The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits
Seasonal environmental shifts and improper eating habits are the important causes of diarrhea in children and growing animals. Whether adjusting feeding time at varying temperatures can modify cecal bacterial structure and improve diarrhea remains unknown. Three batches growing rabbits with two grou...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1344992/full |
| _version_ | 1827340767590350848 |
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| author | Shuai He Ke-Hao Zhang Qiong-Yu Jin Qiang-Jun Wang Qiang-Jun Wang Jie Huang Jun-Jiao Li Jun-Jiao Li Yao Guo Peng Liu Zhong-Ying Liu Dan Liu Shi-Xia Geng Qin Li Ming-Yong Li Man Liu Zhong-Hong Wu |
| author_facet | Shuai He Ke-Hao Zhang Qiong-Yu Jin Qiang-Jun Wang Qiang-Jun Wang Jie Huang Jun-Jiao Li Jun-Jiao Li Yao Guo Peng Liu Zhong-Ying Liu Dan Liu Shi-Xia Geng Qin Li Ming-Yong Li Man Liu Zhong-Hong Wu |
| author_sort | Shuai He |
| collection | DOAJ |
| description | Seasonal environmental shifts and improper eating habits are the important causes of diarrhea in children and growing animals. Whether adjusting feeding time at varying temperatures can modify cecal bacterial structure and improve diarrhea remains unknown. Three batches growing rabbits with two groups per batch were raised under different feeding regimens (fed at daytime vs. nighttime) in spring, summer and winter separately, and contents were collected at six time points in 1 day and used 16S rRNA sequencing to investigate the effects of feeding regimens and season on the composition and circadian rhythms of cecum bacteria. Randomized forest regression screened 12 genera that were significantly associated with seasonal ambient temperature changes. Nighttime feeding reduced the abundance of the conditionally pathogenic bacteria Desulfovibrio and Alistipes in summer and Campylobacter in winter. And also increases the circadian rhythmic Amplicon Sequence Variants in the cecum, enhancing the rhythm of bacterial metabolic activity. This rhythmic metabolic profile of cecum bacteria may be conducive to the digestion and absorption of nutrients in the host cecum. In addition, this study has identified 9 genera that were affected by the combination of seasons and feeding time. In general, we found that seasons and feeding time and their combinations affect cecum composition and circadian rhythms, and that daytime feeding during summer and winter disrupts the balance of cecum bacteria of growing rabbits, which may adversely affect cecum health and induce diarrhea risk. |
| first_indexed | 2024-03-07T21:26:14Z |
| format | Article |
| id | doaj.art-d296b27a7d1643609b375c9c5b311d30 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-07T21:26:14Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d296b27a7d1643609b375c9c5b311d302024-02-27T04:49:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13449921344992The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits Shuai He0Ke-Hao Zhang1Qiong-Yu Jin2Qiang-Jun Wang3Qiang-Jun Wang4Jie Huang5Jun-Jiao Li6Jun-Jiao Li7Yao Guo8Peng Liu9Zhong-Ying Liu10Dan Liu11Shi-Xia Geng12Qin Li13Ming-Yong Li14Man Liu15Zhong-Hong Wu16State Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaHandan Livestock Technology Extension Station, Handan, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaNational Rabbit Industry Technology System Qingdao Comprehensive Experimental Station, Qingdao, ChinaNational Rabbit Industry Technology System Qingdao Comprehensive Experimental Station, Qingdao, ChinaState Key Laboratory of Animal Nutrition and Feeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaSeasonal environmental shifts and improper eating habits are the important causes of diarrhea in children and growing animals. Whether adjusting feeding time at varying temperatures can modify cecal bacterial structure and improve diarrhea remains unknown. Three batches growing rabbits with two groups per batch were raised under different feeding regimens (fed at daytime vs. nighttime) in spring, summer and winter separately, and contents were collected at six time points in 1 day and used 16S rRNA sequencing to investigate the effects of feeding regimens and season on the composition and circadian rhythms of cecum bacteria. Randomized forest regression screened 12 genera that were significantly associated with seasonal ambient temperature changes. Nighttime feeding reduced the abundance of the conditionally pathogenic bacteria Desulfovibrio and Alistipes in summer and Campylobacter in winter. And also increases the circadian rhythmic Amplicon Sequence Variants in the cecum, enhancing the rhythm of bacterial metabolic activity. This rhythmic metabolic profile of cecum bacteria may be conducive to the digestion and absorption of nutrients in the host cecum. In addition, this study has identified 9 genera that were affected by the combination of seasons and feeding time. In general, we found that seasons and feeding time and their combinations affect cecum composition and circadian rhythms, and that daytime feeding during summer and winter disrupts the balance of cecum bacteria of growing rabbits, which may adversely affect cecum health and induce diarrhea risk.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1344992/fullgrowing rabbitfeeding regimenscecal bacteriaseasonal variationsdiarrhea |
| spellingShingle | Shuai He Ke-Hao Zhang Qiong-Yu Jin Qiang-Jun Wang Qiang-Jun Wang Jie Huang Jun-Jiao Li Jun-Jiao Li Yao Guo Peng Liu Zhong-Ying Liu Dan Liu Shi-Xia Geng Qin Li Ming-Yong Li Man Liu Zhong-Hong Wu The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits Frontiers in Microbiology growing rabbit feeding regimens cecal bacteria seasonal variations diarrhea |
| title | The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| title_full | The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| title_fullStr | The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| title_full_unstemmed | The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| title_short | The effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| title_sort | effects of ambient temperature and feeding regimens on cecum bacteria composition and circadian rhythm in growing rabbits |
| topic | growing rabbit feeding regimens cecal bacteria seasonal variations diarrhea |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1344992/full |
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