Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model
Purpose: Chronic prostatitis (CP), including chronic pelvic pain syndrome (CPPS), is the most commonly encountered manifestation of prostatitis. The aim of this study was to evaluate the effect of electric stimulation hyperthermia treatment (ESHT) on CP/CPPS and to explore the underlying mechanism...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Sexual Medicine and Andrology
2020-07-01
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| Series: | The World Journal of Men's Health |
| Subjects: |
| _version_ | 1818879888281567232 |
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| author | Guan Qun Zhu Seung Hwan Jeon Kyu Won Lee Wen Jie Tian Hyuk Jin Cho U-Syn Ha Sung Hoo Hong Ji Youl Lee Myeong Keon Moon Sung Hee Moon Sae Woong Kim Woong Jin Bae |
| author_facet | Guan Qun Zhu Seung Hwan Jeon Kyu Won Lee Wen Jie Tian Hyuk Jin Cho U-Syn Ha Sung Hoo Hong Ji Youl Lee Myeong Keon Moon Sung Hee Moon Sae Woong Kim Woong Jin Bae |
| author_sort | Guan Qun Zhu |
| collection | DOAJ |
| description | Purpose: Chronic prostatitis (CP), including chronic pelvic pain syndrome (CPPS), is the most commonly encountered manifestation
of prostatitis. The aim of this study was to evaluate the effect of electric stimulation hyperthermia treatment (ESHT)
on CP/CPPS and to explore the underlying mechanism.
Materials and Methods: RWPE-2 cells with lipopolysaccharide-induced inflammation and a prostatitis rat model induced
by 17β-estradiol and dihydrotestosterone underwent sham, electric stimulation, or ESHT treatment. Four weeks later, cells,
supernatants, and rat prostates were collected for analysis using immunohistochemistry, Western blots, and enzyme-linked
immunosorbent assays.
Results: We found that ESHT improved prostatitis in vivo and attenuated inflammation in vitro. ESHT significantly induced
suppressor of cytokine signaling 3 (SOCS3) expression and subsequently promoted HSP70. It attenuated inflammation
through decreased expression of toll-like receptor 4 (TLR4), nuclear factor kappa B, and subsequent inflammatory cytokines.
ESHT also inhibited apoptosis and released growth factor in tissue affected by prostatitis.
Conclusions: ESHT improved CP/CPPS and reversed pathologic changes of prostatitis by inhibiting the SOCS3-TLR4 pathway. |
| first_indexed | 2024-12-19T14:37:14Z |
| format | Article |
| id | doaj.art-d26fad0744d84a288ba18d8fe91b72f3 |
| institution | Directory Open Access Journal |
| issn | 2287-4208 2287-4690 |
| language | English |
| last_indexed | 2024-12-19T14:37:14Z |
| publishDate | 2020-07-01 |
| publisher | Korean Society for Sexual Medicine and Andrology |
| record_format | Article |
| series | The World Journal of Men's Health |
| spelling | doaj.art-d26fad0744d84a288ba18d8fe91b72f32022-12-21T20:17:12ZengKorean Society for Sexual Medicine and AndrologyThe World Journal of Men's Health2287-42082287-46902020-07-0138335936910.5534/wjmh.190078Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat ModelGuan Qun Zhu0https://orcid.org/0000-0001-7841-8026Seung Hwan Jeon1https://orcid.org/0000-0001-6695-6097Kyu Won Lee2https://orcid.org/0000-0001-7308-1583Wen Jie Tian3https://orcid.org/0000-0002-6037-1319Hyuk Jin Cho4https://orcid.org/0000-0003-2599-2950U-Syn Ha5https://orcid.org/0000-0001-9930-0588Sung Hoo Hong6https://orcid.org/0000-0002-1952-4010Ji Youl Lee7https://orcid.org/0000-0001-6775-1157Myeong Keon Moon8https://orcid.org/0000-0002-4461-371XSung Hee Moon9https://orcid.org/0000-0002-5310-0131Sae Woong Kim10https://orcid.org/0000-0002-9127-9400Woong Jin Bae11https://orcid.org/0000-0001-7703-1161The Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaThe Catholic University of KoreaBuheung MedicalBuheung MedicalThe Catholic University of KoreaThe Catholic University of KoreaPurpose: Chronic prostatitis (CP), including chronic pelvic pain syndrome (CPPS), is the most commonly encountered manifestation of prostatitis. The aim of this study was to evaluate the effect of electric stimulation hyperthermia treatment (ESHT) on CP/CPPS and to explore the underlying mechanism. Materials and Methods: RWPE-2 cells with lipopolysaccharide-induced inflammation and a prostatitis rat model induced by 17β-estradiol and dihydrotestosterone underwent sham, electric stimulation, or ESHT treatment. Four weeks later, cells, supernatants, and rat prostates were collected for analysis using immunohistochemistry, Western blots, and enzyme-linked immunosorbent assays. Results: We found that ESHT improved prostatitis in vivo and attenuated inflammation in vitro. ESHT significantly induced suppressor of cytokine signaling 3 (SOCS3) expression and subsequently promoted HSP70. It attenuated inflammation through decreased expression of toll-like receptor 4 (TLR4), nuclear factor kappa B, and subsequent inflammatory cytokines. ESHT also inhibited apoptosis and released growth factor in tissue affected by prostatitis. Conclusions: ESHT improved CP/CPPS and reversed pathologic changes of prostatitis by inhibiting the SOCS3-TLR4 pathway.electric stimulation hyperthermia treatmentneuroinflammationprostatitissuppressor of cytokine signaling 3-toll like receptor 4 pathway |
| spellingShingle | Guan Qun Zhu Seung Hwan Jeon Kyu Won Lee Wen Jie Tian Hyuk Jin Cho U-Syn Ha Sung Hoo Hong Ji Youl Lee Myeong Keon Moon Sung Hee Moon Sae Woong Kim Woong Jin Bae Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model The World Journal of Men's Health electric stimulation hyperthermia treatment neuroinflammation prostatitis suppressor of cytokine signaling 3-toll like receptor 4 pathway |
| title | Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model |
| title_full | Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model |
| title_fullStr | Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model |
| title_full_unstemmed | Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model |
| title_short | Electric Stimulation Hyperthermia Relieves Inflammation via the Suppressor of Cytokine Signaling 3-Toll Like Receptor 4 Pathway in a Prostatitis Rat Model |
| title_sort | electric stimulation hyperthermia relieves inflammation via the suppressor of cytokine signaling 3 toll like receptor 4 pathway in a prostatitis rat model |
| topic | electric stimulation hyperthermia treatment neuroinflammation prostatitis suppressor of cytokine signaling 3-toll like receptor 4 pathway |
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