A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study
Objectives The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929)....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Academy of Conservative Dentistry
2013-11-01
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| Series: | Restorative Dentistry & Endodontics |
| Online Access: | https://doi.org/10.5395/rde.2013.38.4.204 |
| _version_ | 1818454091364302848 |
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| author | Gautam Pyarelal Badole Manjusha Madhukar Warhadpande Ganesh Kothiramji Meshram Rakesh Namdeoraoji Bahadure Shubha Gopal Tawani Gopal Tawani Shital Gautam Badole |
| author_facet | Gautam Pyarelal Badole Manjusha Madhukar Warhadpande Ganesh Kothiramji Meshram Rakesh Namdeoraoji Bahadure Shubha Gopal Tawani Gopal Tawani Shital Gautam Badole |
| author_sort | Gautam Pyarelal Badole |
| collection | DOAJ |
| description | Objectives
The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929).
Materials and Methods
Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis.
Results
Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity.
Conclusions
Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time. |
| first_indexed | 2024-12-14T21:49:22Z |
| format | Article |
| id | doaj.art-d27c517707cf43bb9c1b5162b98a8f48 |
| institution | Directory Open Access Journal |
| issn | 2234-7658 2234-7666 |
| language | English |
| last_indexed | 2024-12-14T21:49:22Z |
| publishDate | 2013-11-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj.art-d27c517707cf43bb9c1b5162b98a8f482022-12-21T22:46:16ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662013-11-0138420420910.5395/rde.2013.38.4.204A comparative evaluation of cytotoxicity of root canal sealers: an in vitro studyGautam Pyarelal Badole0Manjusha Madhukar Warhadpande1Ganesh Kothiramji Meshram2Rakesh Namdeoraoji Bahadure3Shubha Gopal Tawani4Gopal Tawani5Shital Gautam Badole6Department of Conservative Dentistry & Endodontics VSPM's Dental College & Research Center, Nagpur, India.Department of Conservative Dentistry & Endodontics Government Dental College & Hospital, Nagpur, India.Department of Conservative Dentistry & Endodontics, Peoples Dental College, Bhopal, India.Department of Pedodontics, Sharad Pawar Dental College, Sawangi, Wardha, India.Department of Conservative Dentistry & Endodontics Government Dental College & Hospital, Nagpur, India.Department of Conservative Dentistry & Endodontics Government Dental College & Hospital, Nagpur, India.Department of Conservative Dentistry & Endodontics VSPM's Dental College & Research Center, Nagpur, India.Objectives The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929). Materials and Methods Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis. Results Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity. Conclusions Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.https://doi.org/10.5395/rde.2013.38.4.204 |
| spellingShingle | Gautam Pyarelal Badole Manjusha Madhukar Warhadpande Ganesh Kothiramji Meshram Rakesh Namdeoraoji Bahadure Shubha Gopal Tawani Gopal Tawani Shital Gautam Badole A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study Restorative Dentistry & Endodontics |
| title | A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study |
| title_full | A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study |
| title_fullStr | A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study |
| title_full_unstemmed | A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study |
| title_short | A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study |
| title_sort | comparative evaluation of cytotoxicity of root canal sealers an in vitro study |
| url | https://doi.org/10.5395/rde.2013.38.4.204 |
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