Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid
Our previous studies have shown that ARA regulated the gonadal steroidogenesis and the synthesis of gonadotrophin-releasing hormone (GnRH) in the brain in a gender-dependent manner in Chinese tongue sole, a marine teleost with typical sex dimorphism. As a following up-study, the current study aimed...
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmars.2019.00800/full |
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| author | Houguo Xu Houguo Xu Bo Sun Linlin Jia Yuliang Wei Yuliang Wei Zhangbin Liao Mengqing Liang Mengqing Liang |
| author_facet | Houguo Xu Houguo Xu Bo Sun Linlin Jia Yuliang Wei Yuliang Wei Zhangbin Liao Mengqing Liang Mengqing Liang |
| author_sort | Houguo Xu |
| collection | DOAJ |
| description | Our previous studies have shown that ARA regulated the gonadal steroidogenesis and the synthesis of gonadotrophin-releasing hormone (GnRH) in the brain in a gender-dependent manner in Chinese tongue sole, a marine teleost with typical sex dimorphism. As a following up-study, the current study aimed to clone and characterize a gene responding to dietary ARA differently between males and females, cholesterol 25-hydroxylase (ch25h), which has important roles in cholesterol and lipid metabolism of mammals, but is rarely investigated in fish. The full-length cDNA of Chinese tongue sole ch25h was cloned and characterized, and its transcription in the gonads, brain and liver of both males and females in response to dietary ARA levels (0.34, 2.53, and 9.63% of TFA) was investigated. The Chinese tongue sole Ch25h, which putatively is Ch25h subtype B, shared moderate identity to its known orthologs of other teleost and lower identity to human Ch25h. It has high transcription levels in the gonads, followed by skin and muscle, but low levels in the intestine, spleen, and kidney. High ARA levels significantly increased the ch25h transcription in the gonads and brain of male fish, but did not affect the transcription in the females. However, the effect of dietary ARA on ch25h transcription in the liver showed an opposite gender-difference pattern to those in the gonads and brain. To our knowledge, this is the first study in marine teleost investigating the nutritional regulation of ch25h expression. |
| first_indexed | 2024-12-16T06:52:02Z |
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| id | doaj.art-d28bf5db2f0b43a58cd9755f0870d7c4 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-16T06:52:02Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-d28bf5db2f0b43a58cd9755f0870d7c42022-12-21T22:40:22ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452020-01-01610.3389/fmars.2019.00800513165Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic AcidHouguo Xu0Houguo Xu1Bo Sun2Linlin Jia3Yuliang Wei4Yuliang Wei5Zhangbin Liao6Mengqing Liang7Mengqing Liang8Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaOur previous studies have shown that ARA regulated the gonadal steroidogenesis and the synthesis of gonadotrophin-releasing hormone (GnRH) in the brain in a gender-dependent manner in Chinese tongue sole, a marine teleost with typical sex dimorphism. As a following up-study, the current study aimed to clone and characterize a gene responding to dietary ARA differently between males and females, cholesterol 25-hydroxylase (ch25h), which has important roles in cholesterol and lipid metabolism of mammals, but is rarely investigated in fish. The full-length cDNA of Chinese tongue sole ch25h was cloned and characterized, and its transcription in the gonads, brain and liver of both males and females in response to dietary ARA levels (0.34, 2.53, and 9.63% of TFA) was investigated. The Chinese tongue sole Ch25h, which putatively is Ch25h subtype B, shared moderate identity to its known orthologs of other teleost and lower identity to human Ch25h. It has high transcription levels in the gonads, followed by skin and muscle, but low levels in the intestine, spleen, and kidney. High ARA levels significantly increased the ch25h transcription in the gonads and brain of male fish, but did not affect the transcription in the females. However, the effect of dietary ARA on ch25h transcription in the liver showed an opposite gender-difference pattern to those in the gonads and brain. To our knowledge, this is the first study in marine teleost investigating the nutritional regulation of ch25h expression.https://www.frontiersin.org/article/10.3389/fmars.2019.00800/fullCynoglossus semilaevisCh25hcloningcharacterizationgene expressionarachidonic acid |
| spellingShingle | Houguo Xu Houguo Xu Bo Sun Linlin Jia Yuliang Wei Yuliang Wei Zhangbin Liao Mengqing Liang Mengqing Liang Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid Frontiers in Marine Science Cynoglossus semilaevis Ch25h cloning characterization gene expression arachidonic acid |
| title | Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid |
| title_full | Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid |
| title_fullStr | Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid |
| title_full_unstemmed | Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid |
| title_short | Cloning and Characterization of Cholesterol 25-Hydroxylase (ch25h) From a Marine Teleost, Chinese Tongue Sole (Cynoglossus semilaevis), and Its Gene Expressions in Response to Dietary Arachidonic Acid |
| title_sort | cloning and characterization of cholesterol 25 hydroxylase ch25h from a marine teleost chinese tongue sole cynoglossus semilaevis and its gene expressions in response to dietary arachidonic acid |
| topic | Cynoglossus semilaevis Ch25h cloning characterization gene expression arachidonic acid |
| url | https://www.frontiersin.org/article/10.3389/fmars.2019.00800/full |
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