The female urinary microbiota in relation to the reproductive tract microbiota
Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples fr...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
GigaScience Press
2020-11-01
|
| Series: | GigaByte |
| Online Access: | https://gigabytejournal.com/articles/9 |
| _version_ | 1797871104698613760 |
|---|---|
| author | Chen Chen Lilan Hao Weixia Wei Fei Li Liju Song Xiaowei Zhang Juanjuan Dai Zhuye Jie Jiandong Li Xiaolei Song Zirong Wang Zhe Zhang Liping Zeng Hui Du Huiru Tang Tao Zhang Huanming Yang Jian Wang Susanne Brix Karsten Kristiansen Xun Xu Ruifang Wu Huijue Jia |
| author_facet | Chen Chen Lilan Hao Weixia Wei Fei Li Liju Song Xiaowei Zhang Juanjuan Dai Zhuye Jie Jiandong Li Xiaolei Song Zirong Wang Zhe Zhang Liping Zeng Hui Du Huiru Tang Tao Zhang Huanming Yang Jian Wang Susanne Brix Karsten Kristiansen Xun Xu Ruifang Wu Huijue Jia |
| author_sort | Chen Chen |
| collection | DOAJ |
| description |
Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples from 147 Chinese women of reproductive age and explored the nature of colonization by 16S rRNA gene amplicon sequencing, quantitative real-time PCR, and live bacteria culture. To demonstrate the utility of this approach, the intra-individual Spearman’s correlation was used to explore the relationship between urine and multiple sites of the female reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Streptococcus-dominated, Lactobacillus-dominated, and diverse type were the three most common urinary bacterial community types in the cohort. Detailed comparison of the urinary microbiota with multiple sites of the female reproductive tract microbiota demonstrated that the urinary microbiota were more similar to the microbiota in the cervix and uterine cavity than to those of the vagina in the same women. Our data demonstrate the potential connectivity among microbiota in the female urogenital system and provide insight and resources for exploring diseases of the urethra and genital tract.
|
| first_indexed | 2024-04-10T00:37:53Z |
| format | Article |
| id | doaj.art-d2ac8b922ef045ff8b3a27f769e5f9c2 |
| institution | Directory Open Access Journal |
| issn | 2709-4715 |
| language | English |
| last_indexed | 2024-04-10T00:37:53Z |
| publishDate | 2020-11-01 |
| publisher | GigaScience Press |
| record_format | Article |
| series | GigaByte |
| spelling | doaj.art-d2ac8b922ef045ff8b3a27f769e5f9c22023-03-14T11:18:08ZengGigaScience PressGigaByte2709-47152020-11-0110.46471/gigabyte.9The female urinary microbiota in relation to the reproductive tract microbiotaChen Chen 0https://orcid.org/0000-0001-6788-2894Lilan Hao 1https://orcid.org/0000-0003-0198-063XWeixia Wei 2Fei Li 3https://orcid.org/0000-0002-8142-1100Liju Song 4https://orcid.org/0000-0002-0175-9807Xiaowei Zhang 5https://orcid.org/0000-0002-2446-7487Juanjuan Dai 6Zhuye Jie 7https://orcid.org/0000-0003-0833-6134Jiandong Li 8Xiaolei Song 9Zirong Wang 10Zhe Zhang 11https://orcid.org/0000-0003-3983-1226Liping Zeng 12Hui Du 13Huiru Tang 14Tao Zhang 15Huanming Yang 16https://orcid.org/0000-0002-0858-3410Jian Wang 17https://orcid.org/0000-0003-4949-4753Susanne Brix 18https://orcid.org/0000-0001-8951-6705Karsten Kristiansen 19https://orcid.org/0000-0002-6024-0917Xun Xu 20https://orcid.org/0000-0002-5338-5173Ruifang Wu 21Huijue Jia 22https://orcid.org/0000-0002-3592-126XBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, China, Department of Biology, Ole MaalØes Vej 5, University of Copenhagen, Copenhagen, DenmarkBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaBGI-Shenzhen, Shenzhen 518083, ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, China, Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research, BGI-Shenzhen, Shenzhen, ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaBGI-Shenzhen, Shenzhen 518083, ChinaBGI-Shenzhen, Shenzhen 518083, ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaBGI-Shenzhen, Shenzhen 518083, China, James D. Watson Institute of Genome Sciences, Hangzhou, ChinaBGI-Shenzhen, Shenzhen 518083, China, James D. Watson Institute of Genome Sciences, Hangzhou, ChinaDepartment of Biotechnology and Biomedicine, Technical University of Denmark, Soltofts Plads, Building 221, 2800 Kgs. Lyngby, DenmarkBGI-Shenzhen, Shenzhen 518083, China, Department of Biology, Ole MaalØes Vej 5, University of Copenhagen, Copenhagen, DenmarkBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, ChinaPeking University Shenzhen Hospital, Shenzhen 518036, China, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological diseases, Shenzhen, PR ChinaBGI-Shenzhen, Shenzhen 518083, China, China National Genebank, BGI-Shenzhen, Shenzhen 518120, China, Shenzhen Key Laboratory of Human Commensal Microorganisms and Health Research, BGI-Shenzhen, Shenzhen, China, Macau University of Science and Technology, Taipa, Macau 999078, China Human urine is traditionally considered to be sterile, and whether the urine harbours distinct microbial communities has been a matter of debate. Potential links between female urine and reproductive tract microbial communities is currently not clear. Here, we collected urine samples from 147 Chinese women of reproductive age and explored the nature of colonization by 16S rRNA gene amplicon sequencing, quantitative real-time PCR, and live bacteria culture. To demonstrate the utility of this approach, the intra-individual Spearman’s correlation was used to explore the relationship between urine and multiple sites of the female reproductive tract. PERMANOVA was also performed to explore potential correlations between the lifestyle and various clinical factors and urinary bacterial communities. Our data demonstrated distinct bacterial communities in urine, indicative of a non-sterile environment. Streptococcus-dominated, Lactobacillus-dominated, and diverse type were the three most common urinary bacterial community types in the cohort. Detailed comparison of the urinary microbiota with multiple sites of the female reproductive tract microbiota demonstrated that the urinary microbiota were more similar to the microbiota in the cervix and uterine cavity than to those of the vagina in the same women. Our data demonstrate the potential connectivity among microbiota in the female urogenital system and provide insight and resources for exploring diseases of the urethra and genital tract. https://gigabytejournal.com/articles/9 |
| spellingShingle | Chen Chen Lilan Hao Weixia Wei Fei Li Liju Song Xiaowei Zhang Juanjuan Dai Zhuye Jie Jiandong Li Xiaolei Song Zirong Wang Zhe Zhang Liping Zeng Hui Du Huiru Tang Tao Zhang Huanming Yang Jian Wang Susanne Brix Karsten Kristiansen Xun Xu Ruifang Wu Huijue Jia The female urinary microbiota in relation to the reproductive tract microbiota GigaByte |
| title | The female urinary microbiota in relation to the reproductive tract microbiota |
| title_full | The female urinary microbiota in relation to the reproductive tract microbiota |
| title_fullStr | The female urinary microbiota in relation to the reproductive tract microbiota |
| title_full_unstemmed | The female urinary microbiota in relation to the reproductive tract microbiota |
| title_short | The female urinary microbiota in relation to the reproductive tract microbiota |
| title_sort | female urinary microbiota in relation to the reproductive tract microbiota |
| url | https://gigabytejournal.com/articles/9 |
| work_keys_str_mv | AT chenchen thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lilanhao thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT weixiawei thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT feili thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lijusong thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xiaoweizhang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT juanjuandai thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zhuyejie thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT jiandongli thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xiaoleisong thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zirongwang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zhezhang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lipingzeng thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huidu thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huirutang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT taozhang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huanmingyang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT jianwang thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT susannebrix thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT karstenkristiansen thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xunxu thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT ruifangwu thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huijuejia thefemaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT chenchen femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lilanhao femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT weixiawei femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT feili femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lijusong femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xiaoweizhang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT juanjuandai femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zhuyejie femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT jiandongli femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xiaoleisong femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zirongwang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT zhezhang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT lipingzeng femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huidu femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huirutang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT taozhang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huanmingyang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT jianwang femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT susannebrix femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT karstenkristiansen femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT xunxu femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT ruifangwu femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota AT huijuejia femaleurinarymicrobiotainrelationtothereproductivetractmicrobiota |