Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food
Maximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expensive instrumental methods are commonly used. Advantages of immunochromatographic analyses are their low...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2020.1750570 |
| _version_ | 1819143129891078144 |
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| author | Anastasiya V. Bartosh Alexandr Е. Urusov Alina V. Petrakova Hua Kuang Anatoly V. Zherdev Boris B. Dzantiev |
| author_facet | Anastasiya V. Bartosh Alexandr Е. Urusov Alina V. Petrakova Hua Kuang Anatoly V. Zherdev Boris B. Dzantiev |
| author_sort | Anastasiya V. Bartosh |
| collection | DOAJ |
| description | Maximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expensive instrumental methods are commonly used. Advantages of immunochromatographic analyses are their low cost and simple sample preparation; however, their sensitivity needs to be increased to contend with instrumental methods. A scheme for competitive immunochromatography with indirect labelling was implemented and developed for the detection of mycotoxin zearalenone (ZEA). Two separate reagents were used for the assay, namely free specific antibodies and antispecies antibodies conjugated with gold nanoparticles. This made it possible to simultaneously increase the sensitivity of the assay and the reliability of measurements. The instrumental detection limit of ZEA in baby food was 5 pg/mL (100 pg/g). Thus, the sensitivity attained is comparable with liquid chromatography characteristics. The duration of the analysis was 17 min. |
| first_indexed | 2024-12-22T12:21:20Z |
| format | Article |
| id | doaj.art-d28e67fef56446bf9c0b443f696eeabc |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-22T12:21:20Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-d28e67fef56446bf9c0b443f696eeabc2022-12-21T18:25:57ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432020-01-0131165366610.1080/09540105.2020.17505701750570Highly sensitive lateral flow test with indirect labelling for zearalenone in baby foodAnastasiya V. Bartosh0Alexandr Е. Urusov1Alina V. Petrakova2Hua Kuang3Anatoly V. Zherdev4Boris B. Dzantiev5A.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of SciencesSchool of Food Science and Technology, Jiangnan UniversityA.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of SciencesA.N. Bach Institute of Biochemistry, Federal Research Centre ‘Fundamentals of Biotechnology’ of the Russian Academy of SciencesMaximum permissible levels of mycotoxins in baby food may be 1% of those in ordinary food. Therefore, highly sensitive methods of mycotoxin control are in demand. To detect such low amounts, expensive instrumental methods are commonly used. Advantages of immunochromatographic analyses are their low cost and simple sample preparation; however, their sensitivity needs to be increased to contend with instrumental methods. A scheme for competitive immunochromatography with indirect labelling was implemented and developed for the detection of mycotoxin zearalenone (ZEA). Two separate reagents were used for the assay, namely free specific antibodies and antispecies antibodies conjugated with gold nanoparticles. This made it possible to simultaneously increase the sensitivity of the assay and the reliability of measurements. The instrumental detection limit of ZEA in baby food was 5 pg/mL (100 pg/g). Thus, the sensitivity attained is comparable with liquid chromatography characteristics. The duration of the analysis was 17 min.http://dx.doi.org/10.1080/09540105.2020.1750570immunochromatographyzearalenonetest stripbaby foodindirect labellingsensitivity enhancement |
| spellingShingle | Anastasiya V. Bartosh Alexandr Е. Urusov Alina V. Petrakova Hua Kuang Anatoly V. Zherdev Boris B. Dzantiev Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food Food and Agricultural Immunology immunochromatography zearalenone test strip baby food indirect labelling sensitivity enhancement |
| title | Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| title_full | Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| title_fullStr | Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| title_full_unstemmed | Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| title_short | Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| title_sort | highly sensitive lateral flow test with indirect labelling for zearalenone in baby food |
| topic | immunochromatography zearalenone test strip baby food indirect labelling sensitivity enhancement |
| url | http://dx.doi.org/10.1080/09540105.2020.1750570 |
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