MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution
Abstract The sequencing of single protein molecules using nanopores is faced with a huge challenge due to the lack of resolution needed to resolve single amino acids. Here we report the direct experimental identification of single amino acids in nanopores. With atomically engineered regions of sensi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-38627-x |
| _version_ | 1797822837125283840 |
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| author | Fushi Wang Chunxiao Zhao Pinlong Zhao Fanfan Chen Dan Qiao Jiandong Feng |
| author_facet | Fushi Wang Chunxiao Zhao Pinlong Zhao Fanfan Chen Dan Qiao Jiandong Feng |
| author_sort | Fushi Wang |
| collection | DOAJ |
| description | Abstract The sequencing of single protein molecules using nanopores is faced with a huge challenge due to the lack of resolution needed to resolve single amino acids. Here we report the direct experimental identification of single amino acids in nanopores. With atomically engineered regions of sensitivity comparable to the size of single amino acids, MoS2 nanopores provide a sub-1 Dalton resolution for discriminating the chemical group difference of single amino acids, including recognizing the amino acid isomers. This ultra-confined nanopore system is further used to detect the phosphorylation of individual amino acids, demonstrating its capability for reading post-translational modifications. Our study suggests that a sub-nanometer engineered pore has the potential to be applied in future chemical recognition and de novo protein sequencing at the single-molecule level. |
| first_indexed | 2024-03-13T10:13:59Z |
| format | Article |
| id | doaj.art-d28d819a2a854ce1ad2d62147f3081e4 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T10:13:59Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-d28d819a2a854ce1ad2d62147f3081e42023-05-21T11:20:09ZengNature PortfolioNature Communications2041-17232023-05-011411810.1038/s41467-023-38627-xMoS2 nanopore identifies single amino acids with sub-1 Dalton resolutionFushi Wang0Chunxiao Zhao1Pinlong Zhao2Fanfan Chen3Dan Qiao4Jiandong Feng5Laboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityLaboratory of Experimental Physical Biology, Department of Chemistry, Zhejiang UniversityAbstract The sequencing of single protein molecules using nanopores is faced with a huge challenge due to the lack of resolution needed to resolve single amino acids. Here we report the direct experimental identification of single amino acids in nanopores. With atomically engineered regions of sensitivity comparable to the size of single amino acids, MoS2 nanopores provide a sub-1 Dalton resolution for discriminating the chemical group difference of single amino acids, including recognizing the amino acid isomers. This ultra-confined nanopore system is further used to detect the phosphorylation of individual amino acids, demonstrating its capability for reading post-translational modifications. Our study suggests that a sub-nanometer engineered pore has the potential to be applied in future chemical recognition and de novo protein sequencing at the single-molecule level.https://doi.org/10.1038/s41467-023-38627-x |
| spellingShingle | Fushi Wang Chunxiao Zhao Pinlong Zhao Fanfan Chen Dan Qiao Jiandong Feng MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution Nature Communications |
| title | MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution |
| title_full | MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution |
| title_fullStr | MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution |
| title_full_unstemmed | MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution |
| title_short | MoS2 nanopore identifies single amino acids with sub-1 Dalton resolution |
| title_sort | mos2 nanopore identifies single amino acids with sub 1 dalton resolution |
| url | https://doi.org/10.1038/s41467-023-38627-x |
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