An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus
Metabolic profiling based on <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail <i>Helix aspersa</i>. Twenty metabolites were unambiguously ident...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/10/9/360 |
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| author | Nikolay G. Vassilev Svetlana D. Simova Miroslav Dangalov Lyudmila Velkova Venceslav Atanasov Aleksandar Dolashki Pavlinka Dolashka |
| author_facet | Nikolay G. Vassilev Svetlana D. Simova Miroslav Dangalov Lyudmila Velkova Venceslav Atanasov Aleksandar Dolashki Pavlinka Dolashka |
| author_sort | Nikolay G. Vassilev |
| collection | DOAJ |
| description | Metabolic profiling based on <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail <i>Helix aspersa</i>. Twenty metabolites were unambiguously identified by <sup>1</sup>H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from <i>Helix aspersa</i>. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry. |
| first_indexed | 2024-03-10T16:38:14Z |
| format | Article |
| id | doaj.art-d28c190c07da4a14a9c80f630a3c0776 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T16:38:14Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-d28c190c07da4a14a9c80f630a3c07762023-11-20T12:16:20ZengMDPI AGMetabolites2218-19892020-09-0110936010.3390/metabo10090360An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> MucusNikolay G. Vassilev0Svetlana D. Simova1Miroslav Dangalov2Lyudmila Velkova3Venceslav Atanasov4Aleksandar Dolashki5Pavlinka Dolashka6Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaInstitute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 9, Acad. G. Bonchev Str., 1113 Sofia, BulgariaMetabolic profiling based on <sup>1</sup>H nuclear magnetic resonance (NMR) spectroscopy was applied with the aim to investigate the functional role of the metabolites in lyophilized mucus from the garden snail <i>Helix aspersa</i>. Twenty metabolites were unambiguously identified by <sup>1</sup>H, 1D TOCSY, 2D J-resolved, 2D COSY, and 2D HSQC NMR spectra with water suppression. The metabolic profiles of two fractions with low molecular weight (Mw < 1 kDa and Mw < 3 kDa) are very similar. Metabolites with known antioxidant, antibacterial, and antimicrobial activity were detected by NMR metabolic analysis of mucus samples from <i>Helix aspersa</i>. Some of them were confirmed by mass spectrometric analysis. The primary structure of several peptides was identified in low molecular weight fractions (Mw < 1 kDa) by tandem mass spectrometry.https://www.mdpi.com/2218-1989/10/9/360mucusgarden snail <i>Helix aspersa</i><sup>1</sup>H NMRmetabolitesmass spectrometry |
| spellingShingle | Nikolay G. Vassilev Svetlana D. Simova Miroslav Dangalov Lyudmila Velkova Venceslav Atanasov Aleksandar Dolashki Pavlinka Dolashka An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus Metabolites mucus garden snail <i>Helix aspersa</i> <sup>1</sup>H NMR metabolites mass spectrometry |
| title | An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus |
| title_full | An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus |
| title_fullStr | An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus |
| title_full_unstemmed | An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus |
| title_short | An <sup>1</sup>H NMR- and MS-Based Study of Metabolites Profiling of Garden Snail <i>Helix aspersa</i> Mucus |
| title_sort | sup 1 sup h nmr and ms based study of metabolites profiling of garden snail i helix aspersa i mucus |
| topic | mucus garden snail <i>Helix aspersa</i> <sup>1</sup>H NMR metabolites mass spectrometry |
| url | https://www.mdpi.com/2218-1989/10/9/360 |
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