Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins
The effects of organic modifiers—alcohols (methanol, isopropanol), acetonitrile, and tetrahydrofuran—and β-cyclodextrins in capillary zone electrophoresis were investigated using a test mixture containing five biogenic amines important from the human health point of view—serotonin, dopamine, adrenal...
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| Format: | Article |
| Language: | English |
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Sciendo
2022-08-01
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| Series: | European Pharmaceutical Journal |
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| Online Access: | https://doi.org/10.2478/afpuc-2022-0012 |
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| author | Matuskova M. Cizmarova I. Chalova P. Stefanik O. Horniakova A. Mikus P. Piestansky J. |
| author_facet | Matuskova M. Cizmarova I. Chalova P. Stefanik O. Horniakova A. Mikus P. Piestansky J. |
| author_sort | Matuskova M. |
| collection | DOAJ |
| description | The effects of organic modifiers—alcohols (methanol, isopropanol), acetonitrile, and tetrahydrofuran—and β-cyclodextrins in capillary zone electrophoresis were investigated using a test mixture containing five biogenic amines important from the human health point of view—serotonin, dopamine, adrenaline, noradrenaline, and tyramine—and two B vitamins—thiamine and pyridoxine. The simultaneous addition of tetrahydrofuran and isopropanol was found to improve the resolution of determined analytes and enable effective separation of analytes with very similar electromigration characteristics migrating as one peak (dopamine and serotonin). The developed and optimised separation method based on capillary zone electrophoresis and ultraviolet detection was capable to achieve detection limits at the concentration level in the range of 0.15 to 1.25 μg/mL−1. The developed method was also characterised by other favourable validation parameters, such as linearity (r2 > 0.99), accuracy (82.9–117.8% for the intraday measurements, and 87.6–119.2% for the interday measurements), and precision (intraday relative standard deviation in the range of 0.4–15.5%, interday relative standard deviation in the range of 0.9–18.3%). The method was finally applied to investigate the stability of the analytes in model water matrix samples under various storage conditions. |
| first_indexed | 2024-04-11T22:40:04Z |
| format | Article |
| id | doaj.art-d2599c78d77e45d8af70e575d2fb6f31 |
| institution | Directory Open Access Journal |
| issn | 2453-6725 |
| language | English |
| last_indexed | 2024-04-11T22:40:04Z |
| publishDate | 2022-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | European Pharmaceutical Journal |
| spelling | doaj.art-d2599c78d77e45d8af70e575d2fb6f312022-12-22T03:59:02ZengSciendoEuropean Pharmaceutical Journal2453-67252022-08-01691435310.2478/afpuc-2022-0012Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B VitaminsMatuskova M.0Cizmarova I.1Chalova P.2Stefanik O.3Horniakova A.4Mikus P.5Piestansky J.6Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaDepartment of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, SlovakiaThe effects of organic modifiers—alcohols (methanol, isopropanol), acetonitrile, and tetrahydrofuran—and β-cyclodextrins in capillary zone electrophoresis were investigated using a test mixture containing five biogenic amines important from the human health point of view—serotonin, dopamine, adrenaline, noradrenaline, and tyramine—and two B vitamins—thiamine and pyridoxine. The simultaneous addition of tetrahydrofuran and isopropanol was found to improve the resolution of determined analytes and enable effective separation of analytes with very similar electromigration characteristics migrating as one peak (dopamine and serotonin). The developed and optimised separation method based on capillary zone electrophoresis and ultraviolet detection was capable to achieve detection limits at the concentration level in the range of 0.15 to 1.25 μg/mL−1. The developed method was also characterised by other favourable validation parameters, such as linearity (r2 > 0.99), accuracy (82.9–117.8% for the intraday measurements, and 87.6–119.2% for the interday measurements), and precision (intraday relative standard deviation in the range of 0.4–15.5%, interday relative standard deviation in the range of 0.9–18.3%). The method was finally applied to investigate the stability of the analytes in model water matrix samples under various storage conditions.https://doi.org/10.2478/afpuc-2022-0012biogenic aminesthiaminepyridoxinecapillary zone electrophoresiscyclodextrinsorganic solvents |
| spellingShingle | Matuskova M. Cizmarova I. Chalova P. Stefanik O. Horniakova A. Mikus P. Piestansky J. Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins European Pharmaceutical Journal biogenic amines thiamine pyridoxine capillary zone electrophoresis cyclodextrins organic solvents |
| title | Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| title_full | Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| title_fullStr | Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| title_full_unstemmed | Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| title_short | Influence of Organic Solvents and β-cyclodextrins on Capillary Zone Electrophoresis Separation of Five Biogenic Amines and Two B Vitamins |
| title_sort | influence of organic solvents and β cyclodextrins on capillary zone electrophoresis separation of five biogenic amines and two b vitamins |
| topic | biogenic amines thiamine pyridoxine capillary zone electrophoresis cyclodextrins organic solvents |
| url | https://doi.org/10.2478/afpuc-2022-0012 |
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