Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria
AbstractThis study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2268167 |
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| author | Rohazila Mohamad Hanafiah Siti Aisyah Abd Ghafar Vuanghao Lim Siti Nor Asma Musa Fahmi Yakop Arif Haikal Hairil Anuar |
| author_facet | Rohazila Mohamad Hanafiah Siti Aisyah Abd Ghafar Vuanghao Lim Siti Nor Asma Musa Fahmi Yakop Arif Haikal Hairil Anuar |
| author_sort | Rohazila Mohamad Hanafiah |
| collection | DOAJ |
| description | AbstractThis study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis of AgNPs. The synthesized AgNPs were characterized by using UV-Vis spectrophotometer, dynamic light scattering (DLS), field emission scanning electron microscope (FESEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR). FESEM images showed a rough surface with a spherical shape. The average size distribution of 75.25 nm with a polydispersity index (PDI) of 0.373. XRD analysis matched the face-centred cubic structure of silver. FTIR analysis revealed a shifted peak from 1404.99 to 1345.00 cm−1. MIC and MBC values of SC-AgNPs were 1.25 mg/mL and 2.5 mg/mL against E. coli, P. aeruginosa and S. mutans, respectively. Time-kill assay showed that SC-AgNPs significantly reduced bacterial growth as compared to non-treated bacteria. Morphologies of bacteria treated with SC-AgNPs were shrunk, lysed, irregular and smaller as compared to control. SC-AgNPs significantly disrupted the gene expression of eae A, gtf B and Pel A (p < 0.05). This study indicated that the synthesized SC-AgNPs were stable with enhanced antibacterial activities. |
| first_indexed | 2024-03-11T11:17:53Z |
| format | Article |
| id | doaj.art-d27437f6a5dd4c058e0bae27ba18acbf |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:17:53Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-d27437f6a5dd4c058e0bae27ba18acbf2023-11-10T17:19:32ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151154955910.1080/21691401.2023.2268167Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteriaRohazila Mohamad Hanafiah0Siti Aisyah Abd Ghafar1Vuanghao Lim2Siti Nor Asma Musa3Fahmi Yakop4Arif Haikal Hairil Anuar5Department of Basic Science, Faculty of Dentistry, Universiti Sains Islam Malaysia, Ampang Kuala Lumpur, MalaysiaDepartment of Basic Science, Faculty of Dentistry, Universiti Sains Islam Malaysia, Ampang Kuala Lumpur, MalaysiaIntegrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Kepala Batas, Penang, MalaysiaDepartment of Basic Science, Faculty of Dentistry, Universiti Sains Islam Malaysia, Ampang Kuala Lumpur, MalaysiaDepartment of Basic Science, Faculty of Dentistry, Universiti Sains Islam Malaysia, Ampang Kuala Lumpur, MalaysiaDepartment of Basic Science, Faculty of Dentistry, Universiti Sains Islam Malaysia, Ampang Kuala Lumpur, MalaysiaAbstractThis study aims to characterize and determine the antibacterial activities of synthesized Strobilanthes crispus-mediated AgNPs (SC-AgNPs) against Streptococcus mutans, Escherichia coli and Pseudomonas aeruginosa. S. crispus water extract acts as a reducing and capping agent in the synthesis of AgNPs. The synthesized AgNPs were characterized by using UV-Vis spectrophotometer, dynamic light scattering (DLS), field emission scanning electron microscope (FESEM), X-ray diffractometer (XRD) and Fourier transform infra-red (FTIR). FESEM images showed a rough surface with a spherical shape. The average size distribution of 75.25 nm with a polydispersity index (PDI) of 0.373. XRD analysis matched the face-centred cubic structure of silver. FTIR analysis revealed a shifted peak from 1404.99 to 1345.00 cm−1. MIC and MBC values of SC-AgNPs were 1.25 mg/mL and 2.5 mg/mL against E. coli, P. aeruginosa and S. mutans, respectively. Time-kill assay showed that SC-AgNPs significantly reduced bacterial growth as compared to non-treated bacteria. Morphologies of bacteria treated with SC-AgNPs were shrunk, lysed, irregular and smaller as compared to control. SC-AgNPs significantly disrupted the gene expression of eae A, gtf B and Pel A (p < 0.05). This study indicated that the synthesized SC-AgNPs were stable with enhanced antibacterial activities.https://www.tandfonline.com/doi/10.1080/21691401.2023.2268167Silver nanoparticlesStrobilanthes crispusantibacterialStreptococcus mutansEscherichia coliPseudomonas aeruginosa |
| spellingShingle | Rohazila Mohamad Hanafiah Siti Aisyah Abd Ghafar Vuanghao Lim Siti Nor Asma Musa Fahmi Yakop Arif Haikal Hairil Anuar Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria Artificial Cells, Nanomedicine, and Biotechnology Silver nanoparticles Strobilanthes crispus antibacterial Streptococcus mutans Escherichia coli Pseudomonas aeruginosa |
| title | Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria |
| title_full | Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria |
| title_fullStr | Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria |
| title_full_unstemmed | Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria |
| title_short | Green synthesis, characterisation and antibacterial activities of Strobilanthes crispus-mediated silver nanoparticles (SC-AGNPS) against selected bacteria |
| title_sort | green synthesis characterisation and antibacterial activities of strobilanthes crispus mediated silver nanoparticles sc agnps against selected bacteria |
| topic | Silver nanoparticles Strobilanthes crispus antibacterial Streptococcus mutans Escherichia coli Pseudomonas aeruginosa |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2268167 |
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