High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight
Northern corn leaf blight (NCLB), caused by the fungal pathogen Exserohilum turcicum, poses a grave threat to maize production worldwide. The resistance gene in A619Ht3, discovered decades ago, is an important genetic resource for NCLB control. By using a pair of near-isogenic lines (NILs) A619Ht3 a...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.968924/full |
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| author | Mang Zhu Jun Ma Xinfang Liu Yanling Guo Xin Qi Xue Gong Yanbin Zhu Yanbin Zhu Yanbo Wang Min Jiang |
| author_facet | Mang Zhu Jun Ma Xinfang Liu Yanling Guo Xin Qi Xue Gong Yanbin Zhu Yanbin Zhu Yanbo Wang Min Jiang |
| author_sort | Mang Zhu |
| collection | DOAJ |
| description | Northern corn leaf blight (NCLB), caused by the fungal pathogen Exserohilum turcicum, poses a grave threat to maize production worldwide. The resistance gene in A619Ht3, discovered decades ago, is an important genetic resource for NCLB control. By using a pair of near-isogenic lines (NILs) A619Ht3 and A619, together with the resistant and susceptible bulks derived from the cross of A619Ht3 and L3162 lines, we initially detected a Ht3-like (Ht3L) locus in bin 8.06 that was closely associated with NCLB resistance. We then performed five rounds of fine-mapping, which ultimately delimited the Ht3L locus to a 577-kb interval flanked by SNP markers KA002081 and KA002084. Plants homozygous for the Ht3L/Ht3L genotype exhibited an average reduction in diseased leaf area (DLA) by 16.5% compared to plants lacking Ht3L locus. The Ht3L locus showed extensive variation in genomic architecture among different maize lines and did not appear to contain any genes encoding canonical cell wall-associated kinases against NCLB. Moreover, the Ht3L locus was located ∼2.7 Mb away from the known Htn1 locus. We speculate that the Ht3L locus may contain a bona fide Ht3 gene or a novel NCLB resistance gene closely linked to Ht3. In practice, the Ht3L locus is a valuable resource for improving maize resistance to NCLB. |
| first_indexed | 2024-04-12T05:00:39Z |
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| id | doaj.art-d2521725932f4bb28d709f10c85ac035 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T05:00:39Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-d2521725932f4bb28d709f10c85ac0352022-12-22T03:47:01ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.968924968924High-resolution mapping reveals a Ht3-like locus against northern corn leaf blightMang Zhu0Jun Ma1Xinfang Liu2Yanling Guo3Xin Qi4Xue Gong5Yanbin Zhu6Yanbin Zhu7Yanbo Wang8Min Jiang9State Key Laboratory of Plant Physiology and Biochemistry, College of Agronomy and Biotechnology, National Maize Improvement Center, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Dongya Agricultural Development Co., Ltd., Shenyang, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaState Key Laboratory of Plant Physiology and Biochemistry, College of Agronomy and Biotechnology, National Maize Improvement Center, Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing, ChinaLiaoning Dongya Agricultural Development Co., Ltd., Shenyang, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaLiaoning Academy of Agricultural Sciences, Shenyang, ChinaNorthern corn leaf blight (NCLB), caused by the fungal pathogen Exserohilum turcicum, poses a grave threat to maize production worldwide. The resistance gene in A619Ht3, discovered decades ago, is an important genetic resource for NCLB control. By using a pair of near-isogenic lines (NILs) A619Ht3 and A619, together with the resistant and susceptible bulks derived from the cross of A619Ht3 and L3162 lines, we initially detected a Ht3-like (Ht3L) locus in bin 8.06 that was closely associated with NCLB resistance. We then performed five rounds of fine-mapping, which ultimately delimited the Ht3L locus to a 577-kb interval flanked by SNP markers KA002081 and KA002084. Plants homozygous for the Ht3L/Ht3L genotype exhibited an average reduction in diseased leaf area (DLA) by 16.5% compared to plants lacking Ht3L locus. The Ht3L locus showed extensive variation in genomic architecture among different maize lines and did not appear to contain any genes encoding canonical cell wall-associated kinases against NCLB. Moreover, the Ht3L locus was located ∼2.7 Mb away from the known Htn1 locus. We speculate that the Ht3L locus may contain a bona fide Ht3 gene or a novel NCLB resistance gene closely linked to Ht3. In practice, the Ht3L locus is a valuable resource for improving maize resistance to NCLB.https://www.frontiersin.org/articles/10.3389/fpls.2022.968924/fullHt3northern corn leaf blight (NCLB)maize (Zea mays L.)maize diseasefine-mapping |
| spellingShingle | Mang Zhu Jun Ma Xinfang Liu Yanling Guo Xin Qi Xue Gong Yanbin Zhu Yanbin Zhu Yanbo Wang Min Jiang High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight Frontiers in Plant Science Ht3 northern corn leaf blight (NCLB) maize (Zea mays L.) maize disease fine-mapping |
| title | High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight |
| title_full | High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight |
| title_fullStr | High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight |
| title_full_unstemmed | High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight |
| title_short | High-resolution mapping reveals a Ht3-like locus against northern corn leaf blight |
| title_sort | high resolution mapping reveals a ht3 like locus against northern corn leaf blight |
| topic | Ht3 northern corn leaf blight (NCLB) maize (Zea mays L.) maize disease fine-mapping |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.968924/full |
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