Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms
Agata Lange,1 Arkadiusz Matuszewski,2 Marta Kutwin,1 Agnieszka Ostrowska,1 Sławomir Jaworski1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Animal Environment Biology, Institute of Animal Sciences, Warsaw Univers...
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Dove Medical Press
2024-04-01
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| Series: | Nanotechnology, Science and Applications |
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| Online Access: | https://www.dovepress.com/farnesol-and-selected-nanoparticles-silver-gold-copper-and-zinc-oxide--peer-reviewed-fulltext-article-NSA |
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| author | Lange A Matuszewski A Kutwin M Ostrowska A Jaworski S |
| author_facet | Lange A Matuszewski A Kutwin M Ostrowska A Jaworski S |
| author_sort | Lange A |
| collection | DOAJ |
| description | Agata Lange,1 Arkadiusz Matuszewski,2 Marta Kutwin,1 Agnieszka Ostrowska,1 Sławomir Jaworski1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Animal Environment Biology, Institute of Animal Sciences, Warsaw University of Life Sciences, Warsaw, PolandCorrespondence: Sławomir Jaworski, Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8, Warsaw, 02-786, Poland, Tel +48 225936675, Email slawomir_jaworski@sggw.edu.plPurpose: Biofilms, which are created by most microorganisms, are known for their widely developed drug resistance, even more than planktonic forms of microorganisms. The aim of the study was to assess the effectiveness of agents composed of farnesol and nanoparticles (silver, gold, copper, and zinc oxide) in the degradation of biofilms produced by pathogenic microorganisms.Methods: Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans were used to create the biofilm structure. Colloidal suspensions of silver, gold, copper, and zinc oxide (Ag, Au, Cu, ZnO) with the addition of farnesol (F) were used as the treatment factor. The size distribution of those composites was analyzed, their zeta potential was measured, and their structure was visualized by transmission electron microscopy. The viability of the microorganism strains was assessed by an XTT assay, the ability to form biofilms was analyzed by confocal microscopy, and the changes in biofilm structure were evaluated by scanning electron microscopy. The general toxicity toward the HFFF2 cell line was determined by a neutral red assay and a human inflammation antibody array.Results: The link between the two components (farnesol and nanoparticles) caused mutual stability of both components. Planktonic forms of the microorganisms were the most sensitive when exposed to AgF and CuF; however, the biofilm structure of all microorganism strains was the most disrupted (both inhibition of formation and changes within the structure) after AgF treatment. Composites were not toxic toward the HFFF2 cell line, although the expression of several cytokines was higher than in the not-treated group.Conclusion: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.Keywords: farnesol, nanocomposites, nanoparticles, biofilm structure, toxicity |
| first_indexed | 2024-04-24T08:32:45Z |
| format | Article |
| id | doaj.art-d27247261a5b437abeb50d5f1f4606de |
| institution | Directory Open Access Journal |
| issn | 1177-8903 |
| language | English |
| last_indexed | 2024-04-24T08:32:45Z |
| publishDate | 2024-04-01 |
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| series | Nanotechnology, Science and Applications |
| spelling | doaj.art-d27247261a5b437abeb50d5f1f4606de2024-04-16T18:59:54ZengDove Medical PressNanotechnology, Science and Applications1177-89032024-04-01Volume 1710712591997Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic MicroorganismsLange AMatuszewski AKutwin MOstrowska AJaworski SAgata Lange,1 Arkadiusz Matuszewski,2 Marta Kutwin,1 Agnieszka Ostrowska,1 Sławomir Jaworski1 1Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Warsaw, Poland; 2Department of Animal Environment Biology, Institute of Animal Sciences, Warsaw University of Life Sciences, Warsaw, PolandCorrespondence: Sławomir Jaworski, Department of Nanobiotechnology, Institute of Biology, Warsaw University of Life Sciences, Ciszewskiego 8, Warsaw, 02-786, Poland, Tel +48 225936675, Email slawomir_jaworski@sggw.edu.plPurpose: Biofilms, which are created by most microorganisms, are known for their widely developed drug resistance, even more than planktonic forms of microorganisms. The aim of the study was to assess the effectiveness of agents composed of farnesol and nanoparticles (silver, gold, copper, and zinc oxide) in the degradation of biofilms produced by pathogenic microorganisms.Methods: Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans were used to create the biofilm structure. Colloidal suspensions of silver, gold, copper, and zinc oxide (Ag, Au, Cu, ZnO) with the addition of farnesol (F) were used as the treatment factor. The size distribution of those composites was analyzed, their zeta potential was measured, and their structure was visualized by transmission electron microscopy. The viability of the microorganism strains was assessed by an XTT assay, the ability to form biofilms was analyzed by confocal microscopy, and the changes in biofilm structure were evaluated by scanning electron microscopy. The general toxicity toward the HFFF2 cell line was determined by a neutral red assay and a human inflammation antibody array.Results: The link between the two components (farnesol and nanoparticles) caused mutual stability of both components. Planktonic forms of the microorganisms were the most sensitive when exposed to AgF and CuF; however, the biofilm structure of all microorganism strains was the most disrupted (both inhibition of formation and changes within the structure) after AgF treatment. Composites were not toxic toward the HFFF2 cell line, although the expression of several cytokines was higher than in the not-treated group.Conclusion: The in vitro studies demonstrated antibiofilm properties of composites based on farnesol and nanoparticles. The greatest changes in biofilm structure were triggered by AgF, causing an alteration in the biofilm formation process as well as in the biofilm structure.Keywords: farnesol, nanocomposites, nanoparticles, biofilm structure, toxicityhttps://www.dovepress.com/farnesol-and-selected-nanoparticles-silver-gold-copper-and-zinc-oxide--peer-reviewed-fulltext-article-NSAfarnesolnanocompositesnanoparticlesbiofilm structuretoxicity |
| spellingShingle | Lange A Matuszewski A Kutwin M Ostrowska A Jaworski S Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms Nanotechnology, Science and Applications farnesol nanocomposites nanoparticles biofilm structure toxicity |
| title | Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms |
| title_full | Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms |
| title_fullStr | Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms |
| title_full_unstemmed | Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms |
| title_short | Farnesol and Selected Nanoparticles (Silver, Gold, Copper, and Zinc Oxide) as Effective Agents Against Biofilms Formed by Pathogenic Microorganisms |
| title_sort | farnesol and selected nanoparticles silver gold copper and zinc oxide as effective agents against biofilms formed by pathogenic microorganisms |
| topic | farnesol nanocomposites nanoparticles biofilm structure toxicity |
| url | https://www.dovepress.com/farnesol-and-selected-nanoparticles-silver-gold-copper-and-zinc-oxide--peer-reviewed-fulltext-article-NSA |
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