Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs
Grape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264840/full |
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| author | Xindong Cheng Xindong Cheng Xia Du Xia Du Yanping Liang Yanping Liang Abraham Allan Degen Xiukun Wu Xiukun Wu Kaixi Ji Kaixi Ji Qiaoxian Gao Guosheng Xin Haitao Cong Guo Yang Guo Yang |
| author_facet | Xindong Cheng Xindong Cheng Xia Du Xia Du Yanping Liang Yanping Liang Abraham Allan Degen Xiukun Wu Xiukun Wu Kaixi Ji Kaixi Ji Qiaoxian Gao Guosheng Xin Haitao Cong Guo Yang Guo Yang |
| author_sort | Xindong Cheng |
| collection | DOAJ |
| description | Grape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control diet (CD) and 10 Tan lambs fed a pelleted diet containing 8% GP (dry matter basis) for 46 days. Rumen, jejunum, cecum, and colon bacterial and archaeal composition were identified by 16S rRNA gene sequencing. Dry matter intake (DMI) was greater (p < 0.05) in the GP than CD group; however, there was no difference in average daily gain (ADG, p < 0.05) and feed conversion ratio (FCR, p < 0.05) between the two groups. The GP group had a greater abundance of Prevotella 1 and Prevotella 7 in the rumen; of Sharpe, Ruminococcaceae 2, and [Ruminococcus] gauvreauii group in the jejunum; of Ruminococcaceae UCG-014 and Romboutsia in the cecum, and Prevotella UCG-001 in the colon; but lesser Rikenellaceae RC9 gut group in the rumen and cecum, and Ruminococcaceae UCG-005 and Ruminococcaceae UCG-010 in the colon than the CD group. The pathways of carbohydrate metabolism, such as L-rhamnose degradation in the rumen, starch and glycogen degradation in the jejunum, galactose degradation in the cecum, and mixed acid fermentation and mannan degradation in the colon were up-graded; whereas, the pathways of tricarboxylic acid (TCA) cycle VIII, and pyruvate fermentation to acetone in the rumen and colon were down-graded with GP. The archaeal incomplete reductive TCA cycle was enriched in the rumen, jejunum, and colon; whereas, the methanogenesis from H2 and CO2, the cofactors of methanogenesis, including coenzyme M, coenzyme B, and factor 420 biosynthesis were decreased in the colon. The study concluded that a diet including GP at 8% DM did not affect ADG or FCR in Tan lambs. However, there were some potential benefits, such as enhancing propionate production by microbiota and pathways in the GIT, promoting B-vitamin production in the rumen, facilitating starch degradation and amino acid biosynthesis in the jejunum, and reducing methanogenesis in the colon. |
| first_indexed | 2024-03-11T21:14:15Z |
| format | Article |
| id | doaj.art-d29fed2e14144e9aa857f2b3c94f907e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T21:14:15Z |
| publishDate | 2023-09-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-d29fed2e14144e9aa857f2b3c94f907e2023-09-29T05:27:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-09-011410.3389/fmicb.2023.12648401264840Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambsXindong Cheng0Xindong Cheng1Xia Du2Xia Du3Yanping Liang4Yanping Liang5Abraham Allan Degen6Xiukun Wu7Xiukun Wu8Kaixi Ji9Kaixi Ji10Qiaoxian Gao11Guosheng Xin12Haitao Cong13Guo Yang14Guo Yang15Key Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaDesert Animal Adaptations and Husbandry, Wyler Department of Dryland Agriculture, Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer Sheva, IsraelKey Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou, ChinaKey Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaNingxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, ChinaNingxia Feed Engineering Technology Research Center, Ningxia University, Yinchuan, ChinaShandong Huakun Rural Revitalization Institute Co., Ltd., Jinan, ChinaKey Laboratory of Stress Physiology and Ecology of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, ChinaYellow River Estuary Tan Sheep Institute of Industrial Technology, Dongying, ChinaGrape pomace (GP), a by-product in wine production, is nutritious and can be used as a feed ingredient for ruminants; however, its role in shaping sheep gastrointestinal tract (GIT) microbiota is unclear. We conducted a controlled trial using a randomized block design with 10 Tan lambs fed a control diet (CD) and 10 Tan lambs fed a pelleted diet containing 8% GP (dry matter basis) for 46 days. Rumen, jejunum, cecum, and colon bacterial and archaeal composition were identified by 16S rRNA gene sequencing. Dry matter intake (DMI) was greater (p < 0.05) in the GP than CD group; however, there was no difference in average daily gain (ADG, p < 0.05) and feed conversion ratio (FCR, p < 0.05) between the two groups. The GP group had a greater abundance of Prevotella 1 and Prevotella 7 in the rumen; of Sharpe, Ruminococcaceae 2, and [Ruminococcus] gauvreauii group in the jejunum; of Ruminococcaceae UCG-014 and Romboutsia in the cecum, and Prevotella UCG-001 in the colon; but lesser Rikenellaceae RC9 gut group in the rumen and cecum, and Ruminococcaceae UCG-005 and Ruminococcaceae UCG-010 in the colon than the CD group. The pathways of carbohydrate metabolism, such as L-rhamnose degradation in the rumen, starch and glycogen degradation in the jejunum, galactose degradation in the cecum, and mixed acid fermentation and mannan degradation in the colon were up-graded; whereas, the pathways of tricarboxylic acid (TCA) cycle VIII, and pyruvate fermentation to acetone in the rumen and colon were down-graded with GP. The archaeal incomplete reductive TCA cycle was enriched in the rumen, jejunum, and colon; whereas, the methanogenesis from H2 and CO2, the cofactors of methanogenesis, including coenzyme M, coenzyme B, and factor 420 biosynthesis were decreased in the colon. The study concluded that a diet including GP at 8% DM did not affect ADG or FCR in Tan lambs. However, there were some potential benefits, such as enhancing propionate production by microbiota and pathways in the GIT, promoting B-vitamin production in the rumen, facilitating starch degradation and amino acid biosynthesis in the jejunum, and reducing methanogenesis in the colon.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264840/fullwine by-productrumenhindgutmicrobiomemethanogenesis |
| spellingShingle | Xindong Cheng Xindong Cheng Xia Du Xia Du Yanping Liang Yanping Liang Abraham Allan Degen Xiukun Wu Xiukun Wu Kaixi Ji Kaixi Ji Qiaoxian Gao Guosheng Xin Haitao Cong Guo Yang Guo Yang Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs Frontiers in Microbiology wine by-product rumen hindgut microbiome methanogenesis |
| title | Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs |
| title_full | Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs |
| title_fullStr | Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs |
| title_full_unstemmed | Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs |
| title_short | Effect of grape pomace supplement on growth performance, gastrointestinal microbiota, and methane production in Tan lambs |
| title_sort | effect of grape pomace supplement on growth performance gastrointestinal microbiota and methane production in tan lambs |
| topic | wine by-product rumen hindgut microbiome methanogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1264840/full |
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