Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis
Abstract Conventional 3D cell culture methods require a comprehensive complement in labor‐intensive and time‐consuming processes along with in vivo circumstantial mimicking. Here, we describe a subaqueous free‐standing 3D cell culture (FS) device that can induce the omnidirectional environment and g...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10438 |
| _version_ | 1827990469500469248 |
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| author | Gwang‐Bum Im Yu‐Jin Kim Tae Il Lee Suk Ho Bhang |
| author_facet | Gwang‐Bum Im Yu‐Jin Kim Tae Il Lee Suk Ho Bhang |
| author_sort | Gwang‐Bum Im |
| collection | DOAJ |
| description | Abstract Conventional 3D cell culture methods require a comprehensive complement in labor‐intensive and time‐consuming processes along with in vivo circumstantial mimicking. Here, we describe a subaqueous free‐standing 3D cell culture (FS) device that can induce the omnidirectional environment and generate ultrafast human adipose‐derived stem cells (hADSCs) that efficiently aggregate with compaction using acoustic pressure. The cell culture conditions were optimized using the FS device and identified the underlying molecular mechanisms. Unique phenomena in cell aggregation have led to extraordinary cellular behavior that can upregulate cell compaction, mechanosensitive immune control, and therapeutic angiogenesis. Therefore, we designated the resulting cell aggregates as “pressuroid.” Notably, external acoustic stimulation produced by the FS device affected the pressuroids. Furthermore, the pressuroids exhibited upregulation in mechanosensitive genes and proteins, PIEZO1/2. CyclinD1 and PCNA, which are strongly associated with cell adhesion and proliferation, were elevated by PIEZO1/2. In addition, we found that pressuroids significantly increase angiogenic paracrine factor secretion, promote cell adhesion molecule expression, and enhance M2 immune modulation of Thp1 cells. Altogether, we have concluded that our pressuroid would suggest a more effective therapy method for future cell therapy than the conventional one. |
| first_indexed | 2024-04-10T00:36:47Z |
| format | Article |
| id | doaj.art-d28632d50ca04e928fd17be1c75a25fc |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-04-10T00:36:47Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-d28632d50ca04e928fd17be1c75a25fc2023-03-14T16:53:48ZengWileyBioengineering & Translational Medicine2380-67612023-03-0182n/an/a10.1002/btm2.10438Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesisGwang‐Bum Im0Yu‐Jin Kim1Tae Il Lee2Suk Ho Bhang3School of Chemical Engineering, Sungkyunkwan University Suwon Republic of KoreaSchool of Chemical Engineering, Sungkyunkwan University Suwon Republic of KoreaDepartment of Materials Science and Engineering Gachon University Seongnam Republic of KoreaSchool of Chemical Engineering, Sungkyunkwan University Suwon Republic of KoreaAbstract Conventional 3D cell culture methods require a comprehensive complement in labor‐intensive and time‐consuming processes along with in vivo circumstantial mimicking. Here, we describe a subaqueous free‐standing 3D cell culture (FS) device that can induce the omnidirectional environment and generate ultrafast human adipose‐derived stem cells (hADSCs) that efficiently aggregate with compaction using acoustic pressure. The cell culture conditions were optimized using the FS device and identified the underlying molecular mechanisms. Unique phenomena in cell aggregation have led to extraordinary cellular behavior that can upregulate cell compaction, mechanosensitive immune control, and therapeutic angiogenesis. Therefore, we designated the resulting cell aggregates as “pressuroid.” Notably, external acoustic stimulation produced by the FS device affected the pressuroids. Furthermore, the pressuroids exhibited upregulation in mechanosensitive genes and proteins, PIEZO1/2. CyclinD1 and PCNA, which are strongly associated with cell adhesion and proliferation, were elevated by PIEZO1/2. In addition, we found that pressuroids significantly increase angiogenic paracrine factor secretion, promote cell adhesion molecule expression, and enhance M2 immune modulation of Thp1 cells. Altogether, we have concluded that our pressuroid would suggest a more effective therapy method for future cell therapy than the conventional one.https://doi.org/10.1002/btm2.10438acoustic pressureaggregationimmune modulationPIEZO1/2therapeutic efficacy |
| spellingShingle | Gwang‐Bum Im Yu‐Jin Kim Tae Il Lee Suk Ho Bhang Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis Bioengineering & Translational Medicine acoustic pressure aggregation immune modulation PIEZO1/2 therapeutic efficacy |
| title | Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis |
| title_full | Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis |
| title_fullStr | Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis |
| title_full_unstemmed | Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis |
| title_short | Subaqueous free‐standing 3D cell culture system for ultrafast cell compaction, mechano‐inductive immune control, and improving therapeutic angiogenesis |
| title_sort | subaqueous free standing 3d cell culture system for ultrafast cell compaction mechano inductive immune control and improving therapeutic angiogenesis |
| topic | acoustic pressure aggregation immune modulation PIEZO1/2 therapeutic efficacy |
| url | https://doi.org/10.1002/btm2.10438 |
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