxicology and Environmental Safety0147-65132022-03-01233113339Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosisXiao Wang0Wei Liu1Guozhong Jin2Zhanjun Wu3Di Zhang4Yongzhan Bao5Wanyu Shi6College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China; Hebei Provincial Veterinary Biotechnology Innovation Center, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China; Hebei Provincial Veterinary Biotechnology Innovation Center, Baoding 071001, China; Correspondence to: Hebei Agricultural University, 2596# Le Kai South Street, Baoding 071001, Hebei, China.Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the pharmacological effects of regulating immunity and protecting the liver of animals, and its alleviative effect on renal injury is unclear. In order to investigate the alleviating effect of SMPs on drug nephrotoxicity and determine its potential molecular mechanism, we took chicks as the research object, FFC as the induced drug, and established the model by adding SMPs in drinking water. The chicks were randomly divided into control group, FFC model group (0.15 g/L FFC), FFC + low, medium and high dose of SMPs groups (0.15 g/L FFC + 1.25, 2.5, 5 g/L SMPs) and SMPs group (5 g/L SMPs). The results showed that, SMPs increased the average weight gain and renal index of chicks, alleviated the pathological changes of renal structure induced by FFC, decreased the contents of uric acid, blood urea nitrogen and creatinine in serum and malondialdehyde in renal tissue, increased the levels of glutathione, superoxide dismutase and catalase in renal tissue, up-regulated the relative expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1) mRNA and protein, and down-regulated the relative expression levels of p53, Caspase-3 and Caspase-6 mRNA and protein and the apoptosis rate of renal histiocytes. It is concluded that SMPs could significantly alleviate the renal injury induced by FFC, and its mechanism may be related to improving renal antioxidant capacity and inhibiting abnormal apoptosis of renal histiocytes.http://www.sciencedirect.com/science/article/pii/S0147651322001798Salvia miltiorrhiza polysaccharidesFlorfenicolChickNephrotoxicityOxidative stressApoptosis |
spellingShingle | Xiao Wang Wei Liu Guozhong Jin Zhanjun Wu Di Zhang Yongzhan Bao Wanyu Shi Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis Ecotoxicology and Environmental Safety Salvia miltiorrhiza polysaccharides Florfenicol Chick Nephrotoxicity Oxidative stress Apoptosis |
title | Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
title_full | Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
title_fullStr | Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
title_full_unstemmed | Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
title_short | Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
title_sort | salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis |
topic | Salvia miltiorrhiza polysaccharides Florfenicol Chick Nephrotoxicity Oxidative stress Apoptosis |
url | http://www.sciencedirect.com/science/article/pii/S0147651322001798 |
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