Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing
Problems that often arise in the process of freezing semen is destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that reduce damage due to peroxidation. The purpose of the stud...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2018-06-01
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| Series: | Journal of Innovation and Applied Technology |
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| Online Access: | https://jiat.ub.ac.id/index.php/jiat/article/view/140 |
| _version_ | 1818522256897212416 |
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| author | Sri Wahjuningsih |
| author_facet | Sri Wahjuningsih |
| author_sort | Sri Wahjuningsih |
| collection | DOAJ |
| description | Problems that often arise in the process of freezing semen is destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on plasma membrane integrity after freezing process. Semen was collected using artificial vagina from goat aged 2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage to plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%). Plasma membrane integrity examination results at post thawing at dose of 0.4 g (73.16%)was higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain membrane integrity of semen frozen of goat. |
| first_indexed | 2024-12-11T05:30:52Z |
| format | Article |
| id | doaj.art-d258727548d04193861d88fd09442d65 |
| institution | Directory Open Access Journal |
| issn | 2502-4973 2477-7951 |
| language | English |
| last_indexed | 2024-12-11T05:30:52Z |
| publishDate | 2018-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Innovation and Applied Technology |
| spelling | doaj.art-d258727548d04193861d88fd09442d652022-12-22T01:19:26ZengUniversity of BrawijayaJournal of Innovation and Applied Technology2502-49732477-79512018-06-014158158510.21776/ub.jiat.004.01.797Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After FreezingSri Wahjuningsih0University of BrawijayaProblems that often arise in the process of freezing semen is destruction of the plasma membrane because the sperm membrane contains unsaturated fatty acids are highly susceptible to peroxidation damage. α-tocopherol is an antioxidant that reduce damage due to peroxidation. The purpose of the study was to determine α-tocopherol antioxidant supplementation in diluter on plasma membrane integrity after freezing process. Semen was collected using artificial vagina from goat aged 2 to 2.5 years in normal reproduction. The design used was randomized block design with treatment dose of a different α-tocopherol (0 grams, 0.2 grams, 0.4 grams, 0.6 grams) in 100 ml of extender. The results showed that the percentage to plasma membrane integrity equilibrated at a dose of 0.4 g (75.46%) was higher (P <0.05) compared with a dose of 0.0 g (73.78%), and the dose of 0.6 g (72.93%), but did not differ (P> 0.05 ) with a dose of 0.2 g (75.46%). Plasma membrane integrity examination results at post thawing at dose of 0.4 g (73.16%)was higher (P <0.05) than a dose of 0.0 g (69.8%), 0.2 g (71.53%), 0.6 g (70.7 %) respectively. It was concluded that supplementation of antioxidant α-tocopherol 0.4 ml is the best dose to maintain membrane integrity of semen frozen of goat.https://jiat.ub.ac.id/index.php/jiat/article/view/140antioxidant, plasma membrane integrity, goat semen |
| spellingShingle | Sri Wahjuningsih Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing Journal of Innovation and Applied Technology antioxidant, plasma membrane integrity, goat semen |
| title | Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing |
| title_full | Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing |
| title_fullStr | Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing |
| title_full_unstemmed | Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing |
| title_short | Supplementation Of α Tocopherol On Plasma Membrane Integrity Of Goat Sperm After Freezing |
| title_sort | supplementation of α tocopherol on plasma membrane integrity of goat sperm after freezing |
| topic | antioxidant, plasma membrane integrity, goat semen |
| url | https://jiat.ub.ac.id/index.php/jiat/article/view/140 |
| work_keys_str_mv | AT sriwahjuningsih supplementationofatocopherolonplasmamembraneintegrityofgoatspermafterfreezing |