Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis
Background: Tuberculosis (TB) remains a serious public health problem worldwide. The emergence of drug resistance and multidrug resistance (MDR) has become the main threat to TB treatment and control programs. Rapid detection is critical for the effective treatment of patients. In recent times, a ne...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Global Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.jgid.org/article.asp?issn=0974-777X;year=2014;volume=6;issue=4;spage=151;epage=156;aulast=Patil |
| _version_ | 1819262185265692672 |
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| author | Santosh S Patil Shivajirao T Mohite Sunanda A Kulkarni Usha S Udgaonkar |
| author_facet | Santosh S Patil Shivajirao T Mohite Sunanda A Kulkarni Usha S Udgaonkar |
| author_sort | Santosh S Patil |
| collection | DOAJ |
| description | Background: Tuberculosis (TB) remains a serious public health problem worldwide. The emergence of drug resistance and multidrug resistance (MDR) has become the main threat to TB treatment and control programs. Rapid detection is critical for the effective treatment of patients. In recent times, a new method using the colorimetric indicator resazurin has been proposed for drug susceptibility of Mycobacterium tuberculosis. Materials and Methods: In this study, the resazurin reduction assay was adapted to screw cap tubes. Using the Resazurin Tube Method (RTM), a total of 100 clinical isolates were tested against Rifampicin (RIF) and Isoniazide (INH). By visual reading, the minimum inhibitory concentrations (MICs) were obtained after eight days. The results obtained were compared with the gold standard proportion method. Results: Excellent results were obtained for RTM with a sensitivity of 100% for both RIF and INH, with a specificity of 98.7 and 95.3%, respectively. Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively. Conclusion: The RTM appears to be a reliable method for the rapid and simultaneous detection of MDR-TB and drug susceptibility testing (DST) of M. tuberculosis. It is simple, inexpensive, and with no biohazard risk involved. |
| first_indexed | 2024-12-23T19:53:40Z |
| format | Article |
| id | doaj.art-d270d3c52ad64b82ad2d32eb6befd29a |
| institution | Directory Open Access Journal |
| issn | 0974-777X |
| language | English |
| last_indexed | 2024-12-23T19:53:40Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Global Infectious Diseases |
| spelling | doaj.art-d270d3c52ad64b82ad2d32eb6befd29a2022-12-21T17:33:18ZengWolters Kluwer Medknow PublicationsJournal of Global Infectious Diseases0974-777X2014-01-016415115610.4103/0974-777X.145239Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium TuberculosisSantosh S PatilShivajirao T MohiteSunanda A KulkarniUsha S UdgaonkarBackground: Tuberculosis (TB) remains a serious public health problem worldwide. The emergence of drug resistance and multidrug resistance (MDR) has become the main threat to TB treatment and control programs. Rapid detection is critical for the effective treatment of patients. In recent times, a new method using the colorimetric indicator resazurin has been proposed for drug susceptibility of Mycobacterium tuberculosis. Materials and Methods: In this study, the resazurin reduction assay was adapted to screw cap tubes. Using the Resazurin Tube Method (RTM), a total of 100 clinical isolates were tested against Rifampicin (RIF) and Isoniazide (INH). By visual reading, the minimum inhibitory concentrations (MICs) were obtained after eight days. The results obtained were compared with the gold standard proportion method. Results: Excellent results were obtained for RTM with a sensitivity of 100% for both RIF and INH, with a specificity of 98.7 and 95.3%, respectively. Kappa is the measure of agreement between the RTM and proportion method (PM) for RIF and INH, which was found to be 0.972 and 0.935 for RIF and INH, respectively. Conclusion: The RTM appears to be a reliable method for the rapid and simultaneous detection of MDR-TB and drug susceptibility testing (DST) of M. tuberculosis. It is simple, inexpensive, and with no biohazard risk involved.http://www.jgid.org/article.asp?issn=0974-777X;year=2014;volume=6;issue=4;spage=151;epage=156;aulast=PatilDrug susceptibilityMycobacterium tuberculosisRTM |
| spellingShingle | Santosh S Patil Shivajirao T Mohite Sunanda A Kulkarni Usha S Udgaonkar Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis Journal of Global Infectious Diseases Drug susceptibility Mycobacterium tuberculosis RTM |
| title | Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis |
| title_full | Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis |
| title_fullStr | Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis |
| title_full_unstemmed | Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis |
| title_short | Resazurin tube method: Rapid, simple, and inexpensive method for detection of drug resistance in the clinical isolates of Mycobacterium Tuberculosis |
| title_sort | resazurin tube method rapid simple and inexpensive method for detection of drug resistance in the clinical isolates of mycobacterium tuberculosis |
| topic | Drug susceptibility Mycobacterium tuberculosis RTM |
| url | http://www.jgid.org/article.asp?issn=0974-777X;year=2014;volume=6;issue=4;spage=151;epage=156;aulast=Patil |
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