Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers
Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experiment...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-02-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/9/4/621 |
| _version_ | 1818273057948565504 |
|---|---|
| author | Samira Uharek Sara Baratchi Jiu Yang Zhu Majed Ali Alshehri Arnan Mitchell Karsten Rebner Christian Karnutsch Khashayar Khoshmanesh |
| author_facet | Samira Uharek Sara Baratchi Jiu Yang Zhu Majed Ali Alshehri Arnan Mitchell Karsten Rebner Christian Karnutsch Khashayar Khoshmanesh |
| author_sort | Samira Uharek |
| collection | DOAJ |
| description | Water jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experimental analyses to investigate the thermal characteristics of Petri dish chambers under stable and transient conditions. In particular, we investigated the temperature gradient along the radial axis of the Petri dish under stable conditions, and the transition period under transient conditions. Our studies indicate a radial temperature gradient of 3.3 °C along with a transition period of 27.5 min when increasing the sample temperature from 37 to 45 °C for a standard 35 mm diameter Petri dish. We characterized the temperature gradient and transition period under various operational, geometric, and environmental conditions. Under stable conditions, reducing the diameter of the Petri dish and incorporating a heater underneath the Petri dish can effectively reduce the temperature gradient across the sample. In comparison, under transient conditions, reducing the diameter of the Petri dish, reducing sample volume, and using glass Petri dish chambers can reduce the transition period. |
| first_indexed | 2024-12-12T21:51:55Z |
| format | Article |
| id | doaj.art-d25d2f53a80e4511b279acfc115396d0 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-12T21:51:55Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-d25d2f53a80e4511b279acfc115396d02022-12-22T00:10:46ZengMDPI AGApplied Sciences2076-34172019-02-019462110.3390/app9040621app9040621Water Jacket Systems for Temperature Control of Petri Dish Cell Culture ChambersSamira Uharek0Sara Baratchi1Jiu Yang Zhu2Majed Ali Alshehri3Arnan Mitchell4Karsten Rebner5Christian Karnutsch6Khashayar Khoshmanesh7Institute for Sensor- and Information Systems (ISIS), Research Group ‘Integrated Optofluidics and Nanophotonics (IONAS)’, University of Applied Sciences Karlsruhe, 76133 Karlsruhe, GermanySchool of Health and Biomedical Sciences, RMIT University, Melbourne, VIC 3083, AustraliaSchool of Engineering, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Engineering, RMIT University, Melbourne, VIC 3001, AustraliaSchool of Engineering, RMIT University, Melbourne, VIC 3001, AustraliaDepartment of Applied Chemistry, Reutlingen University, Reutlingen, 72762 Baden-Wuerttemberg, GermanyInstitute for Sensor- and Information Systems (ISIS), Research Group ‘Integrated Optofluidics and Nanophotonics (IONAS)’, University of Applied Sciences Karlsruhe, 76133 Karlsruhe, GermanySchool of Engineering, RMIT University, Melbourne, VIC 3001, AustraliaWater jacket systems are routinely used to control the temperature of Petri dish cell culture chambers. Despite their widespread use, the thermal characteristics of such systems have not been fully investigated. In this study, we conducted a comprehensive set of theoretical, numerical and experimental analyses to investigate the thermal characteristics of Petri dish chambers under stable and transient conditions. In particular, we investigated the temperature gradient along the radial axis of the Petri dish under stable conditions, and the transition period under transient conditions. Our studies indicate a radial temperature gradient of 3.3 °C along with a transition period of 27.5 min when increasing the sample temperature from 37 to 45 °C for a standard 35 mm diameter Petri dish. We characterized the temperature gradient and transition period under various operational, geometric, and environmental conditions. Under stable conditions, reducing the diameter of the Petri dish and incorporating a heater underneath the Petri dish can effectively reduce the temperature gradient across the sample. In comparison, under transient conditions, reducing the diameter of the Petri dish, reducing sample volume, and using glass Petri dish chambers can reduce the transition period.https://www.mdpi.com/2076-3417/9/4/621temperature controlwater jacketconvective heatersPetri dish |
| spellingShingle | Samira Uharek Sara Baratchi Jiu Yang Zhu Majed Ali Alshehri Arnan Mitchell Karsten Rebner Christian Karnutsch Khashayar Khoshmanesh Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers Applied Sciences temperature control water jacket convective heaters Petri dish |
| title | Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers |
| title_full | Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers |
| title_fullStr | Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers |
| title_full_unstemmed | Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers |
| title_short | Water Jacket Systems for Temperature Control of Petri Dish Cell Culture Chambers |
| title_sort | water jacket systems for temperature control of petri dish cell culture chambers |
| topic | temperature control water jacket convective heaters Petri dish |
| url | https://www.mdpi.com/2076-3417/9/4/621 |
| work_keys_str_mv | AT samirauharek waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT sarabaratchi waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT jiuyangzhu waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT majedalialshehri waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT arnanmitchell waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT karstenrebner waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT christiankarnutsch waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers AT khashayarkhoshmanesh waterjacketsystemsfortemperaturecontrolofpetridishcellculturechambers |