Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment
Due to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2019.00480/full |
| _version_ | 1811199035863728128 |
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| author | Fabiola Lilí Sarmiento-Salinas Fabiola Lilí Sarmiento-Salinas Alam Delgado-Magallón Alam Delgado-Magallón José Benito Montes-Alvarado Dalia Ramírez-Ramírez Juan Carlos Flores-Alonso Paulina Cortés-Hernández Julio Reyes-Leyva Irma Herrera-Camacho Maricruz Anaya-Ruiz Rosana Pelayo Lourdes Millán-Pérez-Peña Paola Maycotte |
| author_facet | Fabiola Lilí Sarmiento-Salinas Fabiola Lilí Sarmiento-Salinas Alam Delgado-Magallón Alam Delgado-Magallón José Benito Montes-Alvarado Dalia Ramírez-Ramírez Juan Carlos Flores-Alonso Paulina Cortés-Hernández Julio Reyes-Leyva Irma Herrera-Camacho Maricruz Anaya-Ruiz Rosana Pelayo Lourdes Millán-Pérez-Peña Paola Maycotte |
| author_sort | Fabiola Lilí Sarmiento-Salinas |
| collection | DOAJ |
| description | Due to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS). ROS are known to have a controversial role during cancer initiation and progression and although several studies have tried to manipulate intracellular ROS levels using antioxidants or pro-oxidation conditions, it is not yet clear how to target oxidation for cancer therapy. In this study, we found differences in mitochondrial morphology in breast cancer cells when compared to a non-tumorigenic cell line and differences in mitochondrial function among breast cancer subtypes when exploring gene-expression data from the TCGA tumor dataset. Interestingly, we found increased ROS levels in triple negative breast cancer (TNBC) cell lines and a dependency on ROS for survival since antioxidant treatment induced cell death in TNBC cells but not in an estrogen receptor positive (ER+) cell line. Moreover, we identified the mitochondria as the main source of ROS in TNBC cell lines. Our results indicate a potential use for ROS as a target for therapy in the TNBC subtype which currently has the worst prognosis among all breast cancers and remains as the only breast cancer subtype which lacks a targeted therapy. |
| first_indexed | 2024-04-12T01:41:07Z |
| format | Article |
| id | doaj.art-d288283853f4474fb89bcfd359ccfbcf |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-12T01:41:07Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-d288283853f4474fb89bcfd359ccfbcf2022-12-22T03:53:12ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2019-06-01910.3389/fonc.2019.00480463964Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant TreatmentFabiola Lilí Sarmiento-Salinas0Fabiola Lilí Sarmiento-Salinas1Alam Delgado-Magallón2Alam Delgado-Magallón3José Benito Montes-Alvarado4Dalia Ramírez-Ramírez5Juan Carlos Flores-Alonso6Paulina Cortés-Hernández7Julio Reyes-Leyva8Irma Herrera-Camacho9Maricruz Anaya-Ruiz10Rosana Pelayo11Lourdes Millán-Pérez-Peña12Paola Maycotte13Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoPosgrado en Ciencias Químicas, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoDepartamento de Bioquímica, Instituto Tecnológico de Acapulco, Acapulco de Juárez, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoCentro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCONACYT-Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Puebla, MexicoDue to their crucial role in cell metabolism and homeostasis, alterations in mitochondrial biology and function have been related to the progression of diverse diseases including cancer. One of the consequences associated to mitochondrial dysfunction is the production of reactive oxygen species (ROS). ROS are known to have a controversial role during cancer initiation and progression and although several studies have tried to manipulate intracellular ROS levels using antioxidants or pro-oxidation conditions, it is not yet clear how to target oxidation for cancer therapy. In this study, we found differences in mitochondrial morphology in breast cancer cells when compared to a non-tumorigenic cell line and differences in mitochondrial function among breast cancer subtypes when exploring gene-expression data from the TCGA tumor dataset. Interestingly, we found increased ROS levels in triple negative breast cancer (TNBC) cell lines and a dependency on ROS for survival since antioxidant treatment induced cell death in TNBC cells but not in an estrogen receptor positive (ER+) cell line. Moreover, we identified the mitochondria as the main source of ROS in TNBC cell lines. Our results indicate a potential use for ROS as a target for therapy in the TNBC subtype which currently has the worst prognosis among all breast cancers and remains as the only breast cancer subtype which lacks a targeted therapy.https://www.frontiersin.org/article/10.3389/fonc.2019.00480/fullbreast cancerROSmitochondriamitochondrial morphologymitochondrial ROS |
| spellingShingle | Fabiola Lilí Sarmiento-Salinas Fabiola Lilí Sarmiento-Salinas Alam Delgado-Magallón Alam Delgado-Magallón José Benito Montes-Alvarado Dalia Ramírez-Ramírez Juan Carlos Flores-Alonso Paulina Cortés-Hernández Julio Reyes-Leyva Irma Herrera-Camacho Maricruz Anaya-Ruiz Rosana Pelayo Lourdes Millán-Pérez-Peña Paola Maycotte Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment Frontiers in Oncology breast cancer ROS mitochondria mitochondrial morphology mitochondrial ROS |
| title | Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment |
| title_full | Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment |
| title_fullStr | Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment |
| title_full_unstemmed | Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment |
| title_short | Breast Cancer Subtypes Present a Differential Production of Reactive Oxygen Species (ROS) and Susceptibility to Antioxidant Treatment |
| title_sort | breast cancer subtypes present a differential production of reactive oxygen species ros and susceptibility to antioxidant treatment |
| topic | breast cancer ROS mitochondria mitochondrial morphology mitochondrial ROS |
| url | https://www.frontiersin.org/article/10.3389/fonc.2019.00480/full |
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