Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis

Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the phar...

Full description

Bibliographic Details
Main Authors: Xiao Wang, Wei Liu, Guozhong Jin, Zhanjun Wu, Di Zhang, Yongzhan Bao, Wanyu Shi
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:Ecotoxicology and Environmental Safety
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0147651322001798
_version_ 1819121524802584576
author Xiao Wang
Wei Liu
Guozhong Jin
Zhanjun Wu
Di Zhang
Yongzhan Bao
Wanyu Shi
author_facet Xiao Wang
Wei Liu
Guozhong Jin
Zhanjun Wu
Di Zhang
Yongzhan Bao
Wanyu Shi
author_sort Xiao Wang
collection DOAJ
description Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the pharmacological effects of regulating immunity and protecting the liver of animals, and its alleviative effect on renal injury is unclear. In order to investigate the alleviating effect of SMPs on drug nephrotoxicity and determine its potential molecular mechanism, we took chicks as the research object, FFC as the induced drug, and established the model by adding SMPs in drinking water. The chicks were randomly divided into control group, FFC model group (0.15 g/L FFC), FFC + low, medium and high dose of SMPs groups (0.15 g/L FFC + 1.25, 2.5, 5 g/L SMPs) and SMPs group (5 g/L SMPs). The results showed that, SMPs increased the average weight gain and renal index of chicks, alleviated the pathological changes of renal structure induced by FFC, decreased the contents of uric acid, blood urea nitrogen and creatinine in serum and malondialdehyde in renal tissue, increased the levels of glutathione, superoxide dismutase and catalase in renal tissue, up-regulated the relative expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1) mRNA and protein, and down-regulated the relative expression levels of p53, Caspase-3 and Caspase-6 mRNA and protein and the apoptosis rate of renal histiocytes. It is concluded that SMPs could significantly alleviate the renal injury induced by FFC, and its mechanism may be related to improving renal antioxidant capacity and inhibiting abnormal apoptosis of renal histiocytes.
first_indexed 2024-12-22T06:37:56Z
format Article
id doaj.art-d24e22700ca14d25a3de53656ff31fea
institution Directory Open Access Journal
issn 0147-6513
language English
last_indexed 2024-12-22T06:37:56Z
publishDate 2022-03-01
publisher Elsevier
record_format Article
series Ecotoxicology and Environmental Safety
spelling doaj.art-d24e22700ca14d25a3de53656ff31fea2022-12-21T18:35:32ZengElsevierEcotoxicology and Environmental Safety0147-65132022-03-01233113339Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosisXiao Wang0Wei Liu1Guozhong Jin2Zhanjun Wu3Di Zhang4Yongzhan Bao5Wanyu Shi6College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China; Hebei Provincial Veterinary Biotechnology Innovation Center, Baoding 071001, ChinaCollege of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China; Hebei Provincial Veterinary Biotechnology Innovation Center, Baoding 071001, China; Correspondence to: Hebei Agricultural University, 2596# Le Kai South Street, Baoding 071001, Hebei, China.Florfenicol (FFC) is a commonly used antibiotic in animal husbandry, which is easy to cause organs damage in a variety of animals. It has been proved to have nephrotoxicity and affect the yield and quality of meat products. Salvia miltiorrhiza polysaccharides (SMPs) have been proved to have the pharmacological effects of regulating immunity and protecting the liver of animals, and its alleviative effect on renal injury is unclear. In order to investigate the alleviating effect of SMPs on drug nephrotoxicity and determine its potential molecular mechanism, we took chicks as the research object, FFC as the induced drug, and established the model by adding SMPs in drinking water. The chicks were randomly divided into control group, FFC model group (0.15 g/L FFC), FFC + low, medium and high dose of SMPs groups (0.15 g/L FFC + 1.25, 2.5, 5 g/L SMPs) and SMPs group (5 g/L SMPs). The results showed that, SMPs increased the average weight gain and renal index of chicks, alleviated the pathological changes of renal structure induced by FFC, decreased the contents of uric acid, blood urea nitrogen and creatinine in serum and malondialdehyde in renal tissue, increased the levels of glutathione, superoxide dismutase and catalase in renal tissue, up-regulated the relative expression levels of nuclear factor erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1) and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1) mRNA and protein, and down-regulated the relative expression levels of p53, Caspase-3 and Caspase-6 mRNA and protein and the apoptosis rate of renal histiocytes. It is concluded that SMPs could significantly alleviate the renal injury induced by FFC, and its mechanism may be related to improving renal antioxidant capacity and inhibiting abnormal apoptosis of renal histiocytes.http://www.sciencedirect.com/science/article/pii/S0147651322001798Salvia miltiorrhiza polysaccharidesFlorfenicolChickNephrotoxicityOxidative stressApoptosis
spellingShingle Xiao Wang
Wei Liu
Guozhong Jin
Zhanjun Wu
Di Zhang
Yongzhan Bao
Wanyu Shi
Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
Ecotoxicology and Environmental Safety
Salvia miltiorrhiza polysaccharides
Florfenicol
Chick
Nephrotoxicity
Oxidative stress
Apoptosis
title Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
title_full Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
title_fullStr Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
title_full_unstemmed Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
title_short Salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
title_sort salvia miltiorrhiza polysaccharides alleviates florfenicol induced kidney injury in chicks via inhibiting oxidative stress and apoptosis
topic Salvia miltiorrhiza polysaccharides
Florfenicol
Chick
Nephrotoxicity
Oxidative stress
Apoptosis
url http://www.sciencedirect.com/science/article/pii/S0147651322001798
work_keys_str_mv AT xiaowang salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT weiliu salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT guozhongjin salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT zhanjunwu salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT dizhang salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT yongzhanbao salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis
AT wanyushi salviamiltiorrhizapolysaccharidesalleviatesflorfenicolinducedkidneyinjuryinchicksviainhibitingoxidativestressandapoptosis