In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation
Polyphenols, like flavonoids, have been investigated when present in intact plants or in extracts as methane mitigating dietary supplements in ruminants. The aim of the present study was to examine pure compounds in a short-term in vitro experiment using the Hohenheim Gas Test method. We focused on...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2018-08-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-201808-0001_in-vitro-bioactivity-of-various-pure-flavonoids-in-ruminal-fermentation-with-special-reference-to-methane-form.php |
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| author | Susanne Sinz Carmen Kunz Annette Liesegang Ueli Braun Svenja Marquardt Carla R. Soliva Michael Kreuzer |
| author_facet | Susanne Sinz Carmen Kunz Annette Liesegang Ueli Braun Svenja Marquardt Carla R. Soliva Michael Kreuzer |
| author_sort | Susanne Sinz |
| collection | DOAJ |
| description | Polyphenols, like flavonoids, have been investigated when present in intact plants or in extracts as methane mitigating dietary supplements in ruminants. The aim of the present study was to examine pure compounds in a short-term in vitro experiment using the Hohenheim Gas Test method. We focused on the group of the flavonoids and tested which of them had the potential to mitigate methane without negatively affecting ruminal fermentation. Eight flavonoids were tested: epicatechin, luteolin-7-glucoside, quercetin, and isoquercetin in Experiment 1; catechin, gallocatechin, epigallocatechin, and epigallocatechin gallate in Experiment 2. Tannic acid, no flavonoid but a phenolic acid with known methane mitigating properties, served as positive control, and the unsupplemented basal diet as negative control. In both experiments, each of these compounds (including tannic acid) was tested at dosages of 0.5, 5.0, and 50.0 mg/g basal diet dry matter (DM) in four runs each. Gallocatechin, tannic acid, and epigallocatechin gallate (50 mg/g DM) lowered fermentation gas formation and in vitro organic matter digestibility relative to the negative control (Experiment 2). Apart from tannic acid, epicatechin, quercetin, isoquercetin, and luteolin-7-glucoside (5 and 50 mg/g DM) reduced the amount of CH4 produced in relation to total gas produced (Experiment 1). The incubation fluid ammonia concentration was decreased with luteolin-7-glucoside and tannic acid (50 mg/g DM). From the flavonoids tested especially luteolin-7-glucoside seems to have a similar potential as tannic acid to mitigate methane and ammonia formation during ruminal fermentation in vitro, both favourable in environmental respect. These results need to be confirmed in live animals. |
| first_indexed | 2024-04-10T08:25:30Z |
| format | Article |
| id | doaj.art-d29792e1c0394210a2ebdbe394fd2ec1 |
| institution | Directory Open Access Journal |
| issn | 1212-1819 1805-9309 |
| language | English |
| last_indexed | 2024-04-10T08:25:30Z |
| publishDate | 2018-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
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| series | Czech Journal of Animal Science |
| spelling | doaj.art-d29792e1c0394210a2ebdbe394fd2ec12023-02-23T03:33:35ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092018-08-0163829330410.17221/118/2017-CJAScjs-201808-0001In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formationSusanne Sinz0Carmen Kunz1Annette Liesegang2Ueli Braun3Svenja Marquardt4Carla R. Soliva5Michael Kreuzer6ETH Zürich, Institute of Agricultural Sciences, Zürich, SwitzerlandETH Zürich, Institute of Agricultural Sciences, Zürich, SwitzerlandInstitute of Animal Nutrition, Vetsuisse Faculty, University of Zürich, Zürich, SwitzerlandDepartment of Farm Animals, Vetsuisse Faculty, University of Zürich, Zürich, SwitzerlandETH Zürich, Institute of Agricultural Sciences, Zürich, SwitzerlandETH Zürich, Institute of Agricultural Sciences, Zürich, SwitzerlandETH Zürich, Institute of Agricultural Sciences, Zürich, SwitzerlandPolyphenols, like flavonoids, have been investigated when present in intact plants or in extracts as methane mitigating dietary supplements in ruminants. The aim of the present study was to examine pure compounds in a short-term in vitro experiment using the Hohenheim Gas Test method. We focused on the group of the flavonoids and tested which of them had the potential to mitigate methane without negatively affecting ruminal fermentation. Eight flavonoids were tested: epicatechin, luteolin-7-glucoside, quercetin, and isoquercetin in Experiment 1; catechin, gallocatechin, epigallocatechin, and epigallocatechin gallate in Experiment 2. Tannic acid, no flavonoid but a phenolic acid with known methane mitigating properties, served as positive control, and the unsupplemented basal diet as negative control. In both experiments, each of these compounds (including tannic acid) was tested at dosages of 0.5, 5.0, and 50.0 mg/g basal diet dry matter (DM) in four runs each. Gallocatechin, tannic acid, and epigallocatechin gallate (50 mg/g DM) lowered fermentation gas formation and in vitro organic matter digestibility relative to the negative control (Experiment 2). Apart from tannic acid, epicatechin, quercetin, isoquercetin, and luteolin-7-glucoside (5 and 50 mg/g DM) reduced the amount of CH4 produced in relation to total gas produced (Experiment 1). The incubation fluid ammonia concentration was decreased with luteolin-7-glucoside and tannic acid (50 mg/g DM). From the flavonoids tested especially luteolin-7-glucoside seems to have a similar potential as tannic acid to mitigate methane and ammonia formation during ruminal fermentation in vitro, both favourable in environmental respect. These results need to be confirmed in live animals.https://cjas.agriculturejournals.cz/artkey/cjs-201808-0001_in-vitro-bioactivity-of-various-pure-flavonoids-in-ruminal-fermentation-with-special-reference-to-methane-form.phprumenammoniahohenheim gas testepicatechinisoquercetinluteolin-7-glucosidequercetincatechintannic acid |
| spellingShingle | Susanne Sinz Carmen Kunz Annette Liesegang Ueli Braun Svenja Marquardt Carla R. Soliva Michael Kreuzer In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation Czech Journal of Animal Science rumen ammonia hohenheim gas test epicatechin isoquercetin luteolin-7-glucoside quercetin catechin tannic acid |
| title | In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation |
| title_full | In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation |
| title_fullStr | In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation |
| title_full_unstemmed | In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation |
| title_short | In vitro bioactivity of various pure flavonoids in ruminal fermentation, with special reference to methane formation |
| title_sort | in vitro bioactivity of various pure flavonoids in ruminal fermentation with special reference to methane formation |
| topic | rumen ammonia hohenheim gas test epicatechin isoquercetin luteolin-7-glucoside quercetin catechin tannic acid |
| url | https://cjas.agriculturejournals.cz/artkey/cjs-201808-0001_in-vitro-bioactivity-of-various-pure-flavonoids-in-ruminal-fermentation-with-special-reference-to-methane-form.php |
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