Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’)
Volatiles are essential substances that determine distinct fruit flavors and user preferences. However, the metabolic dynamic and molecular modulation models that regulate the overall flavor generation during fruit growth and ripening are still largely unclear for most fruit species. To comprehensiv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/4/483 |
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| author | Huiyun Li Jine Quan Sohel Rana Shunyang Yao Yanmei Wang Zhi Li Qifei Cai Chaowang Ma Xiaodong Geng Zhen Liu |
| author_facet | Huiyun Li Jine Quan Sohel Rana Shunyang Yao Yanmei Wang Zhi Li Qifei Cai Chaowang Ma Xiaodong Geng Zhen Liu |
| author_sort | Huiyun Li |
| collection | DOAJ |
| description | Volatiles are essential substances that determine distinct fruit flavors and user preferences. However, the metabolic dynamic and molecular modulation models that regulate the overall flavor generation during fruit growth and ripening are still largely unclear for most fruit species. To comprehensively analyze the molecular mechanism and regulation mechanism of aroma accumulation and aroma component formation in <i>Pyrus</i> spp. ‘Panguxiang’ (‘Panguxiang’pear), this study compared pear phenotype, sugars, organic acid content, and the expression of related genes and metabolites amid pear growth and development in <i>Pyrus</i> spp. ‘Panguxiang’. A total of 417 VOCs (4 amines, 19 aromatics, 29 aldehydes, 31 alcohols, 38 ketones, 64 heterocyclic compounds, 89 terpenoids, 94 esters, and 49 others) were found. The potential gene expression patterns were explored by combining transcriptomics and metabolomics, and VOC-associated metabolism and transcriptome data from all samples were integrated during the growth and development period. On this basis, we constructed a colorful model depicting changes in the VOCs and genes throughout pear growth and development. Our findings reveal that terpenoid biosynthesis pathways are the main aroma production pathways during pear growth and development. In addition to providing novel insights into the metabolic control of fruit flavor during growth and development, this study also provides a new theoretical basis for studying aroma metabolites in pears. |
| first_indexed | 2024-03-11T04:58:28Z |
| format | Article |
| id | doaj.art-d272079d12124337acbe7d7d9c0f0ce2 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-11T04:58:28Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-d272079d12124337acbe7d7d9c0f0ce22023-11-17T19:29:24ZengMDPI AGHorticulturae2311-75242023-04-019448310.3390/horticulturae9040483Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’)Huiyun Li0Jine Quan1Sohel Rana2Shunyang Yao3Yanmei Wang4Zhi Li5Qifei Cai6Chaowang Ma7Xiaodong Geng8Zhen Liu9College of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaZhengzhou Zhengshi Chemical Co., Ltd., Zhengzhou 450002, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaVolatiles are essential substances that determine distinct fruit flavors and user preferences. However, the metabolic dynamic and molecular modulation models that regulate the overall flavor generation during fruit growth and ripening are still largely unclear for most fruit species. To comprehensively analyze the molecular mechanism and regulation mechanism of aroma accumulation and aroma component formation in <i>Pyrus</i> spp. ‘Panguxiang’ (‘Panguxiang’pear), this study compared pear phenotype, sugars, organic acid content, and the expression of related genes and metabolites amid pear growth and development in <i>Pyrus</i> spp. ‘Panguxiang’. A total of 417 VOCs (4 amines, 19 aromatics, 29 aldehydes, 31 alcohols, 38 ketones, 64 heterocyclic compounds, 89 terpenoids, 94 esters, and 49 others) were found. The potential gene expression patterns were explored by combining transcriptomics and metabolomics, and VOC-associated metabolism and transcriptome data from all samples were integrated during the growth and development period. On this basis, we constructed a colorful model depicting changes in the VOCs and genes throughout pear growth and development. Our findings reveal that terpenoid biosynthesis pathways are the main aroma production pathways during pear growth and development. In addition to providing novel insights into the metabolic control of fruit flavor during growth and development, this study also provides a new theoretical basis for studying aroma metabolites in pears.https://www.mdpi.com/2311-7524/9/4/483pearsvolatile aroma formationterpenoidsgene expressionmetabolic profiling |
| spellingShingle | Huiyun Li Jine Quan Sohel Rana Shunyang Yao Yanmei Wang Zhi Li Qifei Cai Chaowang Ma Xiaodong Geng Zhen Liu Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) Horticulturae pears volatile aroma formation terpenoids gene expression metabolic profiling |
| title | Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) |
| title_full | Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) |
| title_fullStr | Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) |
| title_full_unstemmed | Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) |
| title_short | Comprehensive Metabolomic and Transcriptomic Analysis of the Regulatory Network of Volatile Terpenoid Formation during the Growth and Development of Pears (<i>Pyrus</i> spp. ‘Panguxiang’) |
| title_sort | comprehensive metabolomic and transcriptomic analysis of the regulatory network of volatile terpenoid formation during the growth and development of pears i pyrus i spp panguxiang |
| topic | pears volatile aroma formation terpenoids gene expression metabolic profiling |
| url | https://www.mdpi.com/2311-7524/9/4/483 |
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