Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy
Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy fo...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Simona Dzurendová Volha Shapaval Valeria Tafintseva Achim Kohler Dana Byrtusová Martin Szotkowski Ivana Márová Boris Zimmermann |
| author_facet | Simona Dzurendová Volha Shapaval Valeria Tafintseva Achim Kohler Dana Byrtusová Martin Szotkowski Ivana Márová Boris Zimmermann |
| author_sort | Simona Dzurendová |
| collection | DOAJ |
| description | Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83–0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents. |
| first_indexed | 2024-03-10T10:08:54Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:08:54Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d2672859b6e04778a54850bf7e8407082023-11-22T01:19:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213671010.3390/ijms22136710Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman SpectroscopySimona Dzurendová0Volha Shapaval1Valeria Tafintseva2Achim Kohler3Dana Byrtusová4Martin Szotkowski5Ivana Márová6Boris Zimmermann7Faculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 61200 Brno, Czech RepublicFaculty of Chemistry, Brno University of Technology, Purkyňova 464/118, 61200 Brno, Czech RepublicFaculty of Science and Technology, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Ås, NorwayOleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83–0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents.https://www.mdpi.com/1422-0067/22/13/6710oleaginous microorganismsbiodieselpigmentsbiopolymerscarotenoidsfatty acids |
| spellingShingle | Simona Dzurendová Volha Shapaval Valeria Tafintseva Achim Kohler Dana Byrtusová Martin Szotkowski Ivana Márová Boris Zimmermann Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy International Journal of Molecular Sciences oleaginous microorganisms biodiesel pigments biopolymers carotenoids fatty acids |
| title | Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy |
| title_full | Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy |
| title_fullStr | Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy |
| title_full_unstemmed | Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy |
| title_short | Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy |
| title_sort | assessment of biotechnologically important filamentous fungal biomass by fourier transform raman spectroscopy |
| topic | oleaginous microorganisms biodiesel pigments biopolymers carotenoids fatty acids |
| url | https://www.mdpi.com/1422-0067/22/13/6710 |
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