Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3302873?pdf=render |
| _version_ | 1811274545724653568 |
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| author | Eric A Lefevre B Veronica Carr Charlotte F Inman Helen Prentice Ian H Brown Sharon M Brookes Fanny Garcon Michelle L Hill Munir Iqbal Ruth A Elderfield Wendy S Barclay Simon Gubbins Mick Bailey Bryan Charleston COSI |
| author_facet | Eric A Lefevre B Veronica Carr Charlotte F Inman Helen Prentice Ian H Brown Sharon M Brookes Fanny Garcon Michelle L Hill Munir Iqbal Ruth A Elderfield Wendy S Barclay Simon Gubbins Mick Bailey Bryan Charleston COSI |
| author_sort | Eric A Lefevre |
| collection | DOAJ |
| description | Following the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-adjuvanted whole virion vaccine. In this study, we assessed the immune responses generated in inbred large white pigs (Babraham line) following vaccination with these vaccines and after challenge with A(H1N1)pdm/09 virus three months post-vaccination. Both vaccines elicited strong antibody responses, which included high levels of influenza-specific IgG1 and haemagglutination inhibition titres to H1 virus. Immunisation with the adjuvanted split vaccine induced significantly higher interferon gamma production, increased frequency of interferon gamma-producing cells and proliferation of CD4(-)CD8(+) (cytotoxic) and CD4(+)CD8(+) (helper) T cells, after in vitro re-stimulation. Despite significant differences in the magnitude and breadth of immune responses in the two vaccinated and mock treated groups, similar quantities of viral RNA were detected from the nasal cavity in all pigs after live virus challenge. The present study provides support for the use of the pig as a valid experimental model for influenza infections in humans, including the assessment of protective efficacy of therapeutic interventions. |
| first_indexed | 2024-04-12T23:20:55Z |
| format | Article |
| id | doaj.art-d29ccff5d42c4d609acfb2d66e2e40c2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T23:20:55Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d29ccff5d42c4d609acfb2d66e2e40c22022-12-22T03:12:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3240010.1371/journal.pone.0032400Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.Eric A LefevreB Veronica CarrCharlotte F InmanHelen PrenticeIan H BrownSharon M BrookesFanny GarconMichelle L HillMunir IqbalRuth A ElderfieldWendy S BarclaySimon GubbinsMick BaileyBryan CharlestonCOSIFollowing the emergence and global spread of a novel H1N1 influenza virus in 2009, two A(H1N1)pdm/09 influenza vaccines produced from the A/California/07/09 H1N1 strain were selected and used for the national immunisation programme in the United Kingdom: an adjuvanted split virion vaccine and a non-adjuvanted whole virion vaccine. In this study, we assessed the immune responses generated in inbred large white pigs (Babraham line) following vaccination with these vaccines and after challenge with A(H1N1)pdm/09 virus three months post-vaccination. Both vaccines elicited strong antibody responses, which included high levels of influenza-specific IgG1 and haemagglutination inhibition titres to H1 virus. Immunisation with the adjuvanted split vaccine induced significantly higher interferon gamma production, increased frequency of interferon gamma-producing cells and proliferation of CD4(-)CD8(+) (cytotoxic) and CD4(+)CD8(+) (helper) T cells, after in vitro re-stimulation. Despite significant differences in the magnitude and breadth of immune responses in the two vaccinated and mock treated groups, similar quantities of viral RNA were detected from the nasal cavity in all pigs after live virus challenge. The present study provides support for the use of the pig as a valid experimental model for influenza infections in humans, including the assessment of protective efficacy of therapeutic interventions.http://europepmc.org/articles/PMC3302873?pdf=render |
| spellingShingle | Eric A Lefevre B Veronica Carr Charlotte F Inman Helen Prentice Ian H Brown Sharon M Brookes Fanny Garcon Michelle L Hill Munir Iqbal Ruth A Elderfield Wendy S Barclay Simon Gubbins Mick Bailey Bryan Charleston COSI Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. PLoS ONE |
| title | Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. |
| title_full | Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. |
| title_fullStr | Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. |
| title_full_unstemmed | Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. |
| title_short | Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. |
| title_sort | immune responses in pigs vaccinated with adjuvanted and non adjuvanted a h1n1 pdm 09 influenza vaccines used in human immunization programmes |
| url | http://europepmc.org/articles/PMC3302873?pdf=render |
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