Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System
An immobilized liposome electrode (ILE)-based sensor was developed to quantify conformational changes of the proteins under various stress conditions. The ILE surface was characterized by using a tapping-mode atomic force microscopy (TM-AFM) to confirm surface immobilization of liposome. The uniform...
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MDPI AG
2018-01-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/1/136 |
| _version_ | 1811183888832135168 |
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| author | Hyunjong Yu Young Hwan Son Hak-Jin Kim Keesung Kim Pahn-Shick Chang Ho-Sup Jung |
| author_facet | Hyunjong Yu Young Hwan Son Hak-Jin Kim Keesung Kim Pahn-Shick Chang Ho-Sup Jung |
| author_sort | Hyunjong Yu |
| collection | DOAJ |
| description | An immobilized liposome electrode (ILE)-based sensor was developed to quantify conformational changes of the proteins under various stress conditions. The ILE surface was characterized by using a tapping-mode atomic force microscopy (TM-AFM) to confirm surface immobilization of liposome. The uniform layer of liposome was formed on the electrode. The current deviations generated based on the status of the proteins under different stress were then measured. Bovine carbonic anhydrase (CAB) and lysozyme were tested with three different conditions: native, reduced and partially denatured. For both proteins, a linear dynamic range formed between denatured concentrations and output electric current signals was able to quantify conformational changes of the proteins. The pattern recognition (PARC) technique was integrated with ILE-based sensor to perform data analysis and provided an effective method to improve the prediction of protein structural changes. The ILE-based stress sensor showed potential of leveraging the amperometric technique to manifest activity of proteins based on various external conditions. |
| first_indexed | 2024-04-11T13:05:23Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T13:05:23Z |
| publishDate | 2018-01-01 |
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| spelling | doaj.art-d2961436640f4961bfed791193e66a2f2022-12-22T04:22:49ZengMDPI AGSensors1424-82202018-01-0118113610.3390/s18010136s18010136Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor SystemHyunjong Yu0Young Hwan Son1Hak-Jin Kim2Keesung Kim3Pahn-Shick Chang4Ho-Sup Jung5Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Rural System Engineering, Seoul National University, Seoul 08826, KoreaDepartment of Biosystems Engineering and Biomaterials Science, Seoul National University, Seoul 08826, KoreaResearch Institute of Advanced Materials, Collage of Engineering, Seoul National University, Seoul 028826, KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaResearch Institute of Advanced Materials, Collage of Engineering, Seoul National University, Seoul 028826, KoreaAn immobilized liposome electrode (ILE)-based sensor was developed to quantify conformational changes of the proteins under various stress conditions. The ILE surface was characterized by using a tapping-mode atomic force microscopy (TM-AFM) to confirm surface immobilization of liposome. The uniform layer of liposome was formed on the electrode. The current deviations generated based on the status of the proteins under different stress were then measured. Bovine carbonic anhydrase (CAB) and lysozyme were tested with three different conditions: native, reduced and partially denatured. For both proteins, a linear dynamic range formed between denatured concentrations and output electric current signals was able to quantify conformational changes of the proteins. The pattern recognition (PARC) technique was integrated with ILE-based sensor to perform data analysis and provided an effective method to improve the prediction of protein structural changes. The ILE-based stress sensor showed potential of leveraging the amperometric technique to manifest activity of proteins based on various external conditions.http://www.mdpi.com/1424-8220/18/1/136immobilized-liposomeTM-AFMamperometric sensorconformational change proteinarray systempattern recognition |
| spellingShingle | Hyunjong Yu Young Hwan Son Hak-Jin Kim Keesung Kim Pahn-Shick Chang Ho-Sup Jung Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System Sensors immobilized-liposome TM-AFM amperometric sensor conformational change protein array system pattern recognition |
| title | Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System |
| title_full | Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System |
| title_fullStr | Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System |
| title_full_unstemmed | Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System |
| title_short | Amperometric Detection of Conformational Change of Proteins Using Immobilized-Liposome Sensor System |
| title_sort | amperometric detection of conformational change of proteins using immobilized liposome sensor system |
| topic | immobilized-liposome TM-AFM amperometric sensor conformational change protein array system pattern recognition |
| url | http://www.mdpi.com/1424-8220/18/1/136 |
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