Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil
Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of t...
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2021.636206/full |
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| author | Vanessa Valladares Célio Pasquini Silvana C. Thiengo Monica A. Fernandez Clélia C. Mello-Silva |
| author_facet | Vanessa Valladares Célio Pasquini Silvana C. Thiengo Monica A. Fernandez Clélia C. Mello-Silva |
| author_sort | Vanessa Valladares |
| collection | DOAJ |
| description | Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of the biomass of living organisms through the interaction between the electromagnetic waves and the sample. NIRS is an innovative, rapid, and non-destructive technique that can contribute to the differentiation of species based on their chemical phenotypes. Chemical profiles were obtained by NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil. The correct identification of these species is important from an epidemiological viewpoint, given that each species has distinct biological and physiological characteristics. The present study aimed to develop a chemometric model for the interspecific and intra-specific classification of the three species, focusing on laboratory and field populations. The data were obtained from 271 live animals, including 150 snails recently collected from the field, with the remainder being raised in the laboratory. Populations were sampled at three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro (B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler® software. The intra-specific classification of the field and laboratory populations obtained accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata). The interspecific differentiation had a hit rate of 75% for the field populations and 80% for the laboratory populations. The results indicate chemical and metabolic differences between populations of the same species from the field and the laboratory. The chemical phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for medical malacology, enabling the systematic discrimination of the Biomphalaria snails that are the intermediate hosts of S. mansoni in Brazil. |
| first_indexed | 2024-12-13T23:23:50Z |
| format | Article |
| id | doaj.art-d2ab13fcc95943f7b67978e2c351fd17 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-12-13T23:23:50Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Public Health |
| spelling | doaj.art-d2ab13fcc95943f7b67978e2c351fd172022-12-21T23:27:38ZengFrontiers Media S.A.Frontiers in Public Health2296-25652021-03-01910.3389/fpubh.2021.636206636206Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in BrazilVanessa Valladares0Célio Pasquini1Silvana C. Thiengo2Monica A. Fernandez3Clélia C. Mello-Silva4Environmental Health Monitoring and Prevention Laboratory, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, BrazilChemistry Institute, Universidade Estadual de Campinas/UNICAMP, Campinas, BrazilMalacology Laboratory, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, BrazilMalacology Laboratory, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, BrazilEnvironmental Health Monitoring and Prevention Laboratory, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, BrazilNear Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the vibrational energy levels of the chemical bonds of molecules within a wavelength range of 750–2,500 nm. This simple method acquires spectra that provide qualitative and quantitative data on the chemical components of the biomass of living organisms through the interaction between the electromagnetic waves and the sample. NIRS is an innovative, rapid, and non-destructive technique that can contribute to the differentiation of species based on their chemical phenotypes. Chemical profiles were obtained by NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil. The correct identification of these species is important from an epidemiological viewpoint, given that each species has distinct biological and physiological characteristics. The present study aimed to develop a chemometric model for the interspecific and intra-specific classification of the three species, focusing on laboratory and field populations. The data were obtained from 271 live animals, including 150 snails recently collected from the field, with the remainder being raised in the laboratory. Populations were sampled at three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro (B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler® software. The intra-specific classification of the field and laboratory populations obtained accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata). The interspecific differentiation had a hit rate of 75% for the field populations and 80% for the laboratory populations. The results indicate chemical and metabolic differences between populations of the same species from the field and the laboratory. The chemical phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for medical malacology, enabling the systematic discrimination of the Biomphalaria snails that are the intermediate hosts of S. mansoni in Brazil.https://www.frontiersin.org/articles/10.3389/fpubh.2021.636206/fullnear infrared spectroscopyinnovative techniquemolluskfreshwater snailschemical phenotypeschistosomiasis |
| spellingShingle | Vanessa Valladares Célio Pasquini Silvana C. Thiengo Monica A. Fernandez Clélia C. Mello-Silva Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil Frontiers in Public Health near infrared spectroscopy innovative technique mollusk freshwater snails chemical phenotype schistosomiasis |
| title | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil |
| title_full | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil |
| title_fullStr | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil |
| title_full_unstemmed | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil |
| title_short | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil |
| title_sort | field application of nir spectroscopy for the discrimination of the biomphalaria species that are intermediate hosts of schistosoma mansoni in brazil |
| topic | near infrared spectroscopy innovative technique mollusk freshwater snails chemical phenotype schistosomiasis |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2021.636206/full |
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