Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition
<p>Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between <i>longissimus dorsi </i>(LD) mus...
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| Format: | Article |
| Language: | English |
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Ivyspring International Publisher
2009-01-01
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| Series: | International Journal of Biological Sciences |
| Online Access: | http://www.biolsci.org/v05p0028.htm |
| _version_ | 1818479619881304064 |
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| author | Xiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen |
| author_facet | Xiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen |
| author_sort | Xiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen |
| collection | DOAJ |
| description | <p>Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between <i>longissimus dorsi </i>(LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (<i>SFRS18</i>) gene, also known as SRrp130. The gene exhibited relatively higher expression levels in LD muscles with higher IMF content. A full-length cDNA sequence of pig <i>SFRS18</i> gene was obtained by <i>in silico</i> comparative cloning coupled with PCR target sequencing, while the current EST (expressed sequence tag) database supported two transcript variants of the pig gene. Differential expression of the <i>SFRS18</i> gene was further confirmed using quantitative PCR. The mRNA levels of <i>SFRS18</i> gene showed significant and positive correlation with IMF content in LD muscle (r = 0.54, P < 0.01). Collectively, these results suggest that the <i>SFRS18</i> gene is involved in the regulation of IMF deposition in pig and that it may be a useful tool in selecting animals for desired amounts of fatness for high quality pork.</p> |
| first_indexed | 2024-12-10T11:13:10Z |
| format | Article |
| id | doaj.art-d29d8b817e5f4e5787732ec481f775fe |
| institution | Directory Open Access Journal |
| issn | 1449-2288 |
| language | English |
| last_indexed | 2024-12-10T11:13:10Z |
| publishDate | 2009-01-01 |
| publisher | Ivyspring International Publisher |
| record_format | Article |
| series | International Journal of Biological Sciences |
| spelling | doaj.art-d29d8b817e5f4e5787732ec481f775fe2022-12-22T01:51:20ZengIvyspring International PublisherInternational Journal of Biological Sciences1449-22882009-01-01512833Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat depositionXiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen<p>Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between <i>longissimus dorsi </i>(LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (<i>SFRS18</i>) gene, also known as SRrp130. The gene exhibited relatively higher expression levels in LD muscles with higher IMF content. A full-length cDNA sequence of pig <i>SFRS18</i> gene was obtained by <i>in silico</i> comparative cloning coupled with PCR target sequencing, while the current EST (expressed sequence tag) database supported two transcript variants of the pig gene. Differential expression of the <i>SFRS18</i> gene was further confirmed using quantitative PCR. The mRNA levels of <i>SFRS18</i> gene showed significant and positive correlation with IMF content in LD muscle (r = 0.54, P < 0.01). Collectively, these results suggest that the <i>SFRS18</i> gene is involved in the regulation of IMF deposition in pig and that it may be a useful tool in selecting animals for desired amounts of fatness for high quality pork.</p>http://www.biolsci.org/v05p0028.htm |
| spellingShingle | Xiuxing Wang, Chunyang Xue, Xiaona Wang, Honglin Liu, Yinxue Xu, Ruqian Zhao, Zhihua Jiang, Michael V. Dodson, Jie Chen Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition International Journal of Biological Sciences |
| title | Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition |
| title_full | Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition |
| title_fullStr | Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition |
| title_full_unstemmed | Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition |
| title_short | Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition |
| title_sort | differential display of expressed genes reveals a novel function of sfrs18 in regulation of intramuscular fat deposition |
| url | http://www.biolsci.org/v05p0028.htm |
| work_keys_str_mv | AT xiuxingwangchunyangxuexiaonawanghonglinliuyinxuexuruqianzhaozhihuajiangmichaelvdodsonjiechen differentialdisplayofexpressedgenesrevealsanovelfunctionofsfrs18inregulationofintramuscularfatdeposition |