Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria
Oxidized multi-walled carbon nanotubes (oxCNTs) were functionalized by a simple non-covalent modification procedure using quaternized hyperbranched poly(ethyleneimine) derivatives (QPEIs), with various quaternization degrees. Structural characterization of these hybrids using a variety of techniques...
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MDPI AG
2020-10-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/10/293 |
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| author | Nikolaos S. Heliopoulos Georgia Kythreoti Kyriaki Marina Lyra Katerina N. Panagiotaki Aggeliki Papavasiliou Elias Sakellis Sergios Papageorgiou Antonios Kouloumpis Dimitrios Gournis Fotios K. Katsaros Kostas Stamatakis Zili Sideratou |
| author_facet | Nikolaos S. Heliopoulos Georgia Kythreoti Kyriaki Marina Lyra Katerina N. Panagiotaki Aggeliki Papavasiliou Elias Sakellis Sergios Papageorgiou Antonios Kouloumpis Dimitrios Gournis Fotios K. Katsaros Kostas Stamatakis Zili Sideratou |
| author_sort | Nikolaos S. Heliopoulos |
| collection | DOAJ |
| description | Oxidized multi-walled carbon nanotubes (oxCNTs) were functionalized by a simple non-covalent modification procedure using quaternized hyperbranched poly(ethyleneimine) derivatives (QPEIs), with various quaternization degrees. Structural characterization of these hybrids using a variety of techniques, revealed the successful and homogenous anchoring of QPEIs on the oxCNTs’ surface. Moreover, these hybrids efficiently dispersed in aqueous media, forming dispersions with excellent aqueous stability for over 12 months. Their cytotoxicity effect was investigated on two types of gram(−) bacteria, an autotrophic (cyanobacterium <i>Synechococcus</i> sp. PCC 7942) and a heterotrophic (bacterium <i>Escherichia coli</i>). An enhanced, dose-dependent antibacterial and anti-cyanobacterial activity against both tested organisms was observed, increasing with the quaternization degree. Remarkably, in the photosynthetic bacteria it was shown that the hybrid materials affect their photosynthetic apparatus by selective inhibition of the Photosystem-I electron transport activity. Cytotoxicity studies on a human prostate carcinoma DU145 cell line and 3T3 mouse fibroblasts revealed that all hybrids exhibit high cytocompatibility in the concentration range, in which they also exhibit both high antibacterial and anti-cyanobacterial activity. Thus, QPEI-functionalized oxCNTs can be very attractive candidates as antibacterial and anti-cyanobacterial agents that can be used for potential applications in the disinfection industry, as well as for the control of harmful cyanobacterial blooms. |
| first_indexed | 2024-03-10T15:50:11Z |
| format | Article |
| id | doaj.art-d26602948f09481ca27a05ab82d7bc04 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T15:50:11Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-d26602948f09481ca27a05ab82d7bc042023-11-20T16:10:18ZengMDPI AGPharmaceuticals1424-82472020-10-01131029310.3390/ph13100293Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative BacteriaNikolaos S. Heliopoulos0Georgia Kythreoti1Kyriaki Marina Lyra2Katerina N. Panagiotaki3Aggeliki Papavasiliou4Elias Sakellis5Sergios Papageorgiou6Antonios Kouloumpis7Dimitrios Gournis8Fotios K. Katsaros9Kostas Stamatakis10Zili Sideratou11Institute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceDepartment of Material Science & Engineering, University of Ioannina, 45110 Ioannina, GreeceDepartment of Material Science & Engineering, University of Ioannina, 45110 Ioannina, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Biosciences and Applications, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceInstitute of Nanoscience and Nanotechnology, National Centre of Scientific Research ‘‘Demokritos”, 15310 Aghia Paraskevi, GreeceOxidized multi-walled carbon nanotubes (oxCNTs) were functionalized by a simple non-covalent modification procedure using quaternized hyperbranched poly(ethyleneimine) derivatives (QPEIs), with various quaternization degrees. Structural characterization of these hybrids using a variety of techniques, revealed the successful and homogenous anchoring of QPEIs on the oxCNTs’ surface. Moreover, these hybrids efficiently dispersed in aqueous media, forming dispersions with excellent aqueous stability for over 12 months. Their cytotoxicity effect was investigated on two types of gram(−) bacteria, an autotrophic (cyanobacterium <i>Synechococcus</i> sp. PCC 7942) and a heterotrophic (bacterium <i>Escherichia coli</i>). An enhanced, dose-dependent antibacterial and anti-cyanobacterial activity against both tested organisms was observed, increasing with the quaternization degree. Remarkably, in the photosynthetic bacteria it was shown that the hybrid materials affect their photosynthetic apparatus by selective inhibition of the Photosystem-I electron transport activity. Cytotoxicity studies on a human prostate carcinoma DU145 cell line and 3T3 mouse fibroblasts revealed that all hybrids exhibit high cytocompatibility in the concentration range, in which they also exhibit both high antibacterial and anti-cyanobacterial activity. Thus, QPEI-functionalized oxCNTs can be very attractive candidates as antibacterial and anti-cyanobacterial agents that can be used for potential applications in the disinfection industry, as well as for the control of harmful cyanobacterial blooms.https://www.mdpi.com/1424-8247/13/10/293carbon nanotubesquaternary ammonium groupshyperbranched dendritic polymersantibacterial propertiesanti-cyanobacterial properties |
| spellingShingle | Nikolaos S. Heliopoulos Georgia Kythreoti Kyriaki Marina Lyra Katerina N. Panagiotaki Aggeliki Papavasiliou Elias Sakellis Sergios Papageorgiou Antonios Kouloumpis Dimitrios Gournis Fotios K. Katsaros Kostas Stamatakis Zili Sideratou Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria Pharmaceuticals carbon nanotubes quaternary ammonium groups hyperbranched dendritic polymers antibacterial properties anti-cyanobacterial properties |
| title | Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria |
| title_full | Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria |
| title_fullStr | Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria |
| title_full_unstemmed | Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria |
| title_short | Cytotoxicity Effects of Water-Soluble Multi-Walled Carbon Nanotubes Decorated with Quaternized Hyperbranched Poly(ethyleneimine) Derivatives on Autotrophic and Heterotrophic Gram-Negative Bacteria |
| title_sort | cytotoxicity effects of water soluble multi walled carbon nanotubes decorated with quaternized hyperbranched poly ethyleneimine derivatives on autotrophic and heterotrophic gram negative bacteria |
| topic | carbon nanotubes quaternary ammonium groups hyperbranched dendritic polymers antibacterial properties anti-cyanobacterial properties |
| url | https://www.mdpi.com/1424-8247/13/10/293 |
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