A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode
Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2004-12-01
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| Series: | Iranian Journal of Chemistry & Chemical Engineering |
| Subjects: | |
| Online Access: | http://www.ijcce.ac.ir/article_8139_e47ede4f511924512112442635373c38.pdf |
| _version_ | 1811271317255618560 |
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| author | Hedayatollah Ghourchian Ahmad Moulaie Rad Hossein Elyasvandi |
| author_facet | Hedayatollah Ghourchian Ahmad Moulaie Rad Hossein Elyasvandi |
| author_sort | Hedayatollah Ghourchian |
| collection | DOAJ |
| description | Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respond to the changes in ionic strength thereby leading to uncontrolled inaccuracies. Such interferences were effectively suppressed by here the use of differential sensor pairs. It was shown that measurements in diluted fluids are possible with the use of areference sensor having no immobilized enzyme.Specifically, the sensor constructed by covalent binding provided more reproducible responses than that prepared by electropolymerization. Namely the former preserved 80% of its initial activity over a period of 25 days. In order to provide more reproducibility,a method was developed for regeneration of the sensor surface so that, the renewed sensor would responded to urea almost as new. |
| first_indexed | 2024-04-12T22:19:00Z |
| format | Article |
| id | doaj.art-d2a6bfb0ddd64323840bad431f21da6b |
| institution | Directory Open Access Journal |
| issn | 1021-9986 1021-9986 |
| language | English |
| last_indexed | 2024-04-12T22:19:00Z |
| publishDate | 2004-12-01 |
| publisher | Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
| record_format | Article |
| series | Iranian Journal of Chemistry & Chemical Engineering |
| spelling | doaj.art-d2a6bfb0ddd64323840bad431f21da6b2022-12-22T03:14:27ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering1021-99861021-99862004-12-0123255638139A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt ElectrodeHedayatollah Ghourchian0Ahmad Moulaie Rad1Hossein Elyasvandi2Laboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, I.R. IRANLaboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, I.R. IRANLaboratory of Microanalysis, Institute of Biochemistry & Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, I.R. IRANUrease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respond to the changes in ionic strength thereby leading to uncontrolled inaccuracies. Such interferences were effectively suppressed by here the use of differential sensor pairs. It was shown that measurements in diluted fluids are possible with the use of areference sensor having no immobilized enzyme.Specifically, the sensor constructed by covalent binding provided more reproducible responses than that prepared by electropolymerization. Namely the former preserved 80% of its initial activity over a period of 25 days. In order to provide more reproducibility,a method was developed for regeneration of the sensor surface so that, the renewed sensor would responded to urea almost as new.http://www.ijcce.ac.ir/article_8139_e47ede4f511924512112442635373c38.pdfurea determinationconductometric biosensorurease immobilizationelectro-polymerizationdifferential conductometric transducerurease covalent binding |
| spellingShingle | Hedayatollah Ghourchian Ahmad Moulaie Rad Hossein Elyasvandi A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode Iranian Journal of Chemistry & Chemical Engineering urea determination conductometric biosensor urease immobilization electro-polymerization differential conductometric transducer urease covalent binding |
| title | A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode |
| title_full | A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode |
| title_fullStr | A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode |
| title_full_unstemmed | A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode |
| title_short | A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode |
| title_sort | conductometric urea biosensor by direct immobilization of urease on pt electrode |
| topic | urea determination conductometric biosensor urease immobilization electro-polymerization differential conductometric transducer urease covalent binding |
| url | http://www.ijcce.ac.ir/article_8139_e47ede4f511924512112442635373c38.pdf |
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