Effect of some plant growth regulators and different explants types on callus induction in saffron
Saffron (Crocus sativus L.) is the world's most expensive spice. Moreover, it is important since it contains various drug metabolites. Saffron is a triploid (2n=3X=24) and sterile plant and it does not have any viable seeds. Because of the sterility, classical breeding of this plant is limited....
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| Format: | Article |
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University of Torbat Heydarieh
2017-09-01
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| Series: | Zirā̒at va Fanāvarī-i Za̒farān |
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| Online Access: | http://saffron.torbath.ac.ir/article_43257_a84767a1013b8ffe383a657522e61204.pdf |
| _version_ | 1828376942170079232 |
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| author | Khadijeh Bagheri Pejman Azadi Mitra Gholami Massoud Mir Masoumi |
| author_facet | Khadijeh Bagheri Pejman Azadi Mitra Gholami Massoud Mir Masoumi |
| author_sort | Khadijeh Bagheri |
| collection | DOAJ |
| description | Saffron (Crocus sativus L.) is the world's most expensive spice. Moreover, it is important since it contains various drug metabolites. Saffron is a triploid (2n=3X=24) and sterile plant and it does not have any viable seeds. Because of the sterility, classical breeding of this plant is limited. Developing an efficient callus induction protocol is studied for two reasons, i.e. molecular breeding and the production of secondary metabolites. In order to provide a suitable callus induction protocol, establishment of corms was considered. Five different treatments were applied to sterilization of corms. Thin cell layer explants with approximately 1 mm thickness and typical explants with approximately 1 cm thickness were prepared from sterilized corms. For callus induction, different explants were planted in MS medium containing different concentration of 2, 4-D, BAP and NAA. Then, they were incubated in dark conditions at 20 ±20C for 3 months. The results showed that the use of Benomyl fungicide, followed by surface sterilization using sodium hypochlorite (2.5 %) was the best sterilization treatment. The highest survival rates of explants (90%) were observed in this treatment and all explants were free of contamination. The highest amount of callus induction (75%) was obtained in MS medium supplemented with 2mg/l NAA and 0/5 mg/l BAP from thin cell layer of basal corm. The same result was observed with 1mg/l 2, 4-D from typical explants of basal corm. The results obtained from this study show that the thin cell layer explants are suitable explants because of the high amount of callus formation and the advantages for gene transfer studies. The results showed that the use of Benomyl fungicide, followed by surface sterilization using sodium hypochlorite (2.5 %) was the best sterilization treatment. The highest survival rates of explants (90%) was observed in this treatment and all explants were free of contamination. The highest amount of callus induction (75%) was obtained in MS medium supplemented with 2mg/l NAA and 0/5 mg/l BAP from thin cell layer of basal corm. The same result was observed with 1mg/l 2,4-D from typical explants of basal corm. Our results showed that the thin cell layer explants are suitable explant because of high amount of callus formation and advantages for gene transfer studies. |
| first_indexed | 2024-04-14T08:07:34Z |
| format | Article |
| id | doaj.art-d29f847893f74b02811527772bf9db3a |
| institution | Directory Open Access Journal |
| issn | 2383-1529 2383-2142 |
| language | fas |
| last_indexed | 2024-04-14T08:07:34Z |
| publishDate | 2017-09-01 |
| publisher | University of Torbat Heydarieh |
| record_format | Article |
| series | Zirā̒at va Fanāvarī-i Za̒farān |
| spelling | doaj.art-d29f847893f74b02811527772bf9db3a2022-12-22T02:04:42ZfasUniversity of Torbat HeydariehZirā̒at va Fanāvarī-i Za̒farān2383-15292383-21422017-09-015323123910.22048/jsat.2017.36767.112043257Effect of some plant growth regulators and different explants types on callus induction in saffronKhadijeh Bagheri0Pejman Azadi1Mitra Gholami2Massoud Mir Masoumi3Assistant professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of ZanjanAssistant professor, Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran.MSC Graduated Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan.MS.c Faculty of Biology, Tehran University.Saffron (Crocus sativus L.) is the world's most expensive spice. Moreover, it is important since it contains various drug metabolites. Saffron is a triploid (2n=3X=24) and sterile plant and it does not have any viable seeds. Because of the sterility, classical breeding of this plant is limited. Developing an efficient callus induction protocol is studied for two reasons, i.e. molecular breeding and the production of secondary metabolites. In order to provide a suitable callus induction protocol, establishment of corms was considered. Five different treatments were applied to sterilization of corms. Thin cell layer explants with approximately 1 mm thickness and typical explants with approximately 1 cm thickness were prepared from sterilized corms. For callus induction, different explants were planted in MS medium containing different concentration of 2, 4-D, BAP and NAA. Then, they were incubated in dark conditions at 20 ±20C for 3 months. The results showed that the use of Benomyl fungicide, followed by surface sterilization using sodium hypochlorite (2.5 %) was the best sterilization treatment. The highest survival rates of explants (90%) were observed in this treatment and all explants were free of contamination. The highest amount of callus induction (75%) was obtained in MS medium supplemented with 2mg/l NAA and 0/5 mg/l BAP from thin cell layer of basal corm. The same result was observed with 1mg/l 2, 4-D from typical explants of basal corm. The results obtained from this study show that the thin cell layer explants are suitable explants because of the high amount of callus formation and the advantages for gene transfer studies. The results showed that the use of Benomyl fungicide, followed by surface sterilization using sodium hypochlorite (2.5 %) was the best sterilization treatment. The highest survival rates of explants (90%) was observed in this treatment and all explants were free of contamination. The highest amount of callus induction (75%) was obtained in MS medium supplemented with 2mg/l NAA and 0/5 mg/l BAP from thin cell layer of basal corm. The same result was observed with 1mg/l 2,4-D from typical explants of basal corm. Our results showed that the thin cell layer explants are suitable explant because of high amount of callus formation and advantages for gene transfer studies.http://saffron.torbath.ac.ir/article_43257_a84767a1013b8ffe383a657522e61204.pdfThin cell layerhormonesterilization treatmentSaffronTissue culture |
| spellingShingle | Khadijeh Bagheri Pejman Azadi Mitra Gholami Massoud Mir Masoumi Effect of some plant growth regulators and different explants types on callus induction in saffron Zirā̒at va Fanāvarī-i Za̒farān Thin cell layer hormone sterilization treatment Saffron Tissue culture |
| title | Effect of some plant growth regulators and different explants types on callus induction in saffron |
| title_full | Effect of some plant growth regulators and different explants types on callus induction in saffron |
| title_fullStr | Effect of some plant growth regulators and different explants types on callus induction in saffron |
| title_full_unstemmed | Effect of some plant growth regulators and different explants types on callus induction in saffron |
| title_short | Effect of some plant growth regulators and different explants types on callus induction in saffron |
| title_sort | effect of some plant growth regulators and different explants types on callus induction in saffron |
| topic | Thin cell layer hormone sterilization treatment Saffron Tissue culture |
| url | http://saffron.torbath.ac.ir/article_43257_a84767a1013b8ffe383a657522e61204.pdf |
| work_keys_str_mv | AT khadijehbagheri effectofsomeplantgrowthregulatorsanddifferentexplantstypesoncallusinductioninsaffron AT pejmanazadi effectofsomeplantgrowthregulatorsanddifferentexplantstypesoncallusinductioninsaffron AT mitragholami effectofsomeplantgrowthregulatorsanddifferentexplantstypesoncallusinductioninsaffron AT massoudmirmasoumi effectofsomeplantgrowthregulatorsanddifferentexplantstypesoncallusinductioninsaffron |