Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors
Affymetrix microarray data and Northern blot assays demonstrated that phospholipid transfer protein (PL222222222216) was induced 6-fold when either murine or human macrophages were incubated in the presence of ligands for the liver X receptor (LXR) and the retinoid X receptor. Two functional LXR res...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2002-12-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520327292 |
| _version_ | 1830190385069031424 |
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| author | Puiying A. Mak Heidi R. Kast-Woelbern Andrew M. Anisfeld Peter A. Edwards |
| author_facet | Puiying A. Mak Heidi R. Kast-Woelbern Andrew M. Anisfeld Peter A. Edwards |
| author_sort | Puiying A. Mak |
| collection | DOAJ |
| description | Affymetrix microarray data and Northern blot assays demonstrated that phospholipid transfer protein (PL222222222216) was induced 6-fold when either murine or human macrophages were incubated in the presence of ligands for the liver X receptor (LXR) and the retinoid X receptor. Two functional LXR response elements (LXREs) were identified and characterized in the proximal promoter of the human PLTP gene. One LXRE corresponds to a traditional direct repeat separated by 4 bp. However, the second LXRE is novel in that it corresponds to an inverted repeat separated by 1 bp, and is identical to the farnesoid X receptor response element. These studies demonstrate that PLTP is a direct target for activated LXR and farnesoid X receptor (FXR). In addition, apolipoprotein E (apoE), a known LXR target gene in macrophages, was shown to be activated in liver cells by FXR ligands.Taken together, the current data suggest that a small number of genes that currently include PLTP, apoE, and apoC-II, are induced in macrophages by activated LXR and in liver by activated FXR. |
| first_indexed | 2024-12-17T23:20:57Z |
| format | Article |
| id | doaj.art-d284822ada134e6cab5da80089928525 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T23:20:57Z |
| publishDate | 2002-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-d284822ada134e6cab5da800899285252022-12-21T21:28:54ZengElsevierJournal of Lipid Research0022-22752002-12-01431220372041Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptorsPuiying A. Mak0Heidi R. Kast-Woelbern1Andrew M. Anisfeld2Peter A. Edwards3Departments of Biological Chemistry and Medicine, University of California, Los Angeles, CA 90095; Molecular Biology Institute, University of California, Los Angeles, CA 90095Departments of Biological Chemistry and Medicine, University of California, Los Angeles, CA 90095; Molecular Biology Institute, University of California, Los Angeles, CA 90095Departments of Biological Chemistry and Medicine, University of California, Los Angeles, CA 90095; Molecular Biology Institute, University of California, Los Angeles, CA 90095Departments of Biological Chemistry and Medicine, University of California, Los Angeles, CA 90095; Molecular Biology Institute, University of California, Los Angeles, CA 90095Affymetrix microarray data and Northern blot assays demonstrated that phospholipid transfer protein (PL222222222216) was induced 6-fold when either murine or human macrophages were incubated in the presence of ligands for the liver X receptor (LXR) and the retinoid X receptor. Two functional LXR response elements (LXREs) were identified and characterized in the proximal promoter of the human PLTP gene. One LXRE corresponds to a traditional direct repeat separated by 4 bp. However, the second LXRE is novel in that it corresponds to an inverted repeat separated by 1 bp, and is identical to the farnesoid X receptor response element. These studies demonstrate that PLTP is a direct target for activated LXR and farnesoid X receptor (FXR). In addition, apolipoprotein E (apoE), a known LXR target gene in macrophages, was shown to be activated in liver cells by FXR ligands.Taken together, the current data suggest that a small number of genes that currently include PLTP, apoE, and apoC-II, are induced in macrophages by activated LXR and in liver by activated FXR.http://www.sciencedirect.com/science/article/pii/S0022227520327292macrophagesfoam cellshepatocytesphospholipid transfer proteinliver X receptorretinoid X receptor |
| spellingShingle | Puiying A. Mak Heidi R. Kast-Woelbern Andrew M. Anisfeld Peter A. Edwards Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors Journal of Lipid Research macrophages foam cells hepatocytes phospholipid transfer protein liver X receptor retinoid X receptor |
| title | Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors |
| title_full | Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors |
| title_fullStr | Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors |
| title_full_unstemmed | Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors |
| title_short | Identification of PLTP as an LXR target gene and apoE as an FXR target gene reveals overlapping targets for the two nuclear receptors |
| title_sort | identification of pltp as an lxr target gene and apoe as an fxr target gene reveals overlapping targets for the two nuclear receptors |
| topic | macrophages foam cells hepatocytes phospholipid transfer protein liver X receptor retinoid X receptor |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520327292 |
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