Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/49779 |
| _version_ | 1811252719273377792 |
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| author | Claudia Prahst Parham Ashrafzadeh Thomas Mead Ana Figueiredo Karen Chang Douglas Richardson Lakshmi Venkaraman Mark Richards Ana Martins Russo Kyle Harrington Marie Ouarné Andreia Pena Dong Feng Chen Lena Claesson-Welsh Kin-Sang Cho Claudio A Franco Katie Bentley |
| author_facet | Claudia Prahst Parham Ashrafzadeh Thomas Mead Ana Figueiredo Karen Chang Douglas Richardson Lakshmi Venkaraman Mark Richards Ana Martins Russo Kyle Harrington Marie Ouarné Andreia Pena Dong Feng Chen Lena Claesson-Welsh Kin-Sang Cho Claudio A Franco Katie Bentley |
| author_sort | Claudia Prahst |
| collection | DOAJ |
| description | As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated ‘knotted’ morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes. |
| first_indexed | 2024-04-12T16:38:55Z |
| format | Article |
| id | doaj.art-d277767636ea4911b66efae5c50328a8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:38:55Z |
| publishDate | 2020-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d277767636ea4911b66efae5c50328a82022-12-22T03:24:53ZengeLife Sciences Publications LtdeLife2050-084X2020-02-01910.7554/eLife.49779Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopyClaudia Prahst0Parham Ashrafzadeh1Thomas Mead2https://orcid.org/0000-0003-2728-670XAna Figueiredo3Karen Chang4Douglas Richardson5Lakshmi Venkaraman6Mark Richards7Ana Martins Russo8Kyle Harrington9Marie Ouarné10Andreia Pena11Dong Feng Chen12https://orcid.org/0000-0001-6283-8843Lena Claesson-Welsh13https://orcid.org/0000-0003-4275-2000Kin-Sang Cho14https://orcid.org/0000-0003-4285-615XClaudio A Franco15https://orcid.org/0000-0002-2861-3883Katie Bentley16https://orcid.org/0000-0002-9391-659XCenter for Vascular Biology Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, United StatesThe Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SwedenThe Francis Crick Institute, London, United Kingdom; Department of Informatics, Faculty of Natural and Mathematical Sciences, Kings College London, London, United KingdomInstituto de Medicina Molecular, Lisbon, PortugalSchepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, United StatesHarvard Center for Biological Imaging, Department of Molecular and Cellular Biology, Harvard University, Cambridge, United StatesCenter for Vascular Biology Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, United States; The Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SwedenThe Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SwedenInstituto de Medicina Molecular, Lisbon, PortugalCenter for Vascular Biology Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, United StatesInstituto de Medicina Molecular, Lisbon, PortugalInstituto de Medicina Molecular, Lisbon, PortugalSchepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, United StatesThe Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, SwedenSchepens Eye Research Institute of Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, United States; Geriatric Research Education and Clinical Center, Office of Research and Development, Edith Nourse Rogers Memorial Veterans Hospital, Bedford, United StatesInstituto de Medicina Molecular, Lisbon, PortugalCenter for Vascular Biology Research and Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, United States; The Beijer Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden; The Francis Crick Institute, London, United Kingdom; Department of Informatics, Faculty of Natural and Mathematical Sciences, Kings College London, London, United Kingdom; Biomedical Engineering Department, Boston University, Boston, United StatesAs the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models. Here, we demonstrate that 1) rapid, quantitative 3D and 4D (time lapse) imaging of cellular and subcellular processes in the mouse eye is feasible, with and without tissue clearing, using light-sheet fluorescent microscopy (LSFM); 2) flat-mounting retinas for confocal microscopy significantly distorts tissue morphology, confirmed by quantitative correlative LSFM-Confocal imaging of vessels; 3) LSFM readily reveals new features of even well-studied eye disease mouse models, such as the oxygen-induced retinopathy (OIR) model, including a previously unappreciated ‘knotted’ morphology to pathological vascular tufts, abnormal cell motility and altered filopodia dynamics when live-imaged. We conclude that quantitative 3D/4D LSFM imaging and analysis has the potential to advance our understanding of the eye, in particular pathological, neurovascular, degenerative processes.https://elifesciences.org/articles/49779confocal microscopylightsheet microscopymouse retinaangiogenesisretinopathy of prematurityneurovascular |
| spellingShingle | Claudia Prahst Parham Ashrafzadeh Thomas Mead Ana Figueiredo Karen Chang Douglas Richardson Lakshmi Venkaraman Mark Richards Ana Martins Russo Kyle Harrington Marie Ouarné Andreia Pena Dong Feng Chen Lena Claesson-Welsh Kin-Sang Cho Claudio A Franco Katie Bentley Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy eLife confocal microscopy lightsheet microscopy mouse retina angiogenesis retinopathy of prematurity neurovascular |
| title | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| title_full | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| title_fullStr | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| title_full_unstemmed | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| title_short | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| title_sort | mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy |
| topic | confocal microscopy lightsheet microscopy mouse retina angiogenesis retinopathy of prematurity neurovascular |
| url | https://elifesciences.org/articles/49779 |
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