Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion
Non-resorbable dental barrier membranes entail the risk of dehiscence due to their smooth and functionally inert surfaces. Non-thermal plasma (NTP) treatment has been shown to increase the hydrophilicity of a biomaterials and could thereby enhance cellular adhesion. This study aimed to elucidate the...
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MDPI AG
2023-10-01
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| Online Access: | https://www.mdpi.com/1996-1944/16/20/6633 |
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| author | Vasudev Vivekanand Nayak Nicholas Alexander Mirsky Blaire V. Slavin Lukasz Witek Paulo G. Coelho Nick Tovar |
| author_facet | Vasudev Vivekanand Nayak Nicholas Alexander Mirsky Blaire V. Slavin Lukasz Witek Paulo G. Coelho Nick Tovar |
| author_sort | Vasudev Vivekanand Nayak |
| collection | DOAJ |
| description | Non-resorbable dental barrier membranes entail the risk of dehiscence due to their smooth and functionally inert surfaces. Non-thermal plasma (NTP) treatment has been shown to increase the hydrophilicity of a biomaterials and could thereby enhance cellular adhesion. This study aimed to elucidate the role of allyl alcohol NTP treatment of poly(tetrafluoroethylene) in its cellular adhesion. The materials (non-treated PTFE membranes (NTMem) and NTP-treated PTFE membranes (PTMem)) were subjected to characterization using scanning electron microscopy (SEM), contact angle measurements, X-ray photoelectron spectroscopy (XPS), and electron spectroscopy for chemical analysis (ESCA). Cells were seeded upon the different membranes, and cellular adhesion was analyzed qualitatively and quantitatively using fluorescence labeling and a hemocytometer, respectively. PTMem exhibited higher surface energies and the incorporation of reactive functional groups. NTP altered the surface topography and chemistry of PTFE membranes, as seen through SEM, XPS and ESCA, with partial defluorination and polymer chain breakage. Fluorescence labeling indicated significantly higher cell populations on PTMem relative to its untreated counterparts (NTMem). The results of this study support the potential applicability of allyl alcohol NTP treatment for polymeric biomaterials such as PTFE—to increase cellular adhesion for use as dental barrier membranes. |
| first_indexed | 2024-03-10T21:06:20Z |
| format | Article |
| id | doaj.art-d29c2e57f2c345618e4a3406d69958a7 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T21:06:20Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-d29c2e57f2c345618e4a3406d69958a72023-11-19T17:10:04ZengMDPI AGMaterials1996-19442023-10-011620663310.3390/ma16206633Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular AdhesionVasudev Vivekanand Nayak0Nicholas Alexander Mirsky1Blaire V. Slavin2Lukasz Witek3Paulo G. Coelho4Nick Tovar5Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USADepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USABiomaterials Division, College of Dentistry, New York University, New York, NY 10010, USADepartment of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USABiomaterials Division, College of Dentistry, New York University, New York, NY 10010, USANon-resorbable dental barrier membranes entail the risk of dehiscence due to their smooth and functionally inert surfaces. Non-thermal plasma (NTP) treatment has been shown to increase the hydrophilicity of a biomaterials and could thereby enhance cellular adhesion. This study aimed to elucidate the role of allyl alcohol NTP treatment of poly(tetrafluoroethylene) in its cellular adhesion. The materials (non-treated PTFE membranes (NTMem) and NTP-treated PTFE membranes (PTMem)) were subjected to characterization using scanning electron microscopy (SEM), contact angle measurements, X-ray photoelectron spectroscopy (XPS), and electron spectroscopy for chemical analysis (ESCA). Cells were seeded upon the different membranes, and cellular adhesion was analyzed qualitatively and quantitatively using fluorescence labeling and a hemocytometer, respectively. PTMem exhibited higher surface energies and the incorporation of reactive functional groups. NTP altered the surface topography and chemistry of PTFE membranes, as seen through SEM, XPS and ESCA, with partial defluorination and polymer chain breakage. Fluorescence labeling indicated significantly higher cell populations on PTMem relative to its untreated counterparts (NTMem). The results of this study support the potential applicability of allyl alcohol NTP treatment for polymeric biomaterials such as PTFE—to increase cellular adhesion for use as dental barrier membranes.https://www.mdpi.com/1996-1944/16/20/6633guided bone regenerationnon-thermal plasmadental membranes |
| spellingShingle | Vasudev Vivekanand Nayak Nicholas Alexander Mirsky Blaire V. Slavin Lukasz Witek Paulo G. Coelho Nick Tovar Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion Materials guided bone regeneration non-thermal plasma dental membranes |
| title | Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion |
| title_full | Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion |
| title_fullStr | Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion |
| title_full_unstemmed | Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion |
| title_short | Non-Thermal Plasma Treatment of Poly(tetrafluoroethylene) Dental Membranes and Its Effects on Cellular Adhesion |
| title_sort | non thermal plasma treatment of poly tetrafluoroethylene dental membranes and its effects on cellular adhesion |
| topic | guided bone regeneration non-thermal plasma dental membranes |
| url | https://www.mdpi.com/1996-1944/16/20/6633 |
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