Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation
Background: Bergamot polyphenolic fraction (PF) extract exerts a beneficial against liver steatosis. However, the fundamental processes underlying this beneficial effect of bergamot PF remain elusive. In this work, we examined the effect of bergamot PF extract on 2D and 3D hepatocyte cultures. Mater...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/16/3434 |
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| author | Angela Mirarchi Rosario Mare Vincenzo Musolino Saverio Nucera Vincenzo Mollace Arturo Pujia Tiziana Montalcini Stefano Romeo Samantha Maurotti |
| author_facet | Angela Mirarchi Rosario Mare Vincenzo Musolino Saverio Nucera Vincenzo Mollace Arturo Pujia Tiziana Montalcini Stefano Romeo Samantha Maurotti |
| author_sort | Angela Mirarchi |
| collection | DOAJ |
| description | Background: Bergamot polyphenolic fraction (PF) extract exerts a beneficial against liver steatosis. However, the fundamental processes underlying this beneficial effect of bergamot PF remain elusive. In this work, we examined the effect of bergamot PF extract on 2D and 3D hepatocyte cultures. Material and Methods: We evaluated the effect of bergamot PF in 2D and 3D cultures from rat, human hepatoma cells, and human primary hepatocytes. Results: In 2D cell culture, we demonstrated that incubation with bergamot PF decreases intracellular lipid content and is associated with an increase in expression levels of ß-oxidation genes (<i>Acox1, Pparα</i>, and <i>Ucp2</i>) and lipophagy (<i>Atg7</i>). Moreover, we confirm this effect on 3D spheroids and organoids. Conclusion: Incubation with bergamot PF reduces intracellular lipid neutral fat potentially by increasing intracellular pathways related to beta-oxidation. |
| first_indexed | 2024-03-09T03:59:52Z |
| format | Article |
| id | doaj.art-d2acc1f411ce4477b96a2c942212bb97 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T03:59:52Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-d2acc1f411ce4477b96a2c942212bb972023-12-03T14:15:01ZengMDPI AGNutrients2072-66432022-08-011416343410.3390/nu14163434Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-OxidationAngela Mirarchi0Rosario Mare1Vincenzo Musolino2Saverio Nucera3Vincenzo Mollace4Arturo Pujia5Tiziana Montalcini6Stefano Romeo7Samantha Maurotti8Department of Medical and Surgical Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Clinical and Experimental Medicine, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyIRC-FSH-Pharmaceutical Biology-Department of Health Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyIRC-FSH Department of Health Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyIRC-FSH Department of Health Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Clinical and Experimental Medicine, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Sciences, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyDepartment of Clinical and Experimental Medicine, University “Magna Græcia” of Catanzaro, 88100 Catanzaro, ItalyBackground: Bergamot polyphenolic fraction (PF) extract exerts a beneficial against liver steatosis. However, the fundamental processes underlying this beneficial effect of bergamot PF remain elusive. In this work, we examined the effect of bergamot PF extract on 2D and 3D hepatocyte cultures. Material and Methods: We evaluated the effect of bergamot PF in 2D and 3D cultures from rat, human hepatoma cells, and human primary hepatocytes. Results: In 2D cell culture, we demonstrated that incubation with bergamot PF decreases intracellular lipid content and is associated with an increase in expression levels of ß-oxidation genes (<i>Acox1, Pparα</i>, and <i>Ucp2</i>) and lipophagy (<i>Atg7</i>). Moreover, we confirm this effect on 3D spheroids and organoids. Conclusion: Incubation with bergamot PF reduces intracellular lipid neutral fat potentially by increasing intracellular pathways related to beta-oxidation.https://www.mdpi.com/2072-6643/14/16/3434citrus bergamiahuman liver organoidNAFLDnutraceuticalspheroids |
| spellingShingle | Angela Mirarchi Rosario Mare Vincenzo Musolino Saverio Nucera Vincenzo Mollace Arturo Pujia Tiziana Montalcini Stefano Romeo Samantha Maurotti Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation Nutrients citrus bergamia human liver organoid NAFLD nutraceutical spheroids |
| title | Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation |
| title_full | Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation |
| title_fullStr | Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation |
| title_full_unstemmed | Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation |
| title_short | Bergamot Polyphenol Extract Reduces Hepatocyte Neutral Fat by Increasing Beta-Oxidation |
| title_sort | bergamot polyphenol extract reduces hepatocyte neutral fat by increasing beta oxidation |
| topic | citrus bergamia human liver organoid NAFLD nutraceutical spheroids |
| url | https://www.mdpi.com/2072-6643/14/16/3434 |
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