Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci
Abstract Albeit the majority of eukaryotic genomes can be pervasively transcribed to a diverse population of lncRNAs and various subtypes of lncRNA are discovered. However, the genome‐wide study of miRNA‐derived lncRNAs is still lacking. Here, it is reported that over 800 miRNA gene‐originated lncRN...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-11-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202203987 |
| _version_ | 1797465057928413184 |
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| author | Weiqian Li Yue Huo Yue Ren Chenxi Han Shuo Li Kangning Wang Manman He Yiying Chen Yanran Wang Lingjie Xu Yuehong Guo Yanmin Si Yufeng Gao Jiayue Xu Xiaoshuang Wang Yanni Ma Jia Yu Fang Wang |
| author_facet | Weiqian Li Yue Huo Yue Ren Chenxi Han Shuo Li Kangning Wang Manman He Yiying Chen Yanran Wang Lingjie Xu Yuehong Guo Yanmin Si Yufeng Gao Jiayue Xu Xiaoshuang Wang Yanni Ma Jia Yu Fang Wang |
| author_sort | Weiqian Li |
| collection | DOAJ |
| description | Abstract Albeit the majority of eukaryotic genomes can be pervasively transcribed to a diverse population of lncRNAs and various subtypes of lncRNA are discovered. However, the genome‐wide study of miRNA‐derived lncRNAs is still lacking. Here, it is reported that over 800 miRNA gene‐originated lncRNAs (molncRNAs) are generated from miRNA loci. One of them, molnc‐301b from miR‐301b and miR‐130b, functions as an “RNA decoy” to facilitate dissociation of the chromatin remodeling protein SMARCA5 from chromatin and thereby sequester transcription and mRNA translation. Specifically, molnc‐301b attenuates erythropoiesis by mitigating the transcription of erythropoietic and translation‐associated genes, such as GATA1 and FOS. In addition, a useful and powerful CRISPR screen platform to characterize the biological functions of molncRNAs at large‐scale and single‐cell levels is established and 29 functional molncRNAs in hematopoietic cells are identified. Collectively, the focus is on miRNA‐derived lncRNAs, deciphering their landscape during normal hematopoiesis, and comprehensively evaluating their potential roles. |
| first_indexed | 2024-03-09T18:17:04Z |
| format | Article |
| id | doaj.art-d26c16f6a61d4156bec2f1315dd36454 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-09T18:17:04Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-d26c16f6a61d4156bec2f1315dd364542023-11-24T08:40:29ZengWileyAdvanced Science2198-38442023-11-011033n/an/a10.1002/advs.202203987Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA LociWeiqian Li0Yue Huo1Yue Ren2Chenxi Han3Shuo Li4Kangning Wang5Manman He6Yiying Chen7Yanran Wang8Lingjie Xu9Yuehong Guo10Yanmin Si11Yufeng Gao12Jiayue Xu13Xiaoshuang Wang14Yanni Ma15Jia Yu16Fang Wang17State Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaEmergency Department of West China Hospital Sichuan University Chengdu 610041 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaState Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical Sciences Haihe Laboratory of Cell Ecosystem The Key Laboratory of RNA and Hematopoietic Regulation Chinese Academy of Medical Sciences / Peking Union Medical College Beijing 100005 P.R. ChinaAbstract Albeit the majority of eukaryotic genomes can be pervasively transcribed to a diverse population of lncRNAs and various subtypes of lncRNA are discovered. However, the genome‐wide study of miRNA‐derived lncRNAs is still lacking. Here, it is reported that over 800 miRNA gene‐originated lncRNAs (molncRNAs) are generated from miRNA loci. One of them, molnc‐301b from miR‐301b and miR‐130b, functions as an “RNA decoy” to facilitate dissociation of the chromatin remodeling protein SMARCA5 from chromatin and thereby sequester transcription and mRNA translation. Specifically, molnc‐301b attenuates erythropoiesis by mitigating the transcription of erythropoietic and translation‐associated genes, such as GATA1 and FOS. In addition, a useful and powerful CRISPR screen platform to characterize the biological functions of molncRNAs at large‐scale and single‐cell levels is established and 29 functional molncRNAs in hematopoietic cells are identified. Collectively, the focus is on miRNA‐derived lncRNAs, deciphering their landscape during normal hematopoiesis, and comprehensively evaluating their potential roles.https://doi.org/10.1002/advs.202203987CRISPR screenmiR‐301bmiRNA gene‐originated lncRNAs (molncRNAs)SMARCA5 |
| spellingShingle | Weiqian Li Yue Huo Yue Ren Chenxi Han Shuo Li Kangning Wang Manman He Yiying Chen Yanran Wang Lingjie Xu Yuehong Guo Yanmin Si Yufeng Gao Jiayue Xu Xiaoshuang Wang Yanni Ma Jia Yu Fang Wang Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci Advanced Science CRISPR screen miR‐301b miRNA gene‐originated lncRNAs (molncRNAs) SMARCA5 |
| title | Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci |
| title_full | Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci |
| title_fullStr | Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci |
| title_full_unstemmed | Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci |
| title_short | Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci |
| title_sort | deciphering the functional long non coding rnas derived from microrna loci |
| topic | CRISPR screen miR‐301b miRNA gene‐originated lncRNAs (molncRNAs) SMARCA5 |
| url | https://doi.org/10.1002/advs.202203987 |
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