miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6
Blood–brain barrier participates in the pathological process of ischemic stroke. MicroRNA-29c-5p was highly expressed in clinical samples from patients with ischemic stroke. In this study, oxygen-glucose deprivation (OGD) treatment of astrocytes enhanced the permeability of brain microvascular endot...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2022-02-01
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| Series: | Open Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/med-2022-0438 |
| _version_ | 1797998397934796800 |
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| author | Dai Qijun Sun Jian Dai Tianyi Xu Qin Ding Yueqin |
| author_facet | Dai Qijun Sun Jian Dai Tianyi Xu Qin Ding Yueqin |
| author_sort | Dai Qijun |
| collection | DOAJ |
| description | Blood–brain barrier participates in the pathological process of ischemic stroke. MicroRNA-29c-5p was highly expressed in clinical samples from patients with ischemic stroke. In this study, oxygen-glucose deprivation (OGD) treatment of astrocytes enhanced the permeability of brain microvascular endothelial cells (BMECs), and the miR-29c-5p expression was elevated in clinical samples from patients with ischemic stroke. For the function of miR-29c-5p in ischemic stroke, the miR-29c-5p knockdown decreased the permeability and the tight junction protein (TJP) destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes. Mechanistically, miR-29c-5p interacted with lipoprotein receptor-related protein 6 (LRP6) and negatively regulated the LRP6 expression in astrocytes. Moreover, the rescue assays indicated that the interference with miR-29c-5p ameliorated the TJP destruction of BMECs and inflammation caused by OGD-treated astrocytes by increasing the LRP6 expression. Together, miR-29c-5p knockdown decreased the high permeability and the TJP destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes by elevating LRP6 expression. |
| first_indexed | 2024-04-11T10:48:09Z |
| format | Article |
| id | doaj.art-d285a34ff86647658d547cd7412e9d3a |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-11T10:48:09Z |
| publishDate | 2022-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Medicine |
| spelling | doaj.art-d285a34ff86647658d547cd7412e9d3a2022-12-22T04:29:00ZengDe GruyterOpen Medicine2391-54632022-02-0117135336410.1515/med-2022-0438miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6Dai Qijun0Sun Jian1Dai Tianyi2Xu Qin3Ding Yueqin4Department of Neurology, Haian Hospital of Traditional Chinese Medicine, Haian, 226600, ChinaDepartment of Endocrinology, Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang, 214500, ChinaCollege of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Class 1802, Nanjing, 210023, ChinaDepartment of Neurology, Haian Hospital of Traditional Chinese Medicine, Haian, 226600, ChinaDepartment of Nursing, Haian Hospital of Traditional Chinese Medicine, Haian, 226600, ChinaBlood–brain barrier participates in the pathological process of ischemic stroke. MicroRNA-29c-5p was highly expressed in clinical samples from patients with ischemic stroke. In this study, oxygen-glucose deprivation (OGD) treatment of astrocytes enhanced the permeability of brain microvascular endothelial cells (BMECs), and the miR-29c-5p expression was elevated in clinical samples from patients with ischemic stroke. For the function of miR-29c-5p in ischemic stroke, the miR-29c-5p knockdown decreased the permeability and the tight junction protein (TJP) destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes. Mechanistically, miR-29c-5p interacted with lipoprotein receptor-related protein 6 (LRP6) and negatively regulated the LRP6 expression in astrocytes. Moreover, the rescue assays indicated that the interference with miR-29c-5p ameliorated the TJP destruction of BMECs and inflammation caused by OGD-treated astrocytes by increasing the LRP6 expression. Together, miR-29c-5p knockdown decreased the high permeability and the TJP destruction of BMECs and ameliorated the inflammation induced by OGD-treated astrocytes by elevating LRP6 expression.https://doi.org/10.1515/med-2022-0438ischemic strokeblood–brain barrierbrain microvascular endothelial cellsastrocytesmirna |
| spellingShingle | Dai Qijun Sun Jian Dai Tianyi Xu Qin Ding Yueqin miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 Open Medicine ischemic stroke blood–brain barrier brain microvascular endothelial cells astrocytes mirna |
| title | miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 |
| title_full | miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 |
| title_fullStr | miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 |
| title_full_unstemmed | miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 |
| title_short | miR-29c-5p knockdown reduces inflammation and blood–brain barrier disruption by upregulating LRP6 |
| title_sort | mir 29c 5p knockdown reduces inflammation and blood brain barrier disruption by upregulating lrp6 |
| topic | ischemic stroke blood–brain barrier brain microvascular endothelial cells astrocytes mirna |
| url | https://doi.org/10.1515/med-2022-0438 |
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