mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients
Abstract Background The etiopathogenesis of coronavirus disease 2019 (COVID-19) stem partially from the abnormal activation of the innate and adaptive immune systems. Here in the current investigation, the mRNA expression levels of toll-like receptors (TLRs) were evaluated in the nasopharyngeal epit...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-022-07437-9 |
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| author | Zahra Bagheri-Hosseinabadi Ebrahim Rezazadeh Zarandi Mohammad Mirabzadeh Ali Amiri Mitra Abbasifard |
| author_facet | Zahra Bagheri-Hosseinabadi Ebrahim Rezazadeh Zarandi Mohammad Mirabzadeh Ali Amiri Mitra Abbasifard |
| author_sort | Zahra Bagheri-Hosseinabadi |
| collection | DOAJ |
| description | Abstract Background The etiopathogenesis of coronavirus disease 2019 (COVID-19) stem partially from the abnormal activation of the innate and adaptive immune systems. Here in the current investigation, the mRNA expression levels of toll-like receptors (TLRs) were evaluated in the nasopharyngeal epithelial cells from COVID-19 patients. Methods Epithelial cells were obtained using nasopharyngeal swab samples from 90 COVID-19 patients and 50 controls. COVID-19 cases were classified into those without symptoms, with symptoms but not hospitalized, and with symptoms and hospitalized. To determine the mRNA expression levels of TLRs, first RNA was extracted and cDNA was synthesized, and finally Real-time PCR was exerted. Results It was seen that the transcript levels of TLR3, TLR7, TLR8, and TLR9 were overexpressed in the COVID-19 patients with clinical symptoms needing hospitalization as well as in those with clinical symptoms without needing for hospitalization compared to controls. Upregulation of TLRs was associated with clinical presentations of the patients. Conclusions Modulation of TLR3, TLR7, TLR8, TLR9 in the epithelial cells of COVID-19 cases may estimate the disease severity and requirement for hospitalization. |
| first_indexed | 2024-12-12T03:05:21Z |
| format | Article |
| id | doaj.art-d27febaeadfe4c2aadfd7feff7623575 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-12T03:05:21Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-d27febaeadfe4c2aadfd7feff76235752022-12-22T00:40:32ZengBMCBMC Infectious Diseases1471-23342022-05-0122111010.1186/s12879-022-07437-9mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patientsZahra Bagheri-Hosseinabadi0Ebrahim Rezazadeh Zarandi1Mohammad Mirabzadeh2Ali Amiri3Mitra Abbasifard4Pistachio Safety Research Center, Rafsanjan University of Medical SciencesImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical SciencesStudent Research Committee, Rafsanjan University of Medical SciencesDepartment of Orthodontics, College of Stomatology, The First Affiliated Stomatological Hospital, Xi’an Jiaotong UniversityImmunology of Infectious Diseases Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical SciencesAbstract Background The etiopathogenesis of coronavirus disease 2019 (COVID-19) stem partially from the abnormal activation of the innate and adaptive immune systems. Here in the current investigation, the mRNA expression levels of toll-like receptors (TLRs) were evaluated in the nasopharyngeal epithelial cells from COVID-19 patients. Methods Epithelial cells were obtained using nasopharyngeal swab samples from 90 COVID-19 patients and 50 controls. COVID-19 cases were classified into those without symptoms, with symptoms but not hospitalized, and with symptoms and hospitalized. To determine the mRNA expression levels of TLRs, first RNA was extracted and cDNA was synthesized, and finally Real-time PCR was exerted. Results It was seen that the transcript levels of TLR3, TLR7, TLR8, and TLR9 were overexpressed in the COVID-19 patients with clinical symptoms needing hospitalization as well as in those with clinical symptoms without needing for hospitalization compared to controls. Upregulation of TLRs was associated with clinical presentations of the patients. Conclusions Modulation of TLR3, TLR7, TLR8, TLR9 in the epithelial cells of COVID-19 cases may estimate the disease severity and requirement for hospitalization.https://doi.org/10.1186/s12879-022-07437-9Severe acute respiratory syndrome coronavirus 2Coronavirus disease 2019InflammationToll-like receptorEpithelial cell |
| spellingShingle | Zahra Bagheri-Hosseinabadi Ebrahim Rezazadeh Zarandi Mohammad Mirabzadeh Ali Amiri Mitra Abbasifard mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients BMC Infectious Diseases Severe acute respiratory syndrome coronavirus 2 Coronavirus disease 2019 Inflammation Toll-like receptor Epithelial cell |
| title | mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| title_full | mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| title_fullStr | mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| title_full_unstemmed | mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| title_short | mRNA expression of toll-like receptors 3, 7, 8, and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| title_sort | mrna expression of toll like receptors 3 7 8 and 9 in the nasopharyngeal epithelial cells of coronavirus disease 2019 patients |
| topic | Severe acute respiratory syndrome coronavirus 2 Coronavirus disease 2019 Inflammation Toll-like receptor Epithelial cell |
| url | https://doi.org/10.1186/s12879-022-07437-9 |
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