Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM Cloning
This protocol describes how to order and directly assemble uracil-containing non-clonal DNA fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is high...
| Glavni autori: | , , , , , , , |
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| Format: | Članak |
| Jezik: | English |
| Izdano: |
Bio-protocol LLC
2017-11-01
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| Serija: | Bio-Protocol |
| Online pristup: | https://bio-protocol.org/en/bpdetail?id=2615&type=0 |