Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis
Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes oblitera...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2021-08-01
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| Series: | Notulae Scientia Biologicae |
| Subjects: | |
| Online Access: | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995 |
| _version_ | 1818001497785368576 |
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| author | Wei-Seng HO Wee-Hiang ENG Kwong-Hung LING |
| author_facet | Wei-Seng HO Wee-Hiang ENG Kwong-Hung LING |
| author_sort | Wei-Seng HO |
| collection | DOAJ |
| description | Chromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count. |
| first_indexed | 2024-04-14T03:35:02Z |
| format | Article |
| id | doaj.art-d26426ca2bb14ee88945fb17b578b43f |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T03:35:02Z |
| publishDate | 2021-08-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-d26426ca2bb14ee88945fb17b578b43f2022-12-22T02:14:49ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642021-08-0113310.15835/nsb13310995Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesisWei-Seng HO0Wee-Hiang ENG1Kwong-Hung LING2Universiti Malaysia Sarawak, Forest Genomics and Informatics Laboratory (fGiLab), Faculty of Resource Science and Technology, 94300 Kota Samarahan, SarawakUniversiti Malaysia Sarawak, Forest Genomics and Informatics Laboratory (fGiLab), Faculty of Resource Science and Technology, 94300 Kota Samarahan, SarawakSarawak Timber Association, Wisma STA, 26, Jalan Datuk Abang Abdul Rahim, 93450 Kuching, SarawakChromosome count is the only direct way to determine the number of chromosomes of a species. This study is often considered trivial that seldom described and discussed in detail. Therefore, it is inevitable that the chromosome count protocol should be revised and revisited before it becomes obliterated. In the present study, we encountered challenges in obtaining a clear micrograph for the chromosome count of active mitotic cells of Neolamarckia cadamba (Roxb.) Bosser (Rubiaceae) root tips. Several obstacles were determined through micrograph observation, such as existing unwanted particles in cells, poor chromosome staining and chromosome clumping. To overcome these, root tip types, staining methodologies, squashing methods were among the factors assessed to obtain clear micrographs. The chromosome counts of N. cadamba under optimized procedure showed 2n = 44 chromosomes. We also apply digital technology in chromosome counts, such as online databases and graphic software that are open source and freely accessible to the public. Only basic laboratory equipment and chemicals were used throughout the study, thus making this study economical and applicable in a basic laboratory. The availability of online digital software and databases provide open-source platforms that will ease the efforts in chromosome count.https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995ImageJmitotic chromosomespolyploidRubiaceaesingle and double staining |
| spellingShingle | Wei-Seng HO Wee-Hiang ENG Kwong-Hung LING Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis Notulae Scientia Biologicae ImageJ mitotic chromosomes polyploid Rubiaceae single and double staining |
| title | Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
| title_full | Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
| title_fullStr | Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
| title_full_unstemmed | Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
| title_short | Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis |
| title_sort | cytogenetic chromosome count optimization and automation of neolamarckia cadamba rubiaceae root tips derived from in vitro mutagenesis |
| topic | ImageJ mitotic chromosomes polyploid Rubiaceae single and double staining |
| url | https://www.notulaebiologicae.ro/index.php/nsb/article/view/10995 |
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