Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay

Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium ooc...

Full description

Bibliographic Details
Main Authors:	Mohammad-Reza Mahmoudi, Mojgan Bandepour, Bahram Kazemi, Asad Mirzaei
Format:	Article
Language:	English
Published:	Ilam University of Medical Sciences 2017-06-01
Series:	Journal of Basic Research in Medical Sciences
Subjects:	Cryptosporidium spp. Water Iran Real-time PCR
Online Access:	http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-58-2&slc_lang=en&sid=1

Similar Items

A Nested-PCR Assay for Detection of Cryptosporidium parvum Oocysts in Water Samples
by: M Nikaeen, et al.
Published: (2005-03-01)

Performance of diagnostic assays used to detect Cryptosporidium oocysts in faecal samples of cattle in Kuwait and genotyping of Cryptosporidium species
by: Nadra-Elwgoud M. I. Abdou, et al.
Published: (2022-09-01)

Methods used for concentrating oocysts of Cryptosporidium spp., spores Encephalitozoon spp. and Enterocytozoon spp. and their occurrence in Slovak water samples
by: Monika Sučik, et al.
Published: (2023-06-01)

Development of Sensitive Detection of Cryptosporidium and Giardia from Surface Water in Iran
by: N Taghipour Lailabadi, et al.
Published: (2011-09-01)

Recognition of Cryptosporidium oocysts in fresh and old stool samples: comparison of four techniques
by: Salman Ghaffari, et al.
Published: (2014-07-01)

Effectiveness of Cryptosporidium spp. oocysts detection and enumeration methods in water and milk samples Eficácia de métodos de detecção e enumeração de oocistos de Cryptosporidium spp. em amostras de água e leite
by: E.C.L. Machado, et al.
Published: (2006-06-01)

A Simple Alcohol-based Method of Oocyst Inactivation for Use in the Development of Detection Assays for Cryptosporidium
by: Biniam Hagos, et al.
Published: (2022-06-01)

Prevalence of Cryptosporidium spp. oocysts in dogs in Lusaka district of Zambia
by: Lamson Mugala, et al.
Published: (2018-05-01)

Synchronous Identification of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. in Stool Samples Using a Multiplex PCR Assay
by: Amir BAIRAMI, et al.
Published: (2018-03-01)

Cryptosporidium Infection in Patients with Gastroenteritis in Sari, Iran
by: Shirzad Gholami, et al.
Published: (2014-06-01)

Molecular detection of Cryptosporidium as a zoonotic pathogen, in pet birds of Isfahan, Iran
by: Mohammad Ahmadi Gharacheh, et al.
Published: (2020-06-01)

Molecular and Parasitological Study of Cryptosporidium Isolates From Cattle in Ilam, West of Iran
by: Mahmoud Mahami Oskouei, et al.
Published: (2014-09-01)

Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis of <i>Cryptosporidium</i> spp. in Stool Samples
by: Felix Weinreich, et al.
Published: (2021-09-01)

Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR
by: Ian D. Woolsey, et al.
Published: (2019-12-01)

The prevalence of Cryptosporidium spp. in lambs and goat kids in Kurdistan, Iran
by: Mohammad Khezri, et al.
Published: (2013-12-01)

Movement of Cryptosporidium parvum Oocysts through Soils without Preferential Pathways: Exploratory Test
by: Christophe J. G. Darnault, et al.
Published: (2017-06-01)

Antigen incorporation on Cryptosporidium parvum oocyst walls
by: Entrala Emilio, et al.
Published: (2001-01-01)

Utilizing Geographic Information System in Evaluation of Spatial Distribution and Epidemiology of Cryptosporidium Species in Diarrheic Humans in Mazandaran Province, North of Iran
by: Masoud Soosaraei, et al.
Published: (2021-01-01)

High prevalence of Cryptosporidium infection in Iranian patients suffering from colorectal cancer
by: Koroush Ghanadi, et al.
Published: (2022-11-01)

PRIMER REPORTE DE Cryptosporidium parvum EN TERNEROS HOLSTEIN (Bos taurus) DE MANIZALES, CALDAS, COLOMBIA4
by: R. J. Ocampo, et al.
Published: (2012-01-01)

Detection and Molecular Characterization of Cryptosporidium Species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran Using Nested-PCR-RFLP
by: K Manouchehri Naeini, et al.
Published: (2011-03-01)

Detection and Molecular Characterization of Cryptosporidium species in Recreational Waters of Chaharmahal va Bakhtiyari Province of Iran using nested-PCR-RFLP
by: K Manouchehri Naeini, et al.
Published: (2011-03-01)

Concurrent infections of cryptosporidium and giardia in dairy farms, Mississippi State, Usa
by: Queila Surumay Vílchez, et al.
Published: (2010-07-01)

A Sensitive and Specific PCR Based Method for Identification of Cryptosporidium Sp. Using New Primers from 18S Ribosomal RNA
by: M Heydarnezhadi, et al.
Published: (2011-09-01)

Molecular identification of Cryptosporidium andersoni in healthy and in cattle with diarrhea of Mashhad, Northeast of Iran
by: Alireza Sadrebazzaz
Published: (2020-04-01)

Cryptosporidium infection in Brazil: implications for veterinary medicine and public health Infecção por Cryptosporidium no Brasil: implicações em medicina veterinária e em saúde pública
by: Marcelo Vasconcelos Meireles
Published: (2010-12-01)

Cryptosporidium spp. infection in Iranian children and immunosuppressive patients: A systematic review and meta-analysis
by: Narges Kalantari, et al.
Published: (2018-01-01)

Occurrence of Cryptosporidium oocysts in commercial oysters in southern Thailand
by: Mayuna Srisuphanunt, et al.
Published: (2023-09-01)

Enrichment and proteomic identification of Cryptosporidium parvum oocyst wall
by: Luyang Wang, et al.
Published: (2022-09-01)

EVALUATION OF DIFFERENTIAL EFFICIENCY OF STAINING METHODS FOR CRYPTOSPORIDIUM OOCYSTS
by: Iakovenko D. V., et al.
Published: (2018-11-01)

Isolation of Small Number of Cryptosporidium parvum Oocyst Using Immunochromatography
by: Elahe Ebrahimzade, et al.
Published: (2014-12-01)

Cryptosporidium varanii infection in leopard geckos (Eublepharis macularius) in Argentina
by: A. Dellarupe, et al.
Published: (2016-06-01)

Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection of Cryptosporidium spp., Giardia duodenalis, and Entamoeba histolytica
by: Alejandro Dashti, et al.
Published: (2022-06-01)

Cryptosporidium parvum and Cryptosporidium andersoni infection in naturally infected cattle of northwest Iran
by: Yousef Mirzai, et al.
Published: (2014-04-01)

Contamination of Cryptosporidium spp. Oocysts in Raw Vegetables Produced in Koya City, Iraq
by: H. Sleman Ali, et al.
Published: (2018-09-01)

Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
by: M. Adamska, et al.
Published: (2012-05-01)

Contamination of environmental objects with Cryptosporidium spp. oocysts in industrial pig farm and measures to control them
by: A. S. Novikov, et al.
Published: (2021-09-01)

COMPARISON OF EFFICIENCY OF STAINING WITH ZIEHL-NEELSEN MODIFIED AND SAFRANIN MODIFIED IN DETECTION OF Cryptosporidium spp. (EUCOCCIDIORIDA, CRYPTOSPORIDIIDAE) FROM FECAL SAMPLES OF CALVES FROM 0 AND 3 MONTHS
by: Renata Dias Rodrigues, et al.
Published: (2016-01-01)

Detection and Identification of Cryptosporidium in Raw Urban Wastewaters Entering the Bu-shehr Coastal Area by Nested-PCR
by: Farshid Soleimani, et al.
Published: (2023-09-01)

Molecular evaluation of Cryptosporidium spp. among breeding calves of Lorestan province Western Iran
by: Razieh Talebi, et al.
Published: (2023-01-01)