A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1
The plasma membrane (PM) is at the interface of plant–pathogen interactions and, thus, many bacterial type-III effector (T3E) proteins target membrane-associated processes to interfere with immunity. The Pseudomonas syringae T3E HopZ1a is a host cell PM-localized effector protein that has several im...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2019-09-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-19-0105-R |
| _version_ | 1811264539951366144 |
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| author | Philip Albers Suayib Üstün Katja Witzel Max Kraner Frederik Börnke |
| author_facet | Philip Albers Suayib Üstün Katja Witzel Max Kraner Frederik Börnke |
| author_sort | Philip Albers |
| collection | DOAJ |
| description | The plasma membrane (PM) is at the interface of plant–pathogen interactions and, thus, many bacterial type-III effector (T3E) proteins target membrane-associated processes to interfere with immunity. The Pseudomonas syringae T3E HopZ1a is a host cell PM-localized effector protein that has several immunity-associated host targets but also activates effector-triggered immunity in resistant backgrounds. Although HopZ1a has been shown to interfere with early defense signaling at the PM, no dedicated PM-associated HopZ1a target protein has been identified until now. Here, we show that HopZ1a interacts with the PM-associated remorin protein NbREM4 from Nicotiana benthamiana in several independent assays. NbREM4 relocalizes to membrane nanodomains after treatment with the bacterial elicitor flg22 and transient overexpression of NbREM4 in N. benthamiana induces the expression of a subset of defense-related genes. We can further show that NbREM4 interacts with the immune-related receptor-like cytoplasmic kinase avrPphB-susceptible 1 (PBS1) and is phosphorylated by PBS1 on several residues in vitro. Thus, we conclude that NbREM4 is associated with early defense signaling at the PM. The possible relevance of the HopZ1a–NbREM4 interaction for HopZ1a virulence and avirulence functions is discussed.[Graphic: see text]Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-04-12T20:04:59Z |
| format | Article |
| id | doaj.art-d256e95e6227478d98f409ddd52e617d |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-12T20:04:59Z |
| publishDate | 2019-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d256e95e6227478d98f409ddd52e617d2022-12-22T03:18:25ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062019-09-013291229124210.1094/MPMI-04-19-0105-RA Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1Philip Albers0Suayib Üstün1Katja Witzel2Max Kraner3Frederik Börnke4Plant Metabolism, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, GermanyPlant Metabolism, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, GermanyPrinciples of Integrated Pest Management, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, GermanyFriedrich-Alexander-Universität, Department of Biology, Division of Biochemistry, 91058 Erlangen, GermanyPlant Metabolism, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), 14979 Großbeeren, GermanyThe plasma membrane (PM) is at the interface of plant–pathogen interactions and, thus, many bacterial type-III effector (T3E) proteins target membrane-associated processes to interfere with immunity. The Pseudomonas syringae T3E HopZ1a is a host cell PM-localized effector protein that has several immunity-associated host targets but also activates effector-triggered immunity in resistant backgrounds. Although HopZ1a has been shown to interfere with early defense signaling at the PM, no dedicated PM-associated HopZ1a target protein has been identified until now. Here, we show that HopZ1a interacts with the PM-associated remorin protein NbREM4 from Nicotiana benthamiana in several independent assays. NbREM4 relocalizes to membrane nanodomains after treatment with the bacterial elicitor flg22 and transient overexpression of NbREM4 in N. benthamiana induces the expression of a subset of defense-related genes. We can further show that NbREM4 interacts with the immune-related receptor-like cytoplasmic kinase avrPphB-susceptible 1 (PBS1) and is phosphorylated by PBS1 on several residues in vitro. Thus, we conclude that NbREM4 is associated with early defense signaling at the PM. The possible relevance of the HopZ1a–NbREM4 interaction for HopZ1a virulence and avirulence functions is discussed.[Graphic: see text]Copyright © 2019 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-19-0105-Rbacterial pathogenesisdefense signaling pathwayseffectorselicitorsHopZ1aMAMPs |
| spellingShingle | Philip Albers Suayib Üstün Katja Witzel Max Kraner Frederik Börnke A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 Molecular Plant-Microbe Interactions bacterial pathogenesis defense signaling pathways effectors elicitors HopZ1a MAMPs |
| title | A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 |
| title_full | A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 |
| title_fullStr | A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 |
| title_full_unstemmed | A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 |
| title_short | A Remorin from Nicotiana benthamiana Interacts with the Pseudomonas Type-III Effector Protein HopZ1a and is Phosphorylated by the Immune-Related Kinase PBS1 |
| title_sort | remorin from nicotiana benthamiana interacts with the pseudomonas type iii effector protein hopz1a and is phosphorylated by the immune related kinase pbs1 |
| topic | bacterial pathogenesis defense signaling pathways effectors elicitors HopZ1a MAMPs |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-04-19-0105-R |
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