Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system.
Spatial arrangement of chromosomes is responsible for gene expression in Plasmodium parasites. However, methods for rearranging chromosomes have not been established, which makes it difficult to investigate its role in detail. Here, we report a method for splitting chromosome in rodent malaria paras...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0260176 |
| _version_ | 1819120040102854656 |
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| author | Daniel Addo-Gyan Haruka Matsushita Enya Sora Tsubasa Nishi Masao Yuda Naoaki Shinzawa Shiroh Iwanaga |
| author_facet | Daniel Addo-Gyan Haruka Matsushita Enya Sora Tsubasa Nishi Masao Yuda Naoaki Shinzawa Shiroh Iwanaga |
| author_sort | Daniel Addo-Gyan |
| collection | DOAJ |
| description | Spatial arrangement of chromosomes is responsible for gene expression in Plasmodium parasites. However, methods for rearranging chromosomes have not been established, which makes it difficult to investigate its role in detail. Here, we report a method for splitting chromosome in rodent malaria parasite by CRISPR/Cas9 system using fragments in which a telomere and a centromere were incorporated. The resultant split chromosomes segregated accurately into daughter parasites by the centromere. In addition, elongation of de novo telomeres were observed, indicating its proper function. Furthermore, chromosome splitting had no effect on development of parasites. Splitting of the chromosome is expected to alter its spatial arrangement, and our method will thus be useful for investigating its biological role related with gene expression. |
| first_indexed | 2024-12-22T06:14:20Z |
| format | Article |
| id | doaj.art-d2526f5a4eca4916af4e3ff91c8d4ab2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T06:14:20Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d2526f5a4eca4916af4e3ff91c8d4ab22022-12-21T18:36:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01172e026017610.1371/journal.pone.0260176Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system.Daniel Addo-GyanHaruka MatsushitaEnya SoraTsubasa NishiMasao YudaNaoaki ShinzawaShiroh IwanagaSpatial arrangement of chromosomes is responsible for gene expression in Plasmodium parasites. However, methods for rearranging chromosomes have not been established, which makes it difficult to investigate its role in detail. Here, we report a method for splitting chromosome in rodent malaria parasite by CRISPR/Cas9 system using fragments in which a telomere and a centromere were incorporated. The resultant split chromosomes segregated accurately into daughter parasites by the centromere. In addition, elongation of de novo telomeres were observed, indicating its proper function. Furthermore, chromosome splitting had no effect on development of parasites. Splitting of the chromosome is expected to alter its spatial arrangement, and our method will thus be useful for investigating its biological role related with gene expression.https://doi.org/10.1371/journal.pone.0260176 |
| spellingShingle | Daniel Addo-Gyan Haruka Matsushita Enya Sora Tsubasa Nishi Masao Yuda Naoaki Shinzawa Shiroh Iwanaga Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. PLoS ONE |
| title | Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. |
| title_full | Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. |
| title_fullStr | Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. |
| title_full_unstemmed | Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. |
| title_short | Chromosome splitting of Plasmodium berghei using the CRISPR/Cas9 system. |
| title_sort | chromosome splitting of plasmodium berghei using the crispr cas9 system |
| url | https://doi.org/10.1371/journal.pone.0260176 |
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