Evaluation of Vinegar as a Disinfectant for Extracted Human Teeth - An in-Vitro Study
Introduction: In dentistry, extracted human teeth are routinely used to learn technical and preclinical skills. Since human teeth harbour many pathogens these should be disinfected before use to minimize the risk of infections. Some commonly used disinfectants in laboratories are 10% formalin, 3...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2016-07-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/8167/19025_CE(EK)_F(AK)_PF1(NEAK)_PFA(AK)_PF2(PAG).pdf |
Summary: | Introduction: In dentistry, extracted human teeth are routinely
used to learn technical and preclinical skills. Since human teeth
harbour many pathogens these should be disinfected before
use to minimize the risk of infections. Some commonly used
disinfectants in laboratories are 10% formalin, 3% hydrogen
peroxide (H2
O2
), 5.25% Sodium Hypochlorite (Naocl), 70%
alcohol and normal saline which have their own disadvantages
like carcinogenicity, toxicity, cost effectiveness etc. Many
studies have been conducted using these solutions but there
is no evidence to suggest a suitable alternative for disinfecting
extracted teeth. Vinegar is a sour liquid comprised mainly of
acetic acid. It is cheap and commercially available shown
to be effective in the prevention and control of microbial
contamination.
Aim: The present study was conducted for evaluation of vinegar
as a disinfectant for extracted teeth.
Materials and Methods: In this study a total of 40 (n=40)
extracted non carious teeth were taken which were disinfected
with various physical methods such as sterilization, autoclaving
and chemical methods by using Vinegar, 70% Alcohol, 10%
Formalin, 3% Hydrogen peroxide and 5.25% NaOCL. Later,
teeth from each group were placed individually in separate test
tubes containing 10ml of brain heart infusion broth at 37°C for
48 hrs to observe the evidence of growth of microorganisms.
Results: Results were analyzed using Kruskal-Wallis test.
Vinegar, 10% Formalin and 3% Hydrogen peroxide were
effective. The results were statistically significant with KruskalWallis test value 28.053 and p-value was <0.001.
Conclusion: Vinegar can be used as an effective disinfectant
for extracted human teeth. |
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ISSN: | 2249-782X 0973-709X |