Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope
Stem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobl...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Cambridge University Press
2022-01-01
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| Series: | Quantitative Plant Biology |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2632882822000121/type/journal_article |
| _version_ | 1811155764994113536 |
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| author | Shunji Shimadzu Tomoyuki Furuya Yasuko Ozawa Hiroo Fukuda Yuki Kondo |
| author_facet | Shunji Shimadzu Tomoyuki Furuya Yasuko Ozawa Hiroo Fukuda Yuki Kondo |
| author_sort | Shunji Shimadzu |
| collection | DOAJ |
| description | Stem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobleaching. Unlike fluorescence reporters, luminescence proteins do not require excitation light, and therefore offer an alternative reporter for long-term and quantitative spatio-temporal analysis. We established an imaging system for luciferase, which enabled monitoring cell fate marker dynamics during vascular development in a vascular cell induction system called VISUAL. Single cells expressing the cambium marker, proAtHB8:ELUC, had sharp luminescence peaks at different time points. Furthermore, dual-color luminescence imaging revealed spatio-temporal relationships between cells that differentiated into xylem or phloem, and cells that transitioned from procambium to cambium. This imaging system enables not only the detection of temporal gene expression, but also facilitates monitoring of spatio-temporal dynamics of cell identity transitions at the single cell level. |
| first_indexed | 2024-04-10T04:39:02Z |
| format | Article |
| id | doaj.art-d2928e419a994f79b484ca2f1b4cef00 |
| institution | Directory Open Access Journal |
| issn | 2632-8828 |
| language | English |
| last_indexed | 2024-04-10T04:39:02Z |
| publishDate | 2022-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Quantitative Plant Biology |
| spelling | doaj.art-d2928e419a994f79b484ca2f1b4cef002023-03-09T12:43:35ZengCambridge University PressQuantitative Plant Biology2632-88282022-01-01310.1017/qpb.2022.12Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscopeShunji Shimadzu0https://orcid.org/0000-0002-6166-0745Tomoyuki Furuya1https://orcid.org/0000-0002-0419-7520Yasuko Ozawa2Hiroo Fukuda3Yuki Kondo4https://orcid.org/0000-0003-1118-8662Graduate School of Science, The University of Tokyo, Tokyo, Japan Graduate School of Science, Kobe University, Kobe, JapanGraduate School of Science, Kobe University, Kobe, Japan College of Life Sciences, Ritsumeikan University, Kusatsu, JapanGraduate School of Science, The University of Tokyo, Tokyo, JapanDepartment of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto University of Advanced Science, Kameoka, JapanGraduate School of Science, Kobe University, Kobe, JapanStem cell fates are spatio-temporally regulated during plant development. Time-lapse imaging of fluorescence reporters is the most widely used method for spatio-temporal analysis of biological processes. However, excitation light for imaging fluorescence reporters causes autofluorescence and photobleaching. Unlike fluorescence reporters, luminescence proteins do not require excitation light, and therefore offer an alternative reporter for long-term and quantitative spatio-temporal analysis. We established an imaging system for luciferase, which enabled monitoring cell fate marker dynamics during vascular development in a vascular cell induction system called VISUAL. Single cells expressing the cambium marker, proAtHB8:ELUC, had sharp luminescence peaks at different time points. Furthermore, dual-color luminescence imaging revealed spatio-temporal relationships between cells that differentiated into xylem or phloem, and cells that transitioned from procambium to cambium. This imaging system enables not only the detection of temporal gene expression, but also facilitates monitoring of spatio-temporal dynamics of cell identity transitions at the single cell level.https://www.cambridge.org/core/product/identifier/S2632882822000121/type/journal_articleluminescence imagingspatio-temporal dynamicsvascular development |
| spellingShingle | Shunji Shimadzu Tomoyuki Furuya Yasuko Ozawa Hiroo Fukuda Yuki Kondo Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope Quantitative Plant Biology luminescence imaging spatio-temporal dynamics vascular development |
| title | Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| title_full | Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| title_fullStr | Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| title_full_unstemmed | Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| title_short | Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| title_sort | spatio temporal imaging of cell fate dynamics in single plant cells using luminescence microscope |
| topic | luminescence imaging spatio-temporal dynamics vascular development |
| url | https://www.cambridge.org/core/product/identifier/S2632882822000121/type/journal_article |
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