Peromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO prod...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2013-04-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200172 |
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| author | Elsy Nalleli Loría-Cervera Erika Ivett Sosa-Bibiano Liliana Estefanía Villanueva-Lizama Nicole Raymonde Van Wynsberghe Silvia Beatriz Canto-Lara José Luis Batún-Cutz Fernando José Andrade-Narváez |
| author_facet | Elsy Nalleli Loría-Cervera Erika Ivett Sosa-Bibiano Liliana Estefanía Villanueva-Lizama Nicole Raymonde Van Wynsberghe Silvia Beatriz Canto-Lara José Luis Batún-Cutz Fernando José Andrade-Narváez |
| author_sort | Elsy Nalleli Loría-Cervera |
| collection | DOAJ |
| description | Peromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 102 and 2.5 x 106 promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection. |
| first_indexed | 2024-03-12T18:12:43Z |
| format | Article |
| id | doaj.art-d29a153b576e4f80bf7564ebf8e5a776 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:12:43Z |
| publishDate | 2013-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-d29a153b576e4f80bf7564ebf8e5a7762023-08-02T09:15:13ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602013-04-011082172177Elsy Nalleli Loría-CerveraErika Ivett Sosa-BibianoLiliana Estefanía Villanueva-LizamaNicole Raymonde Van WynsbergheSilvia Beatriz Canto-LaraJosé Luis Batún-CutzFernando José Andrade-NarváezPeromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 102 and 2.5 x 106 promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200172nitric oxidePeromyscus yucatanicusLeishmania (L.) mexicana |
| spellingShingle | Elsy Nalleli Loría-Cervera Erika Ivett Sosa-Bibiano Liliana Estefanía Villanueva-Lizama Nicole Raymonde Van Wynsberghe Silvia Beatriz Canto-Lara José Luis Batún-Cutz Fernando José Andrade-Narváez Memorias do Instituto Oswaldo Cruz nitric oxide Peromyscus yucatanicus Leishmania (L.) mexicana |
| topic | nitric oxide Peromyscus yucatanicus Leishmania (L.) mexicana |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200172 |