Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser
Conjunctivochalasis is a degenerative condition of the conjunctiva that disrupts tear distribution and causes irritation. Thermoreduction of the redundant conjunctiva is required if symptoms are not relieved with medical therapy. Near-infrared laser treatment is a more controlled method to shrink th...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5740 |
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| author | Rodrigo Guimaraes de Souza David Huang Scott Prahl Lauren Nakhleh Stephen C. Pflugfelder |
| author_facet | Rodrigo Guimaraes de Souza David Huang Scott Prahl Lauren Nakhleh Stephen C. Pflugfelder |
| author_sort | Rodrigo Guimaraes de Souza |
| collection | DOAJ |
| description | Conjunctivochalasis is a degenerative condition of the conjunctiva that disrupts tear distribution and causes irritation. Thermoreduction of the redundant conjunctiva is required if symptoms are not relieved with medical therapy. Near-infrared laser treatment is a more controlled method to shrink the conjunctiva than thermocautery. This study compared tissue shrinkage, histology, and postoperative inflammation in thermoconjunctivoplasty performed on the mouse conjunctiva using either thermocautery or pulsed 1460 nm near-infrared laser irradiation. Three sets of experiments were performed on female C57BL/6J mice (<i>n</i> = 72, 26 per treatment group and 20 control) to assess conjunctival shrinkage, wound histology, and inflammation 3 and 10 days after treatment. Both treatments effectively shrunk the conjunctiva, but thermocautery caused greater epithelial damage. Thermocautery caused greater infiltration of neutrophils on day 3 and neutrophils and CD11b<sup>+</sup> myeloid cells on day 10. The thermocautery group had significantly higher conjunctival expression of <i>IL-1β</i> on day 3. Expression of chemokine CCL2 was higher in the conjunctiva on day 3 and tear concentrations were higher on day 7 in the laser group. These results suggest that pulsed laser treatment causes less tissue damage and postoperative inflammation than thermocautery while effectively addressing conjunctivochalasis. |
| first_indexed | 2024-03-11T06:25:27Z |
| format | Article |
| id | doaj.art-d251bd2dbbc4496183d3542b882860d9 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:25:27Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d251bd2dbbc4496183d3542b882860d92023-11-17T11:38:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246574010.3390/ijms24065740Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm LaserRodrigo Guimaraes de Souza0David Huang1Scott Prahl2Lauren Nakhleh3Stephen C. Pflugfelder4Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USADepartment of Ophthalmology, Oregon Health Sciences Center, Portland, OR 97239, USADepartment of Electrical Engineering and Renewable Energy, Oregon Institute of Technology, Wilsonville, OR 97070, USADepartment of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USADepartment of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USAConjunctivochalasis is a degenerative condition of the conjunctiva that disrupts tear distribution and causes irritation. Thermoreduction of the redundant conjunctiva is required if symptoms are not relieved with medical therapy. Near-infrared laser treatment is a more controlled method to shrink the conjunctiva than thermocautery. This study compared tissue shrinkage, histology, and postoperative inflammation in thermoconjunctivoplasty performed on the mouse conjunctiva using either thermocautery or pulsed 1460 nm near-infrared laser irradiation. Three sets of experiments were performed on female C57BL/6J mice (<i>n</i> = 72, 26 per treatment group and 20 control) to assess conjunctival shrinkage, wound histology, and inflammation 3 and 10 days after treatment. Both treatments effectively shrunk the conjunctiva, but thermocautery caused greater epithelial damage. Thermocautery caused greater infiltration of neutrophils on day 3 and neutrophils and CD11b<sup>+</sup> myeloid cells on day 10. The thermocautery group had significantly higher conjunctival expression of <i>IL-1β</i> on day 3. Expression of chemokine CCL2 was higher in the conjunctiva on day 3 and tear concentrations were higher on day 7 in the laser group. These results suggest that pulsed laser treatment causes less tissue damage and postoperative inflammation than thermocautery while effectively addressing conjunctivochalasis.https://www.mdpi.com/1422-0067/24/6/5740conjunctivochalasislasernear-infraredthermocauterythermoconjunctivoplasty |
| spellingShingle | Rodrigo Guimaraes de Souza David Huang Scott Prahl Lauren Nakhleh Stephen C. Pflugfelder Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser International Journal of Molecular Sciences conjunctivochalasis laser near-infrared thermocautery thermoconjunctivoplasty |
| title | Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser |
| title_full | Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser |
| title_fullStr | Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser |
| title_full_unstemmed | Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser |
| title_short | Comparison of Efficacy and Inflammatory Response to Thermoconjunctivoplasty Performed with Cautery or Pulsed 1460 nm Laser |
| title_sort | comparison of efficacy and inflammatory response to thermoconjunctivoplasty performed with cautery or pulsed 1460 nm laser |
| topic | conjunctivochalasis laser near-infrared thermocautery thermoconjunctivoplasty |
| url | https://www.mdpi.com/1422-0067/24/6/5740 |
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