Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo
Influenza viruses cause respiratory infections in humans with high morbidity and mortality rates. Neuraminidase inhibitors such as oseltamivir and peramivir are the most commonly used drugs for influenza virus infections. However, the emergence of resistant viruses necessitates the urgent need to de...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biomedicine & Pharmacotherapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222011696 |
| _version_ | 1811180202631364608 |
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| author | Eun-Bin Kwon Young Soo Kim Youn-Hwan Hwang Buyun Kim Sang-Beom Lee Soo Kwon Park Man Soo Choi Hyunil Ha Jang-Gi Choi |
| author_facet | Eun-Bin Kwon Young Soo Kim Youn-Hwan Hwang Buyun Kim Sang-Beom Lee Soo Kwon Park Man Soo Choi Hyunil Ha Jang-Gi Choi |
| author_sort | Eun-Bin Kwon |
| collection | DOAJ |
| description | Influenza viruses cause respiratory infections in humans with high morbidity and mortality rates. Neuraminidase inhibitors such as oseltamivir and peramivir are the most commonly used drugs for influenza virus infections. However, the emergence of resistant viruses necessitates the urgent need to develop next-generation anti-influenza drugs. Soybean (Glycine max L. Merr.) is widely cultivated and used as food worldwide. In addition, soybean has long been used as a nutritional supplement and herbal medicine. However, the potential anti-influenza properties of the soybean cultivar “GL 2626/96″ (SG2626) are yet to be investigated. Herein, we determined whether the ethanolic extract of SG2626 (SG2626E) has anti-viral activity through performing SG2626E pre-, co-, and post-treatment assays, using the influenza green fluorescent protein (GFP)-tagged influenza A/PR/8/34 (A/PR/8/34-GFP) virus. SG2626E showed anti-influenza virus activity in pre- and co-treated cells in a dose-dependent manner, but not in post-treated cells. SG2626E imparted a considerable inhibitory effect on influenza A virus (IAV) infection through blocking viral attachment. SG2626E inhibited the activity of viral hemagglutinin, but not viral neuraminidase of the IAV. SG2626E inhibited IAV infection by reducing intracellular calcium levels in infected human lung epithelial A549 cells. Additionally, SG2626E reduced body weight loss, decreased mortality, and increased the survival rate through reducing viral replication in the lungs of IAV-infected mice. Overall, these results suggest that SG2626E inhibits IAV infection and is a potential novel anti-influenza agent. |
| first_indexed | 2024-04-11T06:46:16Z |
| format | Article |
| id | doaj.art-d25aa21c8c3a4ac187e850fa935edb01 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-11T06:46:16Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-d25aa21c8c3a4ac187e850fa935edb012022-12-22T04:39:20ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-12-01156113780Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivoEun-Bin Kwon0Young Soo Kim1Youn-Hwan Hwang2Buyun Kim3Sang-Beom Lee4Soo Kwon Park5Man Soo Choi6Hyunil Ha7Jang-Gi Choi8Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, South KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, South KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, South KoreaKorean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, South KoreaCrop Foundation Research Division, National Institute of Crop Science (NICS), Wanju 55365, South KoreaCrop Foundation Research Division, National Institute of Crop Science (NICS), Wanju 55365, South KoreaUpland Crop Breeding Research Div. Department of Southern Area Crop Science, National Institute of Crop Science (NICS), Wanju 55365, South KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, South Korea; Corresponding author.Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu 41062, South Korea; Correspondence to: Korean Medicine (KM)-Application Center, Korea Institute of Oriental Medicine, 70, Cheomdanro, Dong-gu, Daegu 41062, South Korea.Influenza viruses cause respiratory infections in humans with high morbidity and mortality rates. Neuraminidase inhibitors such as oseltamivir and peramivir are the most commonly used drugs for influenza virus infections. However, the emergence of resistant viruses necessitates the urgent need to develop next-generation anti-influenza drugs. Soybean (Glycine max L. Merr.) is widely cultivated and used as food worldwide. In addition, soybean has long been used as a nutritional supplement and herbal medicine. However, the potential anti-influenza properties of the soybean cultivar “GL 2626/96″ (SG2626) are yet to be investigated. Herein, we determined whether the ethanolic extract of SG2626 (SG2626E) has anti-viral activity through performing SG2626E pre-, co-, and post-treatment assays, using the influenza green fluorescent protein (GFP)-tagged influenza A/PR/8/34 (A/PR/8/34-GFP) virus. SG2626E showed anti-influenza virus activity in pre- and co-treated cells in a dose-dependent manner, but not in post-treated cells. SG2626E imparted a considerable inhibitory effect on influenza A virus (IAV) infection through blocking viral attachment. SG2626E inhibited the activity of viral hemagglutinin, but not viral neuraminidase of the IAV. SG2626E inhibited IAV infection by reducing intracellular calcium levels in infected human lung epithelial A549 cells. Additionally, SG2626E reduced body weight loss, decreased mortality, and increased the survival rate through reducing viral replication in the lungs of IAV-infected mice. Overall, these results suggest that SG2626E inhibits IAV infection and is a potential novel anti-influenza agent.http://www.sciencedirect.com/science/article/pii/S0753332222011696Glycine maxSoybean GL2626/96Antiviral effectInfluenza A virus |
| spellingShingle | Eun-Bin Kwon Young Soo Kim Youn-Hwan Hwang Buyun Kim Sang-Beom Lee Soo Kwon Park Man Soo Choi Hyunil Ha Jang-Gi Choi Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo Biomedicine & Pharmacotherapy Glycine max Soybean GL2626/96 Antiviral effect Influenza A virus |
| title | Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo |
| title_full | Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo |
| title_fullStr | Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo |
| title_full_unstemmed | Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo |
| title_short | Antiviral activity of soybean GL 2626/96 (Glycine max) ethanolic extract against influenza A virus in vitro and in vivo |
| title_sort | antiviral activity of soybean gl 2626 96 glycine max ethanolic extract against influenza a virus in vitro and in vivo |
| topic | Glycine max Soybean GL2626/96 Antiviral effect Influenza A virus |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222011696 |
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