Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury
Background. Cold injury is one of the most significant problems for the northern regions of the Russian Federation. Frostbite is defined as a complex of changes caused by the action of low temperatures, which lead to morphological changes in damaged tissue structures. As a result, the skin with unde...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Scientific Сentre for Family Health and Human Reproduction Problems
2023-10-01
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| Series: | Acta Biomedica Scientifica |
| Subjects: | |
| Online Access: | https://www.actabiomedica.ru/jour/article/view/4346 |
| _version_ | 1797237162069983232 |
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| author | O. S. Gruzdeva R. V. Gruzdev I. A. Rylskiy K. G. Shapovalov |
| author_facet | O. S. Gruzdeva R. V. Gruzdev I. A. Rylskiy K. G. Shapovalov |
| author_sort | O. S. Gruzdeva |
| collection | DOAJ |
| description | Background. Cold injury is one of the most significant problems for the northern regions of the Russian Federation. Frostbite is defined as a complex of changes caused by the action of low temperatures, which lead to morphological changes in damaged tissue structures. As a result, the skin with underlying tissues and the intercellular matrix, the components of which are collagen fibers, are damaged, which eventually leads to remodeling and a protracted course of the wound process. Morphometric studies in combination with quantitative analysis of microphotographs (histological specimens) using GIS technologies make it possible to distinguish altered collagen fibers under the influence of low temperatures from relatively healthy tissues. The aim of the study. To assess the possibility of using computer analysis of microphotographs in a complex of morphometric studies of collagen fibers in local cold injury. Materials and methods. The study included 84 patients with III and IV degree frostbite of the lower extremities. Morphological study of tissues and microphotography were performed using Leica DM2500 microscope (Leica, Germany). The thickness of collagen fibers was measured based on visual measurements of characteristic areas of the microphotograph. Computer analysis of tissue microphotographs of the zone of cryoinjury was performed using the ArcINFO software (Esri, USA). Statistical processing of the study results was carried out using the SPSS Statistics software package (IBM Corp., USA). Graphs and diagrams were constructed using MS Office Excel (Microsoft Corp., USA). Results. With frostbite, severe tissue damage occurs, accompanied by the destructive processes of the extracellular matrix components. Low temperatures contribute to changes in the width and orientation of collagen fibers in the damaged area. In this regard, a change in the texture of the histological specimen image leads to achange in the numerical characteristics of the standard deviation of the curvature coefficient in the studied area of the microphotograph. Thus, in the late reactive period, the described complex of morphometric studies makes it possible to classify particular microphotograph as having pathological signs or as a sample of healthy tissue. |
| first_indexed | 2024-03-08T12:15:20Z |
| format | Article |
| id | doaj.art-d27a5f2b5c9b4f26b320bb29e8028969 |
| institution | Directory Open Access Journal |
| issn | 2541-9420 2587-9596 |
| language | Russian |
| last_indexed | 2024-04-24T17:15:21Z |
| publishDate | 2023-10-01 |
| publisher | Scientific Сentre for Family Health and Human Reproduction Problems |
| record_format | Article |
| series | Acta Biomedica Scientifica |
| spelling | doaj.art-d27a5f2b5c9b4f26b320bb29e80289692024-03-28T07:49:29ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962023-10-018414715410.29413/ABS.2023-8.4.162614Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injuryO. S. Gruzdeva0R. V. Gruzdev1I. A. Rylskiy2K. G. Shapovalov3Chita State Medical AcademyNN Technical Services LLCLomonosov Moscow State UniversityChita State Medical AcademyBackground. Cold injury is one of the most significant problems for the northern regions of the Russian Federation. Frostbite is defined as a complex of changes caused by the action of low temperatures, which lead to morphological changes in damaged tissue structures. As a result, the skin with underlying tissues and the intercellular matrix, the components of which are collagen fibers, are damaged, which eventually leads to remodeling and a protracted course of the wound process. Morphometric studies in combination with quantitative analysis of microphotographs (histological specimens) using GIS technologies make it possible to distinguish altered collagen fibers under the influence of low temperatures from relatively healthy tissues. The aim of the study. To assess the possibility of using computer analysis of microphotographs in a complex of morphometric studies of collagen fibers in local cold injury. Materials and methods. The study included 84 patients with III and IV degree frostbite of the lower extremities. Morphological study of tissues and microphotography were performed using Leica DM2500 microscope (Leica, Germany). The thickness of collagen fibers was measured based on visual measurements of characteristic areas of the microphotograph. Computer analysis of tissue microphotographs of the zone of cryoinjury was performed using the ArcINFO software (Esri, USA). Statistical processing of the study results was carried out using the SPSS Statistics software package (IBM Corp., USA). Graphs and diagrams were constructed using MS Office Excel (Microsoft Corp., USA). Results. With frostbite, severe tissue damage occurs, accompanied by the destructive processes of the extracellular matrix components. Low temperatures contribute to changes in the width and orientation of collagen fibers in the damaged area. In this regard, a change in the texture of the histological specimen image leads to achange in the numerical characteristics of the standard deviation of the curvature coefficient in the studied area of the microphotograph. Thus, in the late reactive period, the described complex of morphometric studies makes it possible to classify particular microphotograph as having pathological signs or as a sample of healthy tissue.https://www.actabiomedica.ru/jour/article/view/4346morphometrycollagen fiberscryoinjuryfrostbitestandard deviationcurvature coefficientraster image |
| spellingShingle | O. S. Gruzdeva R. V. Gruzdev I. A. Rylskiy K. G. Shapovalov Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury Acta Biomedica Scientifica morphometry collagen fibers cryoinjury frostbite standard deviation curvature coefficient raster image |
| title | Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| title_full | Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| title_fullStr | Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| title_full_unstemmed | Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| title_short | Automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| title_sort | automated morphometric studies of collagen fibers as an auxiliary method for diagnosing cold injury |
| topic | morphometry collagen fibers cryoinjury frostbite standard deviation curvature coefficient raster image |
| url | https://www.actabiomedica.ru/jour/article/view/4346 |
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