In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens
The stingless bee, Tetragonula sapiens, is one of the species producing propolis in Indonesia. To date, there is still limited information about the standard characteristics and biological activity of propolis. This study investigated the standardized extract of Indonesian propolis in terms of its p...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723002008 |
| _version_ | 1797774843784986624 |
|---|---|
| author | Siti Farida Diah Kartika Pratami Muhamad Sahlan Abdul Mun'im Ratna Djamil Wiwi Winarti Rashid Ayub Tahani Awad Alahmadi Siti Irma Rahmawati Masteria Yunovilsa Putra Asep Bayu Muhammad Iqbal |
| author_facet | Siti Farida Diah Kartika Pratami Muhamad Sahlan Abdul Mun'im Ratna Djamil Wiwi Winarti Rashid Ayub Tahani Awad Alahmadi Siti Irma Rahmawati Masteria Yunovilsa Putra Asep Bayu Muhammad Iqbal |
| author_sort | Siti Farida |
| collection | DOAJ |
| description | The stingless bee, Tetragonula sapiens, is one of the species producing propolis in Indonesia. To date, there is still limited information about the standard characteristics and biological activity of propolis. This study investigated the standardized extract of Indonesian propolis in terms of its potency for controlling diabetes and high blood pressure in vitro, in accordance with its standard parameters. Three types of extracts; i.e., ethanolic extract of propolis (EEP), spray-dry propolis without wax (SDPNW), and spray-dry propolis with wax (SDPW); were prepared from T. sapiens stingless bees collected in South Sulawesi, Indonesia. The ethanolic extractable, loss on drying, ash, and water content parameters of EEP, SDPNW, and SDPW met the natural extract requirement. There is no significant value for their residual solvent levels (<0.54%), heavy metals (the absence of As, Cd, Pb, and Hg contamination), or the absence of microbial contamination. ACE inhibition activity on captopril, EEP, SDPNW, and SDPW had IC50 values of 5.97, 115.06, 112.93, and 116.70 ppm, respectively. While the IC50 values of acarbose, SDPNW, EEP, and SDPW for antidiabetic activity of α-glucosidase inhibition were 53.34, 56.48, 60.09, and 68.28 ppm, respectively. The TFC and TPC content of SDPNW were higher than EEP and SDPW, with values of 1.59% and 1.93%, respectively. It can be said that Indonesian propolis extract meets the standards for an extract and has the potential to reduce diabetic and hypertensive diseases. Further preclinical and clinical studies are needed to develop standardized propolis extract as an anti-diabetic and antihypertensive agent. |
| first_indexed | 2024-03-12T22:27:07Z |
| format | Article |
| id | doaj.art-d2666286fe784e578329b0653a04ca52 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-12T22:27:07Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-d2666286fe784e578329b0653a04ca522023-07-22T04:51:29ZengElsevierJournal of King Saud University: Science1018-36472023-08-01356102738In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiensSiti Farida0Diah Kartika Pratami1Muhamad Sahlan2Abdul Mun'im3Ratna Djamil4Wiwi Winarti5Rashid Ayub6Tahani Awad Alahmadi7Siti Irma Rahmawati8Masteria Yunovilsa Putra9Asep Bayu10Muhammad Iqbal11Department of Medical Pharmacy, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia; Faculty of Medicine, Universitas Sultan Ageng Tirtayasa, Cilegon, Banten 42434, Indonesia; Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java 16424, IndonesiaFaculty of Pharmacy, Universitas Indonesia, Cluster of Health Sciences Building, Depok 16424, West Java, Indonesia; National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok, West Java 16424, Indonesia; Center for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta 12640, Indonesia; Corresponding author at: Faculty of Pharmacy, Universitas Indonesia, Cluster of Health Sciences Building, Depok 16424, West Java, Indonesia.Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java 16424, Indonesia; Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java 16424, IndonesiaFaculty of Pharmacy, Universitas Indonesia, Cluster of Health Sciences Building, Depok 16424, West Java, Indonesia; National Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok, West Java 16424, IndonesiaCenter for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta 12640, Indonesia; Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta, 12640, IndonesiaCenter for Study of Natural Product for Degenerative Disease, Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta 12640, Indonesia; Faculty of Pharmacy, Pancasila University, South Jakarta, DKI Jakarta, 12640, IndonesiaThe National Plan for Science and Technology, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pediatrics, College of Medicine and King Khalid University Hospital, King Saud University, Medical City, PO Box-2925, Riyadh 11461, Saudi ArabiaNational Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok, West Java 16424, Indonesia; Research Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor KM 46 Cibinong, Bogor, West Java 16911, IndonesiaNational Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok, West Java 16424, Indonesia; Research Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor KM 46 Cibinong, Bogor, West Java 16911, IndonesiaNational Metabolomics Collaborative Research Center, Faculty of Pharmacy, Universitas Indonesia, Kampus UI, Depok, West Java 16424, Indonesia; Research Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta-Bogor KM 46 Cibinong, Bogor, West Java 16911, IndonesiaDepartment of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, United KingdomThe stingless bee, Tetragonula sapiens, is one of the species producing propolis in Indonesia. To date, there is still limited information about the standard characteristics and biological activity of propolis. This study investigated the standardized extract of Indonesian propolis in terms of its potency for controlling diabetes and high blood pressure in vitro, in accordance with its standard parameters. Three types of extracts; i.e., ethanolic extract of propolis (EEP), spray-dry propolis without wax (SDPNW), and spray-dry propolis with wax (SDPW); were prepared from T. sapiens stingless bees collected in South Sulawesi, Indonesia. The ethanolic extractable, loss on drying, ash, and water content parameters of EEP, SDPNW, and SDPW met the natural extract requirement. There is no significant value for their residual solvent levels (<0.54%), heavy metals (the absence of As, Cd, Pb, and Hg contamination), or the absence of microbial contamination. ACE inhibition activity on captopril, EEP, SDPNW, and SDPW had IC50 values of 5.97, 115.06, 112.93, and 116.70 ppm, respectively. While the IC50 values of acarbose, SDPNW, EEP, and SDPW for antidiabetic activity of α-glucosidase inhibition were 53.34, 56.48, 60.09, and 68.28 ppm, respectively. The TFC and TPC content of SDPNW were higher than EEP and SDPW, with values of 1.59% and 1.93%, respectively. It can be said that Indonesian propolis extract meets the standards for an extract and has the potential to reduce diabetic and hypertensive diseases. Further preclinical and clinical studies are needed to develop standardized propolis extract as an anti-diabetic and antihypertensive agent.http://www.sciencedirect.com/science/article/pii/S1018364723002008α-Glucosidase enzymeAngiotensin converting enzymeAntidiabeticAntihypertensivePropolisTetragonula sapiens |
| spellingShingle | Siti Farida Diah Kartika Pratami Muhamad Sahlan Abdul Mun'im Ratna Djamil Wiwi Winarti Rashid Ayub Tahani Awad Alahmadi Siti Irma Rahmawati Masteria Yunovilsa Putra Asep Bayu Muhammad Iqbal In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens Journal of King Saud University: Science α-Glucosidase enzyme Angiotensin converting enzyme Antidiabetic Antihypertensive Propolis Tetragonula sapiens |
| title | In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens |
| title_full | In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens |
| title_fullStr | In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens |
| title_full_unstemmed | In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens |
| title_short | In vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of Indonesian stingless bee Tetragonula sapiens |
| title_sort | in vitro study on antidiabetic and antihypertensive activities of ethanolic extract of propolis of indonesian stingless bee tetragonula sapiens |
| topic | α-Glucosidase enzyme Angiotensin converting enzyme Antidiabetic Antihypertensive Propolis Tetragonula sapiens |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723002008 |
| work_keys_str_mv | AT sitifarida invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT diahkartikapratami invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT muhamadsahlan invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT abdulmunim invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT ratnadjamil invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT wiwiwinarti invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT rashidayub invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT tahaniawadalahmadi invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT sitiirmarahmawati invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT masteriayunovilsaputra invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT asepbayu invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens AT muhammadiqbal invitrostudyonantidiabeticandantihypertensiveactivitiesofethanolicextractofpropolisofindonesianstinglessbeetetragonulasapiens |